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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Official Copelandia/Panaeolus Thread [Re: 3.A.M]
    #29233849 -

I'm still learning.  I've had the denser areas like in the other jars.  That one looked funky with the small voids in it and the color change.  I haven't seen anything quite like that on my TTBVI jars.

On another note,  I have a TTBVI that I sent to sterilized coir/liquid fertilizer mix that grew wicked fast in the jar.  Fastest time to colonize a bag that I've seen.  I had intended to capture some mycelia and put it in another millet jar or agar, but forgot and just sent the whole bag to a tub.....:frown:

If this ends up fruiting...I want to save these genetics. Preferably mycelia and not from a spore print.  Maybe I can clone a fruit and hope that it is lightning fast like this.


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I may not have stayed at a Holiday Inn Express, but I have taken 20 grams of fresh subtropicalis :smile:

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Re: Official Copelandia/Panaeolus Thread [Re: Hardhead] * 6
    #29234100 -

Ready for aoi jars
This jambo is moving faster than the ttbvi now I’m pumped


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Thank your postal workers when you see them. None of this would be possible without them.

Necessity is the mother of invention

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Re: Official Copelandia/Panaeolus Thread [Re: 3.A.M] * 1
    #29234162 -

3.A.M said:
It’s fine, just pseudo sclerotia and the other jars have it also, they all look good 👍


You just made my day:doublejointed:
Going fishing right now but later today I will spawn them all.

I never done it before because didn't see the point when using pasteurized substrate. But does anyone spawn in front of their flowhood and if so why?
Simply to decrease the risk of any contams getting in during spawning I guess? Gordo mentioned it in a video about pans.

I'm sure Treefrog will come and give me her opinion on the look of my jars as well. Treefrog been my lord and savior recently when I'm being paranoid and having questions in general:bow2:

Oh and thanks for the tip about taking a small piece of the swab and placing in the agar 3AM. Your Phantoms looking good and clean:hug:


Edited by Puduwoke (05/31/25 01:26 AM)

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Re: Official Copelandia/Panaeolus Thread [Re: Puduwoke] * 9
    #29234179 -

Okay y'all, I'm in.  Just streaked the mystery Oregon Pan.


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🅃🄴🄰🄼 🄸🄷🄾🄿


New to cultivation or the shroomery?  Observe the hitchhiker's guide

The Hitchhiker's Guide to the Shroomery



Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

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Re: Official Copelandia/Panaeolus Thread [Re: Mr Piggy] * 2
    #29234182 -

Mr Piggy said:
Okay y'all, I'm in.  Just streaked the mystery Oregon Pan.


:snoopyes:

I believe I have a print of Oregon on the way, today there is germination on the Pan. Jambo and MiB RS :rockon:


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“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Official Copelandia/Panaeolus Thread [Re: Puduwoke] * 3
    #29234294 -

On spawning in front of flow hood........

I like to think of things in terms of clean vs sterile conditions.  I do all my mushroom work under clean conditions at the very minimum.  Exception to this are winecaps that I grew with some strawberries in an outdoor greenhouse.  Once you are talking garden soil outside..you are no longer clean.

All my indoor stuff, I keep everything sterile as long as possible.  I have not fiddled with pasteurization and "beneficial thermophiles"  I do agar work in front of flow hood, agar to sterilized grain in jars in front of flow hood and then transfer grain to sterilized substrate in bags in front of flow hood.  This way the spawn can colonize the substrate under sterile conditions.  After the bag is fully colonized I transfer it to a bleach sterilized tray in front of flow hood. 

Sometimes I will case (sterilized peat moss/vermiculite) in front of flow hood immediately after putting the colonized sub into tray.  Sometimes I let it reconsolidate prior to casing.  Fully sterile time ends when the sub goes into tray and conditions are now clean.

For pans, for bulk substrate I just make up 18 quarts of 4:2:1 coir/black cow/vermiculite with a cup of gypsum.  Hydrate to field capacity and put 3 quarts in 6 mycology bags, heat seal bag, place in 941 pressure cooker at 15 psi for 3 hours.  I store these in closet until ready to use. One note on cooling the pc.  I have had bags rupture if the pc was cooled too quickly.  After 3h up I turn the heat all the way down (not off) until pressure drops to about 5 psi then I turn it all the way off.  If I turn it off immediately while still at 15 psi, I think the pc cools quicker than the bags and pressure in the bags is higher that the filters can vent quickly.  They are ready to go at the drop of a hat after they cool down. 

This has worked well for me with pans.  Cubes and ochras are more forgiving and for them I just mix a jar of spawn with hydrated coir.  I still do it in front of flow hood, but the coir is not sterile.  I have some sterilized coir with liquid fertilizer going right now for ttbvi.  Got it cased and waiting on pins. 

There are a bunch of different ways to do this.  If I had access to field manure I would look for fire fang fungus and try some pasteurization but up to this point I've had good luck with sterilization.  One successful grow of pans will give you enough to have many deep experiences.

You certainly don't need to sterilize casing, but my peat moss and verm are sitting in the garage and sterilizing can't hurt.  I just do up a batch of casing, pc it in sealed quart jars.  This is convenient because the casing is ready to go at any time and I don't have to fiddle with making small batches of casing for a single tub.  Also don't have to worry about trich sneaking into an open bag of jiffy mix. 

I'm sure this is overkill, but it sucks to see a spot of green on a monotub or a tray....

One day I'm going to explore pasteurization because it is certainly easier.


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I may not have stayed at a Holiday Inn Express, but I have taken 20 grams of fresh subtropicalis :smile:

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Re: Official Copelandia/Panaeolus Thread [Re: Hardhead] * 11
    #29235518 -



5 trays of TTBVI spawned to trays.

To say I'm excited would be a understatement :smitten:

Edited by Puduwoke (06/01/25 04:19 AM)

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Re: Official Copelandia/Panaeolus Thread [Re: Puduwoke]
    #29235548 -

Do you really need to pc AIO jars for 2 hours when you're using small cans?


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Re: Official Copelandia/Panaeolus Thread [Re: SkanskKorv] * 7
    #29235549 -

Why risk cooking less? 🤔 Eventually you need to stray from the pack though and see what actually works for you. Maybe 90 minutes is sufficient. Maybe 60 minutes is sufficient. Maybe 10 minutes is good.. ok probably not 10 minutes.

Experiment
Take notes, write down EVERYTHING. note the cook times and how they finish up all the way until you're harvesting.

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Re: Official Copelandia/Panaeolus Thread [Re: PhenoDreamers] * 2
    #29235556 -

:whathesaid:

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Re: Official Copelandia/Panaeolus Thread [Re: PhenoDreamers]
    #29235565 -

PhenoDreamers said:
Why risk cooking less? 🤔 Eventually you need to stray from the pack though and see what actually works for you. Maybe 90 minutes is sufficient. Maybe 60 minutes is sufficient. Maybe 10 minutes is good.. ok probably not 10 minutes.

Experiment
Take notes, write down EVERYTHING. note the cook times and how they finish up all the way until you're harvesting.


Yeah I'm already doing logs on everything I do to keep track but right now I don't have much time and skipping just 30-60 min could help me pull this off today and I kinda have to since I won't have any chance to do this until next weekend and my vacation is closing in fast so yeah... But I guess I'll.


I just noticed that I said 2 hours but I meant 3 hours. I meant cutting the time by an hour and cook it for 2 hours.


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Re: Official Copelandia/Panaeolus Thread [Re: SkanskKorv]
    #29235574 -

TTBVI ….

I’ve managed to get several tubs to fruit, but here’s my issue-

I know the fruits are gonna be smaller, but I’m basically growing 1” toothpicks at best.  If I am able to successfully harvest a cluster, they just dry down into sewing thread size and crumble to dust.  I’m not expecting chunky, but I know they can do better.

I’m running about 25% manure/coir.  The tubs aren’t contaminating.

Any tips?  Is it likely just genetics?  Do I need more nutrition?  Moisture?  Is there any environmental factor that keeps them little?  Thanks for any ideas.

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Re: Official Copelandia/Panaeolus Thread [Re: SkanskKorv] * 11
    #29235578 -

SkanskKorv said:
Do you really need to pc AIO jars for 2 hours when you're using small cans?


No, the prep was for 1/2 gallon jars but 2 hours is perfect for qt jars.

Getting some fatties with the tent dialed in better.  Going to try cloning this cluster.




--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: Official Copelandia/Panaeolus Thread [Re: tree frog]
    #29235583 -

tree frog said:
SkanskKorv said:
Do you really need to pc AIO jars for 2 hours when you're using small cans?


No, the prep was for 1/2 gallon jars but 2 hours is perfect for qt jars.

Getting some fatties with the tent dialed in better.  Going to try cloning this cluster.






Actually I'm doing way smaller than that even... I'm still using my old 1/4 quart pf Tek jars ( I know they're too small but they're what I had). That answers my question though so I'll be going for 2 hours, thank you! :rockon:


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Re: Official Copelandia/Panaeolus Thread [Re: SkanskKorv] * 1
    #29235609 -

Yeah, you would probably be safe with an hour with small jars. I personally would still do at least 90 minutes but 2 hours isn't going to hurt anything I imagine.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: Official Copelandia/Panaeolus Thread [Re: Triplefast] * 1
    #29235663 -

Triplefast said:
TTBVI ….

I’ve managed to get several tubs to fruit, but here’s my issue-

I know the fruits are gonna be smaller, but I’m basically growing 1” toothpicks at best.  If I am able to successfully harvest a cluster, they just dry down into sewing thread size and crumble to dust.  I’m not expecting chunky, but I know they can do better.

I’m running about 25% manure/coir.  The tubs aren’t contaminating.

Any tips?  Is it likely just genetics?  Do I need more nutrition?  Moisture?  Is there any environmental factor that keeps them little?  Thanks for any ideas.


Could be a few things. Like excess nutrients, excess moisture, inadequate FAE to name a few. Could be genes too but I’d probs scrutinize that last tbh


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CV+

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Re: Official Copelandia/Panaeolus Thread [Re: tree frog]
    #29235671 -

Even if an hour would have worked I'd do 2 because I'm paranoid I'll have any contamination left in those jars. And now it's just 6 minutes until the 2 hours have passed but next time I might try 90 min unless I've got bigger jars next time. The problem is fitting them in my pc since I don't have a very large one... But this works for now I guess except that it takes 2-3x more time to pc substrate.

Thanks once again for calming my mind


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Re: Official Copelandia/Panaeolus Thread [Re: SkanskKorv] * 13
    #29236277 -

Tea tree pans, forgive the splotching on some caps :lol:




They look kinda cute though, pretty happy with the first run all things considered. Can’t smell a damn thing right now on account of the wildfires but I’m sure they smell delectable.

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Re: Official Copelandia/Panaeolus Thread [Re: kirkeng]
    #29236288 -

kirkeng said:
Tea tree pans, forgive the splotching on some caps :lol:




They look kinda cute though, pretty happy with the first run all things considered. Can’t smell a damn thing right now on account of the wildfires but I’m sure they smell delectable.



I'm curious, what leads to them all growing to different heights like this? Just it being MS or possibly airflow or water?

And do you know if using a bit of a thicker casing layer, like 1/4-1/2 inch helps with more even growth?  I just notice parts of my trays, the mushrooms will take off growing faster than other areas and end up taller but the caps will open sometimes at the same time and other times, areas of a tray are done fruiting will another side will still have a day or two or more left.

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Re: Official Copelandia/Panaeolus Thread [Re: MojaveMyc] * 8
    #29236303 -

MojaveMyc said:
Could be a few things. Like excess nutrients, excess moisture, inadequate FAE to name a few. Could be genes too but I’d probs scrutinize that last tbh


Really, too much nutrient can lead to tiny fruit? Like too much grain or too much manure?

TTBVI is definitely a small one but in my experience with it, it can definitely grow more than an inch tall.  Too much airflow I notice keeps them shorter.  Not enough water keeps them super thin but even then, it can still be super thin.  Too much grain seems to lead to a ton of pins but smaller mushrooms and the need to water more.  Less airflow and thus higer co2 should make them reach a bit.  Mine grow usually anywhere from 2-3 inches.

I think there are genetics of it that grow taller/bigger but my grows are pretty small of it.  I've been using around a 1/2qt of grain per 9x13'ish tray (I use sterilite clipboxes).  Ratio is probably 1:3-1:4 grain to sub.  I find a full quart jar too much of it. Some plates of it are more agressive than others I notice as well.




Edited by Timestop413 (06/01/25 06:08 PM)

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Shop: Myyco.com Pan Cyan Liquid Culture For Sale   Mushroom-Hut Substrate Bags   Sporeworks.EU Spores for European Microscopy   North Spore Bulk Substrate   Original Sensible Seeds Bulk Cannabis Seeds   MagicBag.co All-In-One Bags That Don't Suck

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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