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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Spore cleaning without a centrifuge or agar [Re: PhenoDreamers]
    #29158735 -

I dont know what you consider "wide" but I have a stack of 3 cakes with 3 different cube strains a garbage bag on my deck waiting for the weather to break so I can chuck it in the garden, and it's continued to spawn pins despite freezing solid when it was 6-7 below at night. daytime temps are 18ish and while I wouldnt choose to grow at this temp, the cakes are surviving enough to seemingly make it to spring.

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Re: Spore cleaning without a centrifuge or agar [Re: dinosaurs] * 2
    #29165465 -

Lift off. Confirm pasteurization of spores survival to fruiting. While the same syringe contaminated with Penicillium.




--------------------
The better guide to grow mushrooms.
https://www.shroomery.org/forums/showflat.php/Number/8553541

Beware the power of one.  The power of two. The power of many.

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Re: Spore cleaning without a centrifuge or agar [Re: vicepope]
    #29167709 -

:yess:


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Low Prep the World! (agar plates)
Low Prep the World! (grain prep)
Trashcan FC
Acidulated extraction gummies and drops
Drying agar and grains using parchment paper
:leaf:Cleaning your bubble hash bags


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Re: Spore cleaning without a centrifuge or agar [Re: YoshiTrainer] * 4
    #29167857 -

Every time I see this thread it makes me want to learn how to clean spores with a centrifuge...

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Re: Spore cleaning without a centrifuge or agar [Re: psilygoose] * 1
    #29167939 -

:whathesaid:

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Re: Spore cleaning without a centrifuge or agar [Re: psilygoose] * 1
    #29168021 -

psilygoose said:
Every time I see this thread it makes me want to learn how to clean spores with a centrifuge...


You Spin Me Round (Like a Record)


--------------------

Check out my My Trade List  :opensign:

Check out my wanted Strain List  :opensign:



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Re: Spore cleaning without a centrifuge or agar [Re: vicepope]
    #29168267 -

vicepope said:
Lift off. Confirm pasteurization of spores survival to fruiting. While the same syringe contaminated with Penicillium.





:grannycheer:


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:ancientaliens:

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Re: Spore cleaning without a centrifuge or agar [Re: astra] * 5
    #29172427 -

I made two open air 1/4 pint jars. Each had 30 mL of water, one with a few pale semperviva prints and the other with caps that had been printed. I included another straight water jar to check temperatures. I used an instant read digital thermometer.
I used a dehydrator set to 160f. The jars took an hour to get to 160 and I ran them for a total of 3 hours and let them cool overnight.

Inoculated BRF cakes March 7, pictures are from March 28.
Jar from prints.


Jar from caps. I’m going to take the white growth to pucks. Looks like semp to me.

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Re: Spore cleaning without a centrifuge or agar [Re: The Tao] * 1
    #29173315 -


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Re: Spore cleaning without a centrifuge or agar [Re: The Tao]
    #29173345 -

Inoculated first, then exposed to heat? Am I reading that correctly?

The BRF mix alone requires longer exposure  to prevent mold, the addition of spores will exacerbate that problem. You're not going to produce anything notable by cycling nutrient dense media in a dry heat environment at those temps.

Unless I'm completely misunderstanding the post.

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Re: Spore cleaning without a centrifuge or agar [Re: Stipe-n Cap] * 1
    #29173363 -

The cap and print jars were processed in the dehydrator. The BRF cakes were processed in an instant pot. The prepped BRF cakes were inoculated by syringe the day after.

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Re: Spore cleaning without a centrifuge or agar [Re: The Tao]
    #29173425 -

Rgr, I misunderstood. Thanks for clarifying.

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Re: Spore cleaning without a centrifuge or agar [Re: The Tao] * 1
    #29173481 -

Starting round two. Same generation line fresh spore syringe making now and pasteurization the syringe with the fresh shrooms 2 qts jars gave 780 grams wet.


The Tao said:
I made two open air 1/4 pint jars. Each had 30 mL of water, one with a few pale semperviva prints and the other with caps that had been printed. I included another straight water jar to check temperatures. I used an instant read digital thermometer.
I used a dehydrator set to 160f. The jars took an hour to get to 160 and I ran them for a total of 3 hours and let them cool overnight.

Inoculated BRF cakes March 7, pictures are from March 28.
Jar from prints.


Jar from caps. I’m going to take the white growth to pucks. Looks like semp to me.



Amazing I should make cakes. To see speed gap with the old clean lc vs the pasteurization spores.


--------------------
The better guide to grow mushrooms.
https://www.shroomery.org/forums/showflat.php/Number/8553541

Beware the power of one.  The power of two. The power of many.

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Re: Spore cleaning without a centrifuge or agar [Re: vicepope]
    #29175779 -

Does a clump indicate any sign of life from spores? My instant pot test failed, but I noticed that they had clumped up in the container, so I put it on agar.



--------------------
I call them Huskies cause they Mush!


Huskies' Grimoire
The Official Wild Cubensis Thread
Easy Instant Pot Agar
Let's Talk Cowtek

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Re: Spore cleaning without a centrifuge or agar [Re: Huskies]
    #29176078 -

Update: I have been squirting the spore syringes in grain jars. 145 for one hour is still not sufficient. while it greatly slowed down growth of mold spores it did not kill them all and I observed no other growth. I'm still waiting on higher mold spore count syringes at 145 to see if they will show any sign of mycelial growth but I do not have high hopes. put the 170f to grain a few days ago, still no growth

edit: I do have all of them on agar as well, but I remembered someone brought up maybe spores would get killed as well so I am pouring the remaining liquid into grain jars. The plates that had the 145f spores showed bacterial growth, I do not know where it is coming from. any advice is appreciated greatly


--------------------
Yummy!


LAGM 2025
https://www.shroomery.org/forums/showflat.php/Number/29174693#29174693

Edited by syllable (04/02/25 02:31 AM)

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Re: Spore cleaning without a centrifuge or agar [Re: The Tao]
    #29176100 -

The Tao said:
I made two open air 1/4 pint jars. Each had 30 mL of water, one with a few pale semperviva prints and the other with caps that had been printed. I included another straight water jar to check temperatures. I used an instant read digital thermometer.
I used a dehydrator set to 160f. The jars took an hour to get to 160 and I ran them for a total of 3 hours and let them cool overnight.

Inoculated BRF cakes March 7, pictures are from March 28.
Jar from prints.


Jar from caps. I’m going to take the white growth to pucks. Looks like semp to me.



:manofapproval::heart::heart:


--------------------
:ancientaliens:

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Re: Spore cleaning without a centrifuge or agar [Re: vicepope]
    #29179917 -

this is cool but what exactly was your process. I looked through the thread and all you said was you were doing the dehydrator. unless I missed it?


--------------------
Yummy!


LAGM 2025
https://www.shroomery.org/forums/showflat.php/Number/29174693#29174693

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Re: Spore cleaning without a centrifuge or agar [Re: syllable] * 1
    #29180086 -

Interesting developments after 5 days. I put the spore clump on agar to see what would happen since the syringes I made were a fail after 45 minutes in an instant pot. Maybe a smaller time might be possible to kill mold and bacteria and still allow enough spores to survive.

You can see its original imprint, I moved it a bit after. I also did this in open air just cause I wasn't expecting it to do anything. But, it looks like I have clean mycelial growth I can transfer from, so this will be interesting.



I mixed a swab of Yeti and a spore print of Husky (a standard cube), hoping for a cross. I used the yeti swab to gather the print and put it in filtered water before PCing it. From those who are experienced with crossing, does it normally show in the F1, or do you normally need to wait til F2 to see if it crossed correctly.


--------------------
I call them Huskies cause they Mush!


Huskies' Grimoire
The Official Wild Cubensis Thread
Easy Instant Pot Agar
Let's Talk Cowtek

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Re: Spore cleaning without a centrifuge or agar [Re: Huskies] * 7
    #29182809 -

I’ve got some data. Still waiting for spores to germinate at 7 days from inoculation. It was an old spore syringe, so my fingers are crossed. I do have some growth, but not the good kind.

I took a spore syringe that was about two years old and divided it up among 10 cryovials. One was a control. The others were each heat treated. One vial at each 15 minute increments from 15 minutes to 45 minutes, at three temps 140F, 150F & 160F. I used my dehydrator as that is how I do most of my small scale pasteurization. To alleviate Stipe’s fears of spore load, each vial was placed into a 2x3 ziploc prior to being placed into the dehydrator. The sealed bags were rinsed in bleach water after removal from the dehydrator and allowed to dry before taking a sample to put to agar.

Control plate. About as bad as a MS syringe can get, but looks like all bacteria no mold.


15 min @ 140F. Eww.


30 min @ 140F. Better, but not what I had hoped for given the FDA says this is the time and temp for pasteurization.


45 min @ 140F. Still a few colonies.


15 min @ 150F. Not good.


The remaining plates at 150F, and all three at 160F have no growth at seven days. Hopefully the bacteria has been killed off and the mushroom spores will germinate. At this point I still see no mycelium on the control, so I can’t rule out just old spore not germinating instead of being killed by heat. I’ll keep these plates around a while, and I plan to make another syringe with fresh spores next time I have cubes to harvest.


--------------------
Method for Small Scale Casing Pasteurization

How much really fits in a 00 capsule?

Micro and Macro Mushroom Photography

Can you freeze mycelium?


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Re: Spore cleaning without a centrifuge or agar [Re: RoscoeReturns]
    #29182825 -

:popcorn:

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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