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Re: Psychedelicmind's warm-up grow - trial and error! [Re: psychedelicmind]
    #29102438 -

... And the last update for today:
Psilocybe cubensis var. Wollongong.
Third transfer was done on January, 10th.
The pictures were shot on January, 21st.
Not sure if these are perfectly clean.
The myc seems to keep very thin and looks a little bit powdery in some areas i think.
For comparison i have also added some pictures of the original plates. (T2)


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Re: Psychedelicmind's warm-up grow - trial and error! [Re: psychedelicmind]
    #29109921 -

The following species have not shown any signs of healthy growth until now:

Panaeolous cyanescens Jamaica
Panaeolous tropicalis
Psilocybe tampanensis

Got some LC syringes and put them onto different agar plates. MEA, MEP, PDA, Kimmig agar.
Nothing worked.
Inoculated some grain jars with the same syringes and the mycelium looked healthy within the first two weeks.
But then it got worse and looked like comtaminated.
Will inoculate some more jars next weekend and try to do some G2A transfers.
Here are some pics of some of the plates.

Panaeolous cyanescens Jamaica



Panaeolous tropicalis



Psilocybe tampanensis


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Re: Psychedelicmind's warm-up grow - trial and error! [Re: psychedelicmind]
    #29127998 -

Couldn't wait for my agar experiments to finish and inoculated some spores of Ps. cubensis 'Wollongong' into some rye grain jars.
The fruits were very tiny, i assume thats because of a lack of water.
Didn't pasteurize enough verm/coir so the casing layer got very thin and the casings dried out very quickly.
After the first flush i dunked the casing for 24 hours and gave them a good mist.
Flush two has produced way bigger fruits.
Here are some pics of flush one.


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Re: Psychedelicmind's warm-up grow - trial and error! [Re: psychedelicmind]
    #29128292 -

Looking good Psychedelic

:happyclaps:


--------------------
Hitchhiker’s Guide to the Shroomery “Clean spawn is easily the most critical component of the entire cultivation process.”
Drink the Water of Life and become Paul Muad’Dib.
Remember to surrender.

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Re: Psychedelicmind's warm-up grow - trial and error! [Re: AzureCultivator]
    #29138236 -

AzureCultivator said:
Looking good Psychedelic

:happyclaps:



Thank you, AzureCultivator!

Last weekend i made another attempt to save Psilocybe tampanensis, Panaeolus cyanescens Jamaica and Panaeolous tropicalis.
A few grain jars had been inoculated some weeks ago.
These jars looked better than the dishes i made.
I put a few colonized rye berries on agar plates.
Hope to be able to isolate some healthy myc this way.


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Re: Psychedelicmind's warm-up grow - trial and error! [Re: psychedelicmind]
    #29138276 -

Made some LC of Psilocybe cubensis 'Wollongong' and Psilocybe cyanescens.
After the jars had colonized i gave them a stir with a magnetic stirrer and let them colonize for some more time.
Then i made a few LC syringes.



A few drops of both species have been dropped on an agar dish to check if they're clean.
The jars are waiting in my fridge for further use.
My plan is to inoculate some grain jars with cyanescens myc.
These will be used to bulk to alder woodchips for an outdoor grow.


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Re: Psychedelicmind's warm-up grow - trial and error! [Re: psychedelicmind] * 1
    #29151930 -

Still having trouble getting my agar work clean.
So i decided to do some more MSS grain jars to bridge the time.
From left to right: Cubensis 'Puerto Rico' - Cubensis 'Puerto Rico' - Cubensis 'Fidgi' - Cubensis 'Fidgi'
The jars looked pure white until full colonization.
After full colonization they developed some small areas with a yellowish teint.
I assume it was metabolites because they wanted to get out of the jars.
Maybe there was also a little bit of bacteria inside because it was a MSS inoculation.
I also noticed that there was a lot of condensation inside the jars.
Maybe because of temperature fluctuations in my growing room?

Here are some pics of the shoeboxes - marsh, 4th:



And some pictures made yesterday - marsh, 8th:



And finally some pics of the jars which were used:


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Re: Psychedelicmind's warm-up grow - trial and error! [Re: psychedelicmind] * 1
    #29151955 -

The jars do not look clean of bacteria. I’m new myself and just went over this post again - Clean spawn checklist for the new grower.

For example the first jar seems to have similar rhizomorphs.

Quote:
The sort of spikey/sickly growth patterns pictured immediately below are not healthy rhizomorphs but likely an attempt by the mycelium to escape a contaminated environment:







Also they seem to go under this chapter too:

Quote:
1.Colour and consistency:  Is your mycelium an unnaturally absurd colour of white? does it have bright white splotches or very pronounced, spikey spiderweb like rhizomorphic tendrils? Does the mycelium  have a thick/smooth ice cream like consistency;

Examples of bacterial, thick/creamy white mycelium:

…






Not trying to be this guy



It’s just that i feel I learn faster myself too if I express what I’ve read. And I also wanted to share a thread that might make self identifying easier.
Also you might still get something out of these, but you can at least maybe correlate the performance with the spawn now and in the future.

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Re: Psychedelicmind's warm-up grow - trial and error! [Re: eilauver] * 1
    #29152354 -

Thanks for your feedback and the link, eilauver.
Yeah, the pics of your link look very similar to mine.  :-(

I will never again hit spores into grain.
Next experiments will be done with agar first.  :-)

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Re: Psychedelicmind's warm-up grow - trial and error! [Re: psychedelicmind]
    #29152381 -

psychedelicmind said:
Thanks for your feedback and the link, eilauver.
Yeah, the pics of your link look very similar to mine.  :-(

I will never again hit spores into grain.
Next experiments will be done with agar first.  :-)



Excellent lesson learned. Well done psychedelicmind


--------------------
Hitchhiker’s Guide to the Shroomery “Clean spawn is easily the most critical component of the entire cultivation process.”
Drink the Water of Life and become Paul Muad’Dib.
Remember to surrender.

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Re: Psychedelicmind's warm-up grow - trial and error! [Re: AzureCultivator] * 2
    #29158990 -

Update of my Cubensis 'Fidgi' and 'Puerto Rico' shoeboxes.
Unfortunately they're contaminated with bacteria, but i'm gonna let them flush to save the genetics.
Some have a nice pinset i think, some others look really sickly.
The bigger boxes got really dry and the substrate has shrunk a bit, so i gave them a bottom watering.

Fidgi:



Puerto Rico:


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Re: Psychedelicmind's warm-up grow - trial and error! [Re: psychedelicmind] * 2
    #29179547 -

After harvesting the first flush of my Puerto Rico and Fidgi shoeboxes i gave them soak but i forgot to remove the excess water for a few days. As a result they got contaminated with a white mold.
The boxes have been tossed and the whole room has been sanitized as well as possible.
In future i will use another room for working with agar.





The following species are on agar right now:

Psilocybe cubensis 'Wollongong'
Psilocybe cubensis 'Puerto Rico'
Psilocybe cubensis 'Fidgi'
Psilocybe cubensis 'Australia - blue meanie'
Psilocybe cubensis 'B+'

Psilocybe nataliensis

Psilocybe cyanescens

Psilocybe tampanensis

Panaeolus cyanescens 'Hawai'
Panaeolus cyanescens 'Jamaica'
Panaeolus tropicalis


The following species are on grain:

Psilocybe cyanescens (inoculated with LC, not spores! :grin:) - look clean to my noob eyes, will update pics before bulking to woodchips.


Shoeboxes are ready from the following species:

Psilocybe cubensis 'Wollongong' (The grain was made from LC, but i am not sure if everything is clean. Will post pictures tomorrow. In retrospect i think i should have done a few more transfers...


The following LC syringes have just arrived:

Psilocybe zapotecorum
Psilocybe mexicana 'Jalisco'



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Re: Psychedelicmind's warm-up grow - trial and error! [Re: psychedelicmind]
    #29179814 -

Nice looking stuff, wish you lots of luck! (:

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Re: Psychedelicmind's warm-up grow - trial and error! [Re: CatalieMeow]
    #29179983 -

CatalieMeow said:
Nice looking stuff, wish you lots of luck! (:



Thank you, CatalieMeow!  :smile:

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Re: Psychedelicmind's warm-up grow - trial and error! [Re: psychedelicmind]
    #29188252 -

My psilocybe cyanescens grain jars have been bulked to woodchips in flower pots on my balcony.
Pictures have been posted in 'The Official Woodlovers Thread'

https://www.shroomery.org/forums/showflat.php/Number/29187387#29187387

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Re: Psychedelicmind's warm-up grow - trial and error! [Re: psychedelicmind] * 1
    #29188254 -

Two shoeboxes of Psilocybe cubensis 'Wollongong' have been put into fruiting conditions.
They originate from a LC that i made last year.
It was my first attempt making LC from agar so i think it was not completely clean.
There are some yellowish/brownish areas visible after incubation.
More agar plates are of the same variety are in progress.
Will transfer them until they look completely clean and strong.


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Re: Psychedelicmind's warm-up grow - trial and error! [Re: psychedelicmind] * 1
    #29191134 -

These are a few pics of my T1-plates made on Marsh, 17th:

Psilocybe cubensis ‚Australia‘ – isolate ‚blue meanie‘
Psilocybe cubensis ‚B+‘
Psilocybe nataliensis
Panaeolous cyanescens ‚Hawai‘

The pics were shot on Marsh, 30th.
Used the following agar recipes:

1 liter water
20 grams light malt extract
20 grams agar

1 liter water
15 grams light malt extract
15 grams agar

I didn’t have enough time to post the pictures earlier.
Meanwhile the T2-plates are ready for the third transfer.
This will be done this sunday.


Psilocybe cubensis ‚Australia‘ – isolate ‚blue meanie‘:




Psilocybe cubensis ‚B+‘:




Psilocybe nataliensis:




Panaeolous cyanescens ‚Hawai‘:


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Re: Psychedelicmind's warm-up grow - trial and error! [Re: psychedelicmind] * 1
    #29198064 -

The following species keep looking sick on agar plates.
Will try to clean them up on water agar this weekend.


Panaeolous cyanescens ‚Jamaica‘:




Panaeolous tropicalis:




Psilocybe tampanensis:


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