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Re: Official Copelandia/Panaeolus Thread [Re: GoodGilbert] * 1
    #29104399 -

GoodGilbert said:
I need to make a 50th post...

There are many great threads on this site, but this one is probably my favorite. My panaeolus game is not strong, but it's getting much better. Everyone has been helpful. I appreciate all of you. Thanks


You're in the right place with a wonderful family of trusted peers. Take one day at a time, i'm still learning myself and i'm almost 50 lol :oldman:

Knowledge is a never ending journey :nyan:


--------------------
:ancientaliens:

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Re: Official Copelandia/Panaeolus Thread [Re: astra] * 8
    #29104569 -

Ahh thank you :heart:

And I feel the same :smile:



To contribute, here's some cyan bbar myc.

And Pan. Chlorocystis



--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: Official Copelandia/Panaeolus Thread [Re: tree frog] * 3
    #29105082 -

Thought I would share this.  I have been using seed starting trays and humidity domes for cubes and after a very successful TTBVI grow using gordotek that is still cranking out the goods, I decided to go ahead and send my backup plan into one of these seed trays with a dome just for the heck of it.

I like this setup for cubes because light is at least a part of the equation to trigger fruiting.  I know some folks swear that it isn't but I offer this picture of a 12qt clear box with no liner that bottom fruited like crazy.  I don't ever get this with these black trays.




Anyhow....It looks like a toothpick.  No man....Hey it is a toothpick.  No man....it's just.....It is a toothpick......Hold on man, I got the real stuff in the other pocket......

Here is the real stuff.

TTBVI Spores to agar.  Ended up going to 1% honey liquid culture off mother plate.  Did some transfers too, but they are still just sitting in a zip lock in a box.  Used pint mason jars with magnetic stir bar in bottom.  Stirred every few days until I had a lot of mycelium.  Transferred to bulk using sterile 60cc syringe with reusable 6 inch needles that I kept in boiling water on hot plate till ready to use.

Substrate is black cow/straw/vermiculite.  Put in mycobag and sterilized at 15psi for 3 hours.  Injected 100cc of LC directly into bag, sealed with hot glue and it sat on the floor in an open room exposed to light for a week or 2.  Was really not going to use it at all since other grow was going so good.  I shook it a couple times until it was completely colonized.  It sat a while longer....  Then dumped it in the pictured seed tray in a room with heat set on 75 degrees inside one of those cheap indoor greenhouses.  Let it recover for a few days.  Lid on, vents closed.

Cased with peat moss/vermiculite/pickling lime that was sterilized at 15psi for 2 hours.  Let it do its thing for a few days with vents open lid on.  When I started seeing some white working on the top, I set the lid ajar and started misting 2 times per day. 

This is about 10 days later.  No fancy set up but this worked great with TTBVI so far.  I like this better than the gordotek with the heater I think.  The greenhouse retains some heat and it is sitting not too far from a window so sunlight helps with heat too.  I do have curtains closed as I don't want to over heat.  The curtains are not opaque however and sun still heats and lights the room.

The other tray I have going like this is different genetics and is just now trying to pin in a couple places, but lagging behind quite a bit. 



--------------------
I may not have stayed at a Holiday Inn Express, but I have taken 20 grams of fresh subtropicalis :smile:

Edited by Hardhead (01/28/25 09:23 AM)

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Re: Official Copelandia/Panaeolus Thread [Re: Hardhead] * 1
    #29106067 -

Had bad luck with the 30:70 worm castings/coir shoeboxes. Two middling flushes and then nothing. Much lower yield than I’ve had with the same amount of spawn in larger monotubs. Got to full colonization in all shoeboxes. No mold or other weirdness that I could discern. Is that to be expected with shoeboxes, or are worm castings just not that great?

Just finished setting up some monos, two with the same substrate and another two with a similar substrate—but, I added some vermiculite, oyster shells, and gypsum to the mix to make it closer to what I’ve had success with already. No clue on if any of that actually help with anything, but it seems worth testing. Here’s hoping I can get the yield back up this round. If not, I guess I’ll go back to cow poo.

Casing at spawn vs casing at full colonization didn’t seem to make any difference, but it was a lot easier to watch what was going on with the uncased ones so I’m going to stick with casing later in the process from here on. So I got that figured out at least.

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Re: Official Copelandia/Panaeolus Thread [Re: Purrmes] * 17
    #29106136 -

Cute lil 3rd flush coming in.  Good timing for these guys to be done.  Got a blue spring qt colonizing fast. 


For anyone doing jcm chambers… not sure if you do this yet but I found it to be nice.  Bringing back an oldie.. the drip shield! :lol:  works pretty great actually.  I had this janky ass one made of plastic sheeting for the first couple flushes:


It worked for sure but had dips/wrinkles in it which would stop the drips from sliding down towards the bottom.  So I upgraded to plexiglass.  I even rain-x’d both sides.  Makes the chamber pretty maintenance-free



I’m going to drop the lower hooks another 1” or 1.5” to get an even steeper grade. 

And this might be useful to a select few but I have downstairs neighbors and when I put two bath towels folded once the long ways under the chamber, they absorb like 95% of the vibration from the two air pumps and very little is transferred to the floor.



I also used a bolt cutter to cut 2” off the two white wire tray legs to give the mush more headroom.  I looked forever for large trays that where short and couldn’t find anything but the cutters made quick work of that

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Re: Official Copelandia/Panaeolus Thread [Re: fahtster]
    #29106165 -

Just isolated my first pan cyan min culture from spore, any tips for a newbie to the strain or maybe a good pointer to a substrate tek/recipe?


--------------------
Check out my wish list and let me know what you got! UPDATED: 2/22/25
Current wish list


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Re: Official Copelandia/Panaeolus Thread [Re: mike-ologist] * 1
    #29106192 -

"Panaeolus" is the genus and "cyanescens" is the species :super:

Some guides on cultivating the species your working with:

Asura's guide on Panaeolus cyanescens cultivation. 

JOC TEK Panaeolus cyanescens

PANAEOLUS IN MONO TUBS 3.0

There is a few substrate recipes in the above.

I use screened and pasturised compost myself as a substrate addendment, 1 part grain, 1 part compost and 3 parts coir.

I think others use more compost and I might adjust those above ratios TBH.

Some people are also using liquid fertilizer, Mojave recipe.

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Re: Official Copelandia/Panaeolus Thread [Re: mike-ologist] * 3
    #29106193 -

Quote:
  I even rain-x’d both sides.  Makes the chamber pretty maintenance-free



Nice, I've been considering similar ideas lately. I haven't added a drip shield yet but I got some rain-x yesterday and applied it to the inside of the lid. Time will tell how well it works/lasts, but looks promising so far. I'll update with pictures of how it's doing in the morning.

Morning update-
So the treated lid is better than the two untreated lids. Smaller beads of condensation, and less of it. I still got a few drips when I moved the lid, but not nearly as much the others.
The drip guard is gonna be the better solution but rain-x does help.



--------------------
This hobby has taught me many things.
Most of those things are patience.

The Hitchhiker's Guide to the Shroomery
The Official Shroomery Cultivation Museum
CakeWars
III

Edited by Scemilope (01/29/25 07:36 AM)

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Re: Official Copelandia/Panaeolus Thread [Re: Dkeppel]
    #29106205 -

Dkeppel said:
"Panaeolus" is the genus and "cyanescens" is the species :super:

Some guides on cultivating the species your working with:

Asura's guide on Panaeolus cyanescens cultivation. 

JOC TEK Panaeolus cyanescens

PANAEOLUS IN MONO TUBS 3.0

There is a few substrate recipes in the above.

I use screened and pasturised compost myself as a substrate addendment, 1 part grain, 1 part compost and 3 parts coir.

I think others use more compost and I might adjust those above ratios TBH.

Some people are also using liquid fertilizer, Mojave recipe.


I'm just a lazy typer lol thanks for the info!


--------------------
Check out my wish list and let me know what you got! UPDATED: 2/22/25
Current wish list


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Re: Official Copelandia/Panaeolus Thread [Re: fahtster] * 1
    #29106208 -

fahtster said:
Cute lil 3rd flush coming in.  Good timing for these guys to be done.  Got a blue spring qt colonizing fast. 


For anyone doing jcm chambers… not sure if you do this yet but I found it to be nice.  Bringing back an oldie.. the drip shield! :lol:  works pretty great actually.  I had this janky ass one made of plastic sheeting for the first couple flushes:


It worked for sure but had dips/wrinkles in it which would stop the drips from sliding down towards the bottom.  So I upgraded to plexiglass.  I even rain-x’d both sides.  Makes the chamber pretty maintenance-free



I’m going to drop the lower hooks another 1” or 1.5” to get an even steeper grade. 

And this might be useful to a select few but I have downstairs neighbors and when I put two bath towels folded once the long ways under the chamber, they absorb like 95% of the vibration from the two air pumps and very little is transferred to the floor.



I also used a bolt cutter to cut 2” off the two white wire tray legs to give the mush more headroom.  I looked forever for large trays that where short and couldn’t find anything but the cutters made quick work of that



JCM user here. Loving your drip shield Faht..OG AF :doublejointed::heart::heart:

ps,
My jcm is posted on my journal, nothing extraordinary but does the job :mushroom2:

Edited by astra (01/29/25 04:30 AM)

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Re: Official Copelandia/Panaeolus Thread [Re: astra] * 11
    #29106239 -

Going to have to give the jcm a go soon but tubs are just too damned convenient :shrug:
Do, errr, Hausteca drop spores?
Got a few more tubs going with the fresh hpoo substrate that’s been doing strange things and this tub is no exception, every day I think they’re about to drop and I can harvest and every day they get bigger, caps on the more mature ones are averaging 3 inches across and suffering from constant misting and evaporation, I’m just keeping the lid on now to maintain humidity but these things are getting massive!


Might just harvest in the morning anyway before they get too manky :/


--------------------

Clean MS from fresh fruits every time.
How I prep brown rice

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Re: Official Copelandia/Panaeolus Thread [Re: 3.A.M]
    #29106248 -

Hi all,

Is this a contamination or a good mycelium of Copelandia florida

https://www.shroomery.org/forums/thumbs/25-005/798814327-thumb_IMG_4156.jpg

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Re: Official Copelandia/Panaeolus Thread [Re: MedicinalMushroom]
    #29106249 -

When I look to the lines on the mycelium grow I think it is a contamination because Penicillium has a same growing method. But I have no id how copelandia grow

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Re: Official Copelandia/Panaeolus Thread [Re: Dkeppel] * 4
    #29106424 -

Dkeppel said:
"Panaeolus" is the genus and "cyanescens" is the species :super:

Some guides on cultivating the species your working with:

Asura's guide on Panaeolus cyanescens cultivation. 

JOC TEK Panaeolus cyanescens

PANAEOLUS IN MONO TUBS 3.0

There is a few substrate recipes in the above.

I use screened and pasturised compost myself as a substrate addendment, 1 part grain, 1 part compost and 3 parts coir.

I think others use more compost and I might adjust those above ratios TBH.

Some people are also using liquid fertilizer, Mojave recipe.


FYI, if you read my write up a long time ago, it is no longer the long, janky mess it used to be :laugh:

Everything was rewritten from the ground up, reorganized, simplified, shortened etc.

I have added a spawn to bulk method in addition to the LC method in the OP.


--------------------
Cultivating Panaeolus cyanescens


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Re: Official Copelandia/Panaeolus Thread [Re: Asura]
    #29106431 -

Awesome, I'm excited to play with this species :smile: bucket pasteurizing tek should work with the sub recipes right?


--------------------
Check out my wish list and let me know what you got! UPDATED: 2/22/25
Current wish list


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Re: Official Copelandia/Panaeolus Thread [Re: mike-ologist] * 2
    #29106435 -

Proper pasteurization is preferred.


--------------------
Cultivating Panaeolus cyanescens


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Re: Official Copelandia/Panaeolus Thread [Re: Asura]
    #29106466 -

Asura said:
Proper pasteurization is preferred.



Agreed. Good advice, especially with manure/compost based bulk substrates.

I find myself preferring the 'sous-vide' style of pasteurization for smaller amounts of bulk.

Not that anyone asked but here it is anyway for the curious inclined 😏😎.

Fill a gallon freezer ziplock bag with field capacity hydrated bulk material, compress it as flat as you can (getting excess air out) and weigh it down in a pot. I use a trivet or jar lids to keep it from touching the bottom of the pot for obvious reasons. Heat up the water to 140-160° F for an hour or so. The center of the substrate, being flattened out and within a thin bag (unlike when done in jars) is the same temp as the water within minutes so you only have to temp the water with a meat thermometer. I find that it even 'alerts' you when it reaches 140°, it will start to float, like a boss. 😆 That's really the only tricky part, keeping it submerged... but you can use your imagination and use a plate with a brick or similar. You're no dummies, you'll find ingenious ways. 👍🏻

Less messy, cools faster, ready to go, no clean up. Just a suggestion. -shrug-


--------------------
Chock full o' nuts. ☕

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Re: Official Copelandia/Panaeolus Thread [Re: ShitdickNancy] * 1
    #29106522 -

I like sous vide too. And yeah it can be a little goofy.

https://www.shroomery.org/forums/showflat.php/Number/29101668

It seems like things just work out better when I do it.


--------------------
Cultivating Panaeolus cyanescens


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Re: Official Copelandia/Panaeolus Thread [Re: Asura]
    #29106537 -

Asura said:
I like sous vide too. And yeah it can be a little goofy.

https://www.shroomery.org/forums/showflat.php/Number/29101668

It seems like things just work out better when I do it.



❤️

Well, that was a surprise synchronicity. 😅

I didn't realize you recently did a write up on it, my bad Asura.

Thank you for putting it out there for newer cultivators! 🙌🏻 TC word travels far & wide.

Even better with the freezer saver bags! Less chance of leaking etc.


--------------------
Chock full o' nuts. ☕

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Re: Official Copelandia/Panaeolus Thread [Re: ShitdickNancy]
    #29106543 -

Also admire your Ten 🐂 bulls avatar! 🙌🏻

Thank you for that; been quite awhile since I've revisited those poems/illustrations. Made my day. 😊


--------------------
Chock full o' nuts. ☕

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Shop: Mushroom-Hut Liquid Cultures   North Spore Bulk Substrate   Myyco.com Golden Teacher Liquid Culture For Sale   MagicBag.co All-In-One Bags That Don't Suck   Sporeworks.EU Spores for European Microscopy   Original Sensible Seeds Autoflowering Cannabis Seeds

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