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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Pristine BRF Puck tek [Re: Baba Yaga] * 3
    #28799322 -

Thanks, I have been meaning to make a post about it. Finally did.


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Stoned Gummys | BRF Pucks | Primo Reusable Plates | Easy LI 4 Preserv & Propo
"Psychedelics are powerful substances. Nothing that powerful is completely safe... and nothing completely safe is that powerful!" - Abigail Calder at ALPS 2023
Don't Panic

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Re: Pristine BRF Puck tek [Re: ReverendMyc] * 5
    #28810906 -

Got some bisprous prints that I really wanted to get going. So each got two sterile swabs dipped in sterile water and a tiny rub on the print then jammed into the side of a puck. I like how they are looking after a week.



Also some clone tissue from a few other lines that I want to lock down two weeks in.
Nats, Jack Frost & Tamps.
The little arrows indicate where I placed the tissue.

I like setting the inoculant to one side and then taking transfers from the leading edge when it reaches half way across the puck. Then close it back up and hope for pins or eventually top fruit them in a water tub.


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Stoned Gummys | BRF Pucks | Primo Reusable Plates | Easy LI 4 Preserv & Propo
"Psychedelics are powerful substances. Nothing that powerful is completely safe... and nothing completely safe is that powerful!" - Abigail Calder at ALPS 2023
Don't Panic

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Re: Pristine BRF Puck tek [Re: ReverendMyc] * 1
    #28815345 -



A few BRF pucks have fully dried up after full colonization. Dropping this into sterile water or LC broth should bring it back to life or putting some crumbs onto agar might do too. I mean it does work with dried grains so no reason this shouldn't work. It would be easier to break up the puck once fully colonized but still soft before letting it dry out cause these puck can get quite hard.

Will give it a couple more month before trying this out.


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🍄PANAEOLUS IN MONOTUBS🍄
🍄OFFICIAL SEMILANCEATA THREAD🍄



⭐Baba's Write Ups and Link Collection⭐

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Re: Pristine BRF Puck tek [Re: Baba Yaga]
    #28816191 -

That is a really cool idea. I can't see why it wouldn't work.

My glass jars take a really long time to dry out, though. At least pucks that I had thought were dried were still pretty squeezable when I went to clean the jars to reuse. But now you have me wondering about putting colonized pucks into Ziploc bags to store and continue to dry out. Maybe a lunchbox cooler in the fridge full of labelled baggies like agar plates or slants? Worth exploring for sure.

Thanks Baba!


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Stoned Gummys | BRF Pucks | Primo Reusable Plates | Easy LI 4 Preserv & Propo
"Psychedelics are powerful substances. Nothing that powerful is completely safe... and nothing completely safe is that powerful!" - Abigail Calder at ALPS 2023
Don't Panic

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Re: Pristine BRF Puck tek [Re: ReverendMyc] * 1
    #28816639 -

I still want to try the filter paper method where you punt a piece of slightly moist, sterilized filter paper onto agar and place a transfer next to it. Once colonized remove the paper and let it dry. This method was discussed as a suitable low cost storage to use in third world countries.

Two days ago I got rid of all, 30 or so, cultures I had in my fridge. It's all LC and dried BRF Pucks at room temp now.


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🍄PANAEOLUS IN MONOTUBS🍄
🍄OFFICIAL SEMILANCEATA THREAD🍄



⭐Baba's Write Ups and Link Collection⭐

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Re: Pristine BRF Puck tek [Re: Baba Yaga] * 1
    #28816681 -

The selected post/topic has been added to your favorites....

Nice write up and clear pics!  Great job!


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🅃🄴🄰🄼 🄸🄷🄾🄿


New to cultivation or the shroomery?  Observe the hitchhiker's guide

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Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

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Re: Pristine BRF Puck tek [Re: ReverendMyc]
    #28850017 -

Thanks for the thread. Quick question -

Once the puck is showing growth and you want to transfer something from the puck jar back to agar, what is the process? I understand if it's pinning you could just grab a pin, but if not, what are you moving to agar? Trying to grab a bit of just fluffy myc from the top? A little scoop of BRF mixture showing growth on it? Any photos of agar dishes you've recently transferred to from these pucks to see material / size before growth overtakes it?

Thanks in advance. Currently struggling to get a spore syringe to germ on agar and would like to try this method.

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Re: Pristine BRF Puck tek [Re: strangemyc] * 1
    #28850484 -

This should be ideal for a stubborn syringe.
I try for just some mycelium fluff, but often get a bit of the puck riding along. No problem either way.


1688 to 1696 are all transfers from pucks. It is a mix of pans, cubes, subtrops, and mexicana. So the growth could be a bit misleading. The 1689 Bisporus is totally mold, so don't reference that one.

I take the smallest piece that I can, and sometimes they are a little hard to get off of the scalpel tip. Using two scalpels is a trick I learned from Smelly Hobbit for that.

1688 2024-03-20 Plate celtreat 2024-04-02 Pan Cambo Komodo
1689 2024-03-20 Plate celtreat 2024-04-02 Pan bisporus
1690 2024-03-20 Plate celtreat 2024-04-02 Subtrp Bell
1691 2024-03-20 Plate celtreat 2024-04-02 Subtrp Bell
1692 2024-03-20 Plate celtreat 2024-04-02 GT
1693 2024-03-20 Plate celtreat 2024-04-02 Mex CN
1694 2024-03-20 Plate celtreat 2024-04-02 Pan Cyan BVI
1695 2024-03-20 Plate celtreat 2024-04-02 Pan Cyan BVI
1696 2024-03-20 Plate celtreat 2024-04-02 Pan Cyan Brooks


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Stoned Gummys | BRF Pucks | Primo Reusable Plates | Easy LI 4 Preserv & Propo
"Psychedelics are powerful substances. Nothing that powerful is completely safe... and nothing completely safe is that powerful!" - Abigail Calder at ALPS 2023
Don't Panic

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Re: Pristine BRF Puck tek [Re: ReverendMyc]
    #28850621 -

Thanks for the quick response. Looking forward to trying this out.

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Re: Pristine BRF Puck tek [Re: ReverendMyc] * 3
    #28873128 -

Did this with a ghost swab and a few others. This is a piece of a pin (came apart when I tried to pluck it with tweezers) from the brf puck that later grew this pin out of that, so brf puck pin clone pin lol

This stuff is a game changer for stubborn or dirty cultures. Thanks again, Rev!



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Re: Pristine BRF Puck tek [Re: Hysteria] * 1
    #28989776 -

I was looking for a way to get some stubborn swabs to germ, and came across this technique.  I thought the jars were a bit large just for getting germination, so I just made thin pucks in my reusable petris. It worked fairly well, but they seem to dry out quick. I just made some transfers so we'll see how that goes.
Thank you for posting this Rev!

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Re: Pristine BRF Puck tek [Re: bambus] * 2
    #29019394 -

Sweet thread, Rev!  Since getting a FFU, I’ve debated over just using these pucks or going agar.  Agar just seems like so much more work/materials lol so I’m leaning towards these.  I did do a search and didn’t see anything but I was wondering if you had tried trenching with these?

It seems like it would be an ideal media to use it with.  The depth is better so the worry of bacteria moving all the way from the bottom of one side to the top of the other before myc reaches over would be less.  The trenches would be way more easily made (brf mix sticks together easily).  I plan on giving it a shot here today.  I’m going to just make the trench with a butter knife pushing against the side the myc is reaching to as to not compact the other.

Thoughts?

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Re: Pristine BRF Puck tek [Re: fahtster]
    #29019437 -

I'm sure I read Josex on his older brf puck thread saying he stopped using agar and switched to 100% BRF pucks for a while because they are a bit slower so he could reduce his workload with the benefits of being cheaper and contam resistant.

This trenching thing sounds interesting so I'm
:threadmonitor:

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Re: Pristine BRF Puck tek [Re: Radamanto]
    #29019483 -

:threadmonitor:


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Learn it, know it, live it


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Re: Pristine BRF Puck tek [Re: Mackload] * 7
    #29019522 -

Aight.. giving it a shot.  The trenches were very easy to make.  Did use a butter knife.  I have two cultures that are obviously bacterial so we’ll see how it goes and I’ll post the results

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Re: Pristine BRF Puck tek [Re: fahtster] * 2
    #29019554 -

Oh cool. That is not how I pictured trenching but it looks like the way to go. Obviously I haven't tried it yet but I will be very interested to see how it goes.

I was thinking just a wedge shaped trench on the surface like with agar and didn't see how that would make much impact in the puck since myc could just burrow under. That gap makes so much sense. Maybe I didn't understand how the trench work in agar either.


--------------------
Stoned Gummys | BRF Pucks | Primo Reusable Plates | Easy LI 4 Preserv & Propo
"Psychedelics are powerful substances. Nothing that powerful is completely safe... and nothing completely safe is that powerful!" - Abigail Calder at ALPS 2023
Don't Panic

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Re: Pristine BRF Puck tek [Re: ReverendMyc]
    #29019566 -

sort of related - do people use tools other than scalpels for transfers from pucks? maybe butter knives? I struggled with a scalpel - maybe cutting too deep, and used a sterilized teaspoon in the end, scooping some grains too. but for finesse is scalpel and careful cutting still your best bet?


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Learn it, know it, live it


Edited by Mackload (11/10/24 05:27 PM)

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Re: Pristine BRF Puck tek [Re: Mackload] * 1
    #29019584 -

You could try a needle and josex poke some of the myc in the puck to transfer to agar. That should work to get a clean culture from the puck

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Re: Pristine BRF Puck tek [Re: Mackload] * 1
    #29019684 -

Mackload said:
sort of related - do people use tools other than scalpels for transfers from pucks? maybe butter knives? I struggled with a scalpel - maybe cutting too deep, and used a sterilized teaspoon in the end, scooping some grains too. but for finesse is scalpel and careful cutting still your best bet?


I just took a small hunk of substrate with the transfer and haven't had an issue, if thats the issue you're running into.


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Hitchhikers Guide to The Shroomery

Shine on forever, shine on benevolent son
Shine down upon the severed
Shine until the two become one

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Re: Pristine BRF Puck tek [Re: fahtster]
    #29019786 -

fahtster said:
Aight.. giving it a shot.  The trenches were very easy to make.  Did use a butter knife.  I have two cultures that are obviously bacterial so we’ll see how it goes and I’ll post the results



:popcorn:


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All I need are some tasty waves, a cool buzz, and I’m fine.
Whatever you decide won’t really impact our survival
Close your eyes, and do the best that you can

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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