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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Psychedelicmind's warm-up grow - trial and error!
    #28958787 -

Hey guys,

yesterday i started my first grow after a few years of break.
In those days i only grew cubes. I didn't use agar or a flow hood. Just inoculated grain jars with spore syringes, cased my cultures and let them fruit. The first year i got some nice yields but after a while i kept having contamination problems. Frustation spread out and i finally stopped my hobby because i didn't have enough free time to identify and eliminate the sources of errors.

Now i want to start again. This time with more professional equipment and more patience.
I decided to post my cultivation work in this grow log.
I would appreciate the help of more experienced growers.
This board is much more informative than any book about mushroom growing i have read.

Mush love.

Psychedelicmind

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Re: Psychedelicmind's warm-up grow - trial and error! [Re: psychedelicmind]
    #28958813 -

The first thing i got going is a new incubator.
I used a new polystyrene box and equiped it with an heating cable and a reptile thermostat.
The cable was fixed with bent nails.
The openings for the cable were sealed with silicone afterwards.
I set the temperature to 28° C (82.4° F), switching delay 0.5° C.
The problem is that the temperature is going up and down from 26.2° C (79.2° F) to 29.8° C (85.6° F)
What is the best way to fix that?
The switching delay can't be reduced, so i got the idea of using a dimmer to reduce the heat of the cable or to pull half of the cable out of the box. Is there another way to keep the temperature more constant?

Thanks in advance!


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Re: Psychedelicmind's warm-up grow - trial and error! [Re: psychedelicmind]
    #28958878 -

Hi. Not sure why you’re making that

I liked your flow hood and agar talk. Those two will really help in making successful grows. The flow hood makes life easier and agar makes it all visible. Walking that path sounds like a win and you’ll be able to nail those contamination issues

On another path, have you read the HHG (link in my sig)? PF Tek may be a soft re-entry as well. It’s pretty much foolproof in my experience

Happy growing, PM


--------------------
Hitchhiker’s Guide to the Shroomery “Clean spawn is easily the most critical component of the entire cultivation process.”
Drink the Water of Life and become Paul Muad’Dib.
Remember to surrender.

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Re: Psychedelicmind's warm-up grow - trial and error! [Re: AzureCultivator]
    #28959638 -

Well one thing is tub in tub incubator is the best way. Second your jars and such will colonize fine at 50f or above. Welcome back .


--------------------
The better guide to grow mushrooms.
https://www.shroomery.org/forums/showflat.php/Number/8553541

Beware the power of one.  The power of two. The power of many.

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Re: Psychedelicmind's warm-up grow - trial and error! [Re: vicepope]
    #28961225 -

Thanks for your advice, guys!

@AzureCultivator:

Yeah i hope so. HI have never worked with a flow hood before. Tried to do some agar work in the past in a SAB, but failed. :/
Started my new flow hood last sunday, will post a few pics later.

I have not read the HHG until now, but i saved the link already and will read it this weekend. :cool:

@vicepope: That sounds good. I will use the search to find a construction tutorial.

Are temperature fluctuation during incubation a problem for species like pan. cyan. and sclerotiaproducers?

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Re: Psychedelicmind's warm-up grow - trial and error! [Re: psychedelicmind]
    #28961248 -

Some pics of my flood hood.
The setup was quite easy.
The only problem is that i discovered some gaps around the hepa filter:

Gaps between the left and right side elements of the box (the black ones, not the transparent ones) and the white frame of the hepa filter.
The side elements seem to be bent a little bit.

A big gap at the left side between the hepa filter and its white frame. Probably the glue has dissolved.

Do you think i should fix it with silicone or acryl?
It should not be persistent of course, because i will need to replace the filter over the course of time.

 

Edited by psychedelicmind (09/25/24 12:33 PM)

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Re: Psychedelicmind's warm-up grow - trial and error! [Re: psychedelicmind]
    #28961270 -

So this flow hood, can you go into more detail about it?


--------------------
🅃🄴🄰🄼 🄸🄷🄾🄿


New to cultivation or the shroomery?  Observe the hitchhiker's guide

The Hitchhiker's Guide to the Shroomery



Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

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Re: Psychedelicmind's warm-up grow - trial and error! [Re: psychedelicmind]
    #28961272 -

psychedelicmind said:
Some pics of my flood hood.
The setup was quite easy.
The only problem is that i discovered some gaps around the hepa filter:

Gaps between the left and right side elements of the box (the black ones, not the transparent ones) and the white frame of the hepa filter.
The side elements seem to be bent a little bit.

A big gap at the left side between the hepa filter and its white frame. Probably the glue has dissolved.

Do you think i should fix it with silicone or acryl?
It should not be persistent of course, because i will need to replace the filter over the course of time.

 


Welcome back to the cult!

Regarding your flow-hood, it very much resembles the same piece of kit that I've bought when I got back into growing. I hope you won't have the same disappointment that I had, and find that it doesn't have a true laminar flow, and that it actually creates quite a bit of turbulence. Search youtube for laminar flow hood test, you can do it with an incense stick by observing the flow of smoke. I've actually used it twice before I made the test, and got terrible contamination. I really hope that yours is better, as mine is up in the loft now, and the worst £280 ever spent.

As the others said, you really don't need an incubator, room temp is fine for most grows, even pans. Tub in Tub is an easy system, just smaller plastic trays in a bigger plastic Tub, you don't need to build anything, just buy some tupperware from any store. They more or less create and maintain their own climate with the right adjustments.

The thing that I wanted to tell you the most is please, please, stop buying things before you research all the dedicated topics for whatever you want to grow.
I say this from experience, as I had a long break, started again by buying stuff, and researched after. I've spent so much money on useless things that I don't even want to add it up. I just want to spare other people.

Have a great day reading :wink:

Edited by hipopotam (09/25/24 12:55 PM)

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Re: Psychedelicmind's warm-up grow - trial and error! [Re: hipopotam]
    #28961308 -

@Mr. Piggy: I imported the flow hood from an UK vendor, price was about 520 EUR + 110 EUR custom fees. I am not sure if it is allowed to post the name of the vendor here?

@hipopotam: Yeah, i think i have already bought too many things, thinking that this perfectionism would solve my former contam problems.
Thanks for the advice with the incense stick, i will try that.
Tried to check the performance of my flow hood with a lighter. The flame shows an about 45 degree angle, but in the middle of the filter. In the lower areas of the filter, there is a little bit less air flow i suppose. Nevertheless i inoculated five petris with a pan. cyan. sporesyringe and until now they seem to be clean. Will post some pics soon... I opened another petri in my living room just to see the difference. It was mold-contaminated after one day. The ones i opended before my flow hood, seem to be okay, but i have no experience until now...

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Re: Psychedelicmind's warm-up grow - trial and error! [Re: psychedelicmind]
    #28964881 -

This is the result of last sunday.
I inoculated 5 petri dishes and 4 pf-tek jars with a Panaeolous cyanescens sporesyringe.

Is was the last syringe the shop had, so i think it might me a litte bit too old.

Until now i cannot see any germination of the spores.

I have bought these the pre-poured plates (MEA) from an online vendor.
They came perfectly sealed without any condensation on the tops.
After inoculation with one drop of spore solution and putting them into my incubator there is so much condensation on the tops that it is impossible to see if there is any growth yet.

Is there an easy way to get rid of the condensation now?

My thought was to remove the parafilm in front of my flow hood and let the dishes rest there without opening them.

Do you have any other ideas?

Have a nice weekend.

psychedelicmind


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Re: Psychedelicmind's warm-up grow - trial and error! [Re: psychedelicmind]
    #28964992 -

Just opened my petris in front of my flow hood.
The spores were inoculated to the centre of the dishes.
I cannot see any germination until now.
The areas near the edges look strange to me.
Is the agar drying out there or is it a kind of contam?


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Re: Psychedelicmind's warm-up grow - trial and error! [Re: psychedelicmind]
    #28990600 -

Here are some pics of my petri dishes, pan. cyan. Jamaica and pan. tropicalis.

Some of them did not show any sign of germination, so i gave them a second shot of spores.

The mycelium looks quite strange i think. Neither whisby and nor fluffy at all.

Agar: Kimmig agar, bought ready to use from a shop.

They were incubated at 27-28 °C. (80.6-82.4 °F)

Poured some MEA plates and want to do tranfers to those tomorrow.
Not sure if my plates are contaminated, don't want to spread mold spores opening the plates infront of my flow hood.

Would appreciate your feedback.


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Re: Psychedelicmind's warm-up grow - trial and error! [Re: psychedelicmind] * 1
    #29094455 -

REBOOT

after a lot of failure i decided to complete reboot my project.
I used the time to read a lot on shroomery and to watch RR's mushroomvideos.

Got a new and properly working flow hood now.
An i got some practice in pouring, handling and sealing agar plates.

The following species are on agar right now:

Psilocybe cubensis varity Wollongong (3rd transfer done, looking clean)
Psilocybe cubensis varity Fidgi (germination plates, looking white and fluffy)
Psilocybe cubensis varity Puerto Rico (germination plates, looking white and fluffy)
Psilocybe cyanescens (3rd transfer done, looking clean)
Psilocybe tampanensis (germination plates)
Panaeolous cyanescens varity Jamaica (germination plates)
Panaeolous tropicalis (germination plates)

Will post some pics of each strain on agar next weekend when i am back at home.

Also made some LC from Psilocybe cyanescens und Cubensis Wollongong.
Pics will follow...

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Re: Psychedelicmind's warm-up grow - trial and error! [Re: psychedelicmind] * 1
    #29094999 -

You aim to keep busy...


--------------------
Agar Plates and Removing Hidden Contaminants - A Visual Notebook
Recognizing Contamination in Grain Jars - A Visual Notebook
Contaminated Condiment Cup Cleanup v1.0

Green Cap Pheno Isolation
🅃🄴🄰🄼 ​ 🄲🄾🄽🄳🄸🄼🄴🄽🅃 ​ 🄲🅄🄿

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Re: Psychedelicmind's warm-up grow - trial and error! [Re: DV8DUG]
    #29102328 -

So, now i finally got the time to post an update of my grow.
Yesterday i poured 60 plates.
Used the following recipe:

15 grams MEA / Liter
20 grams Agar / Liter
1 gram Peptone / Liter

PCed 20 minutes at 15 psi with my Presto 23 quart PC.
Started to pour at 44 degree Celsius (about 111.2 degree Fahrenheit)
After cooling down i sealed them with Parafilm, put them into ziplocks and put them into the fridge.

 

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Re: Psychedelicmind's warm-up grow - trial and error! [Re: psychedelicmind]
    #29102333 -

Here is an update of my Psilocybe cyanescens.
I ordered an mycelium syringe and inoculated a few plates.
The pictures show the second transfer.
In my opinion the mycelium looks healthy, but i don't have any experience with this species yet.
Would really appreciate your feedback.
My plan is to do a few more transfers and then use the dishes to inoculate a wood-based substrate.
Want to use the substrate for an outdoor grow.



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Re: Psychedelicmind's warm-up grow - trial and error! [Re: psychedelicmind]
    #29102351 -

Update of Psilocybe cubensis var. Fidgi:
First transfer was done on January, 10th.
The pictures were shot on January, 21st.
Want to to a few more transfers and then use the agar to make LC.
The filled and sterilized LC jars are sitting in my fridge, ready for use.


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Re: Psychedelicmind's warm-up grow - trial and error! [Re: psychedelicmind] * 1
    #29102355 -

Update of Psilocybe cubensis var. Puerto Rico:
First transfer was done on January, 10th.
The pictures were shot on January, 21st.
Want to to a few more transfers and then use the agar to make LC.
The filled and sterilized LC jars are sitting in my fridge, ready for use.


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Re: Psychedelicmind's warm-up grow - trial and error! [Re: psychedelicmind]
    #29102356 -

Var Puerto rico!? What is that one! Is it just like a regular gt that came from PR?  I'm half Puerto rican and visit Puerto rico once every few years so id love to grow a cubensis from PR!


--------------------
Endlessly endorsing empathy empirically energizing existence™

~Always practice radical kindness~
R.I.P. Betty Boop, greatest mother to ever do it.

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Re: Psychedelicmind's warm-up grow - trial and error! [Re: QuantumZ00]
    #29102403 -

QuantumZ00 said:
Var Puerto rico!? What is that one! Is it just like a regular gt that came from PR?  I'm half Puerto rican and visit Puerto rico once every few years so id love to grow a cubensis from PR!


Hey QuantumZ00,

i bought this print from an online store, made a syringe and put it onto agar.
If i will be successful in growing them out, i can sent you a print if you want.
The print was declared as "Psilocybe cubensis - Varity Puerto Rico".
Seems to grow wild there?  :smile:

Edited by psychedelicmind (01/26/25 09:09 AM)

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