Phaethon said:

Definitely not trichoderma; I don't think there is any mold of any sort. Get some other opinions, but as far as I can see through that lid, you have a healthy germination plate. Take a few transfers - you can do 2 or 3 per donor plate if you want to conserve plates while having more to choose from.

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chummmp said:

I tried to take on board the very valid criticisms of my previous pictures and improve, but I still think they're not ideal.  Really messed with lighting and phone settings but I guess my resources (and brain) are limited.  Hope this is a little better and has a little more clarity.  Probably just a bunch of trich, although these are about 12 days at approx 21c and none of them are showing green so maybe not? 

Again, thanks for the replies, thoughts and time you've given me.  Cheers.

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These are germination plates, and 12 days old

I thought these were first transfers, and I was seeing satellite colonies.

Sorry my man, I didn't mean to freak you out.

The growth will look more organised with transfers.

Bigdogg said:

chummmp said:

I tried to take on board the very valid criticisms of my previous pictures and improve, but I still think they're not ideal.  Really messed with lighting and phone settings but I guess my resources (and brain) are limited.  Hope this is a little better and has a little more clarity.  Probably just a bunch of trich, although these are about 12 days at approx 21c and none of them are showing green so maybe not? 

Again, thanks for the replies, thoughts and time you've given me.  Cheers.

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Still not great but much better.

These 2 spots look good. Don't transfer the whole area I marked. Just small slices at the edge of the myc growth. 




They're at likely more spots to transfer. My eyes are getting old but I can see those 2 breakout spots. You want to look for the germination points and go for the mycelium that is growing fastest away from it. Any growth that turns rhizo get that as well. Here is the germination point of one section I marked and an outline of the myc growing away from it.

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Dkeppel said:

These are germination plates, and 12 days old

I thought these were first transfers, and I was seeing satellite colonies.

Sorry my man, I didn't mean to freak you out.

The growth will look more organised with transfers.

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chummmp said:

Bigdogg said:

chummmp said:

I tried to take on board the very valid criticisms of my previous pictures and improve, but I still think they're not ideal.  Really messed with lighting and phone settings but I guess my resources (and brain) are limited.  Hope this is a little better and has a little more clarity.  Probably just a bunch of trich, although these are about 12 days at approx 21c and none of them are showing green so maybe not? 

Again, thanks for the replies, thoughts and time you've given me.  Cheers.

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Still not great but much better.

These 2 spots look good. Don't transfer the whole area I marked. Just small slices at the edge of the myc growth. 




They're at likely more spots to transfer. My eyes are getting old but I can see those 2 breakout spots. You want to look for the germination points and go for the mycelium that is growing fastest away from it. Any growth that turns rhizo get that as well. Here is the germination point of one section I marked and an outline of the myc growing away from it.

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Can't thank you enough for the time you took to mark this out for me and provide this info! 

If I may trouble you again, is the germination point the point at which the spores from the syringe first began forming hyphae and exapnding outwards?  Is the growth moving fastest away from it therefore 'stronger' or 'mature' or somesuch?  Just trying to understand the process more deeply.  Currently reading "an entangled life" and it's all so interesting!
 
Thanks everyone for helping me out, going to make some plates up tonight and make some transfers.  Appreciate you all.

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nektar61 said:

PBJ710 said:

X7X+2

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This tight rhizo looks right and like many that I had, I'd transfer or send from this.

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Got some slants getting their lean on and once they are cooled, I'll start archiving stuff.  I went ahead and poured another stack of plates while I was making agar since it'll get used in short order.



For those slant tubes, should I leave the lids slightly cracked for GE and wrap them with parafilm or just cinch them down tight and not worry about any air?

Bigdogg said:

inski said:

What species is this?

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Those are all the same species?

Top center looks like Weraroa.

Looks like some Subtropicalis, Semilanceata, Caerulescens.

All the same or a game to be each pic?

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Dkeppel said:

You got covid while on holiday? That sucks!

Thanks man, I wish you a speedy recovery too, and clean plates on return.

Pic tax, I just dropped this plate on the floor

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DcevLearn said:

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inski said:

Dkeppel said:

You got covid while on holiday? That sucks!

Thanks man, I wish you a speedy recovery too, and clean plates on return.

Pic tax, I just dropped this plate on the floor

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Hi Dkeppel, what species is that you have on that plate?

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ThurmanMurman said:

PE germ plate. This would be the first I've had anything on a germ plate that wasn't obviously a contaminate, but something seems off about it. Im considering the possibility that the first growth from germination may just look kinda funny, but idk...it doesnt sit right with me. Sorry for the crappy pic - it was a chore just to snap one this good, due to the condensation.

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