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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Post Your New Tools Of The Trade. [Re: PhenoDreamers]
    #28679967 -

Do you wrap them after use?


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halfass mycology

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Re: Post Your New Tools Of The Trade. [Re: GenericHero]
    #28679980 -

GenericHero said:
Do you wrap them after use?



In half ass mycology we just rubber band it and call it a day

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Re: Post Your New Tools Of The Trade. [Re: mycoheadonaut] * 2
    #28680001 -

I spore print in large Tupperware. I have clean prints and swabs but by all accounts I’m pretty lax with them. I package swabs in open air and I’ve never had a swab I made in-house be dirty.


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A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: Post Your New Tools Of The Trade. [Re: mycoheadonaut]
    #28680119 -

mycoheadonaut said:
GenericHero said:
Do you wrap them after use?



In half ass mycology we just rubber band it and call it a day


I have tried going this route. Couldn't find just the right size bands.

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Re: Post Your New Tools Of The Trade. [Re: Yahra] * 2
    #28680392 -

Yahra said:
Ah, ok, now i understand.

There are several cases i can think of, for example spore printing or leaving poured petri dishes unwrapped in the SAB until you use them.


The only time I covered the holes of my SAB was when I had hot pours cooling overnight. I just threw a t-shirt over it and anchored it with something heavy enough to keep it in place.

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Re: Post Your New Tools Of The Trade. [Re: Yahra]
    #28680978 -

Yahra said:
Ah, ok, now i understand.

There are several cases i can think of, for example spore printing or leaving poured petri dishes unwrapped in the SAB until you use them.


So this still doesn't make sense to me.  if you have a properly working SAB then you shouldn't have to cover the arm holes, just don't mess with it.  Pulling covers off of the holes will create a minor vacuum which will draw a lot of air and microbes in.  it doesn't matter if they are screw on, magnetic, or taped.  :shrug:


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🅃🄴🄰🄼 🄸🄷🄾🄿


New to cultivation or the shroomery?  Observe the hitchhiker's guide

The Hitchhiker's Guide to the Shroomery



Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

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Re: Post Your New Tools Of The Trade. [Re: Mr Piggy]
    #28681033 -

I am not a physicist, but "create a minor vacuum which will draw a lot of air and microbes in." sounds like an exaggeration to me.

The covers don't seal the SAB shut, there are little gaps all over the place.

Frontal view:


Top down view:


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:heart: 🅃 🄴 🄰 🄼   🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿 :heart:


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Re: Post Your New Tools Of The Trade. [Re: Yahra] * 1
    #28681075 -

When a seal is pulled away, it creates minor suction and it disturbs air.  Put a lit candle in the box in front of the arm holes, put the covers on, then pull them off again.  Guaranteed that little flame will dance.  Same principle as why we don't use glove boxes and instead leave the arm holes open. 

I'm not trying to stop you from doing your thing, I just don't agree with the general principle and think it would be minorly detrimental vs helpful.


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🅃🄴🄰🄼 🄸🄷🄾🄿


New to cultivation or the shroomery?  Observe the hitchhiker's guide

The Hitchhiker's Guide to the Shroomery



Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

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Re: Post Your New Tools Of The Trade. [Re: Mr Piggy] * 1
    #28681081 -

Maybe just don't rip the covers off as fast as you can


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halfass mycology

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Re: Post Your New Tools Of The Trade. [Re: GenericHero]
    #28681095 -

It's still creating a disturbance and negating the entire point of them being there, speed doesn't matter.


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🅃🄴🄰🄼 🄸🄷🄾🄿


New to cultivation or the shroomery?  Observe the hitchhiker's guide

The Hitchhiker's Guide to the Shroomery



Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

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Re: Post Your New Tools Of The Trade. [Re: Mr Piggy]
    #28681102 -

I think you're wrong about that


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halfass mycology

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Re: Post Your New Tools Of The Trade. [Re: GenericHero]
    #28681129 -

Care to elaborate on what you disagree with about it?


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🅃🄴🄰🄼 🄸🄷🄾🄿


New to cultivation or the shroomery?  Observe the hitchhiker's guide

The Hitchhiker's Guide to the Shroomery



Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

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Re: Post Your New Tools Of The Trade. [Re: Mr Piggy] * 3
    #28681178 -

I believe that some folks are leaving plates in the SAB for long periods to dry out condensation. The minor disturbance you mentions is certainly not ideal, but is likely to be less detrimental than a day's worth of central air, breeze from open windows and shutting doors, general motion in the room, house flies, cats sticking curious heads inside, etc.

And as for the candle test, again I agree that there will be a small disturbance when adding and removing the covers. But use your same candle test while moving your hands and arms in and out of the box to sterilize your blade. Are you really creating less of a disturbance shoving your whole mitts in and out than just placing a flat surface over the holes?

Using covers during a session probably doesn't make sense, but that doesn't mean that it has no value at all for people using their SAB in other ways. If you have the luxury of a dedicated lab or locked off space with no air flow, you probably don't benefit from covers. But then you might just have the benefit of other things like a ffu or flow hood as well. If you are using your kitchen table as you lab, you may have different needs and limitations.


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Stoned Gummys | BRF Pucks | Primo Reusable Plates | Easy LI 4 Preserv & Propo
"Psychedelics are powerful substances. Nothing that powerful is completely safe... and nothing completely safe is that powerful!" - Abigail Calder at ALPS 2023
Don't Panic

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Re: Post Your New Tools Of The Trade. [Re: ReverendMyc] * 1
    #28681199 -

That's a great explanation thank you.  I forget not everyone has space set aside in a room for a setup.  Mine is in my bedroom, but I don't do anything that requires a sanitary space for long periods,  When I spore print it's under the SAB with holes open and I've never had a problem.

ReverendMyc said:
And as for the candle test, again I agree that there will be a small disturbance when adding and removing the covers. But use your same candle test while moving your hands and arms in and out of the box to sterilize your blade. Are you really creating less of a disturbance shoving your whole mitts in and out than just placing a flat surface over the holes?



This is the only part I disagree with.  Covers do create suction when removed and that can change the pressure in a SAB resulting in all of the air inside shifting.  Arms moving slowly through large open holes creates a little disturbance in their area, but does not move all of the air at once by changing the pressure in the box.


--------------------
🅃🄴🄰🄼 🄸🄷🄾🄿


New to cultivation or the shroomery?  Observe the hitchhiker's guide

The Hitchhiker's Guide to the Shroomery



Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

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Re: Post Your New Tools Of The Trade. [Re: Mr Piggy] * 2
    #28681210 -

I store a ton of shit in my SAB. Most of my active projects live in there. Arm hole covers are awesome. It stays open most of the time, but I close it up when I have it prepped for a session, making prints, house cleaning, keeping cats from exploring exciting new glassware to knock over etc. my covers are magnetic and slide on/off sideways pretty smoothly, no major disturbances.


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This hobby has taught me many things.
Most of those things are patience.

The Hitchhiker's Guide to the Shroomery
The Official Shroomery Cultivation Museum
CakeWars
III

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Re: Post Your New Tools Of The Trade. [Re: Scemilope] * 1
    #28681295 -

Another question for arm hole cover users:  Have you noticed/documented fewer contaminations with use of arm covers?  And is this due to the covers and not just honing technique?

I'm actually curious about this stuff :takingnotes:


--------------------
🅃🄴🄰🄼 🄸🄷🄾🄿


New to cultivation or the shroomery?  Observe the hitchhiker's guide

The Hitchhiker's Guide to the Shroomery



Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

Extras: Filter Print Post Top
Re: Post Your New Tools Of The Trade. [Re: Mr Piggy]
    #28681400 -

I am not arguing, just confused why placing a cover on the holes would create more of a pressure change than the piston action of inserting and removing arms. The SAB should not be air tight because it is just a tub resting on a table, in most cases. And even if it were air tight, putting a lid on a jar doesn't create a pressure differential on its own, so I don't see where the covers do.
PS: I would use an incense stick instead of a flame for more of a lasting visual of air movement.

But all of this is academic to me because I am an ffu snob now, so I can't even give a fair answer about improved or worsening contam rates with and without them any more. I added some made out of magnetic vent covers when I did use a SAB. But I have been very fortunate in having little trouble with contams. Probably more of a testament to low spore count in incredibly hot dry environment than to any actual skillz.


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Stoned Gummys | BRF Pucks | Primo Reusable Plates | Easy LI 4 Preserv & Propo
"Psychedelics are powerful substances. Nothing that powerful is completely safe... and nothing completely safe is that powerful!" - Abigail Calder at ALPS 2023
Don't Panic

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Re: Post Your New Tools Of The Trade. [Re: Mr Piggy] * 3
    #28681465 -



--------------------
This hobby has taught me many things.
Most of those things are patience.

The Hitchhiker's Guide to the Shroomery
The Official Shroomery Cultivation Museum
CakeWars
III

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Re: Post Your New Tools Of The Trade. [Re: Scemilope] * 1
    #28681503 -

Scemilope said:
I store a ton of shit in my SAB. Most of my active projects live in there. Arm hole covers are awesome. It stays open most of the time, but I close it up when I have it prepped for a session, making prints, house cleaning, keeping cats from exploring exciting new glassware to knock over etc. my covers are magnetic and slide on/off sideways pretty smoothly, no major disturbances.


I think this is a great reason to have arm-hole covers. I can absolutely imagine a cat sticking his arm into an SAB and making a mess.

I don't really store anything in my SAB. It gets tucked under my bed when it's not in use. But I would like to jump into the arm-hole cover debate. I have never used them or seen the need for them. I use my SAB in my living room/kitchen area, where people are walking around, there is air movement, and there are plenty of houseplants and other sources of spores/contamination.

When I pour agar in my SAB, I leave my plates in stacks of 10 to cool, usually for 12+ hours. I leave the arm-holes uncovered. I am sure that plenty of spores float their way into the SAB. The outer surfaces of my petri dishes are surely contaminated. When I come back to my cooled agar plates, I sanitize my hands and glove up, and then move the plates into Ziploc bags.

Before I use one of my agar dishes, I wipe down the exterior of the Ziploc with 70% isopropyl and a paper towel. Then pull the plates out, and wipe the exterior of each individual plate down with 70% isopropyl as well. In my opinion, you should be wiping EVERYTHING down with 70% isopropyl. So it shouldn't matter if the exterior surfaces get exposed to dirty air.


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Re: Post Your New Tools Of The Trade. [Re: joze] * 6
    #28681513 -




:wink:

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