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Shop: Myyco.com Isolated Cubensis Liquid Culture For Sale   Unfolding Nature Unfolding Nature: Being in the Implicate Order

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Re: Tek: Long-Term Storage of Cultures with Cryogenic Vials (Cryovials) [Re: Screwup] * 1
    #28043665 -

Dumb question but…obviously leave these cracked slightly while in the PC right? Didn’t see anything saying that.
No need to wrap in foil either?

Edited by Screwup (11/10/22 02:35 PM)

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Re: Tek: Long-Term Storage of Cultures with Cryogenic Vials (Cryovials) [Re: Screwup] * 1
    #28043672 -

Screwup said:
Dumb question but…obviously leave these cracked slightly while in the PC right? Didn’t see anything saying that.
No need to wrap in foil either?


Yes.. I put them in a creme brulee jar, cover with foil and pc

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Re: Tek: Long-Term Storage of Cultures with Cryogenic Vials (Cryovials) [Re: 0t0lerance] * 1
    #28043691 -

0t0lerance said:
Screwup said:
Dumb question but…obviously leave these cracked slightly while in the PC right? Didn’t see anything saying that.
No need to wrap in foil either?


Yes.. I put them in a creme brulee jar, cover with foil and pc


Cool thank you wanted to make sure lol. I threw em in a half pint and covered with foil

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Re: Tek: Long-Term Storage of Cultures with Cryogenic Vials (Cryovials) [Re: Screwup] * 1
    #28043772 -

Thank you Mycoplex! :takingnotes:

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Re: Tek: Long-Term Storage of Cultures with Cryogenic Vials (Cryovials) [Re: fungusul] * 1
    #28044598 -

This will come super in handy with the pink oysters I have since I’ve heard they’re finicky with refrigeration for long term storage. Thanks a bunch!

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Re: Tek: Long-Term Storage of Cultures with Cryogenic Vials (Cryovials) [Re: cerbes]
    #28055233 -

Glad to see that others are finding this tek helpful! :rockon:


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Cryovial Tek
Indoor Ovoid Community
ATL7 Cosmoplex Gallery
Fungus Gnat Control Tek
Precise Pasteurization Tek

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Re: Tek: Long-Term Storage of Cultures with Cryogenic Vials (Cryovials) [Re: Mycoplex] * 1
    #28055543 -

Mycoplex said:
Glad to see that others are finding this tek helpful! :rockon:


Yes, I've been prepping the vials of 1,8mm which contained a black silicon ring inside (transparant cap), however these 5ml ones with blue cap don't contain a silicone ring.. I'm a bit afraid they will leak on their sides, so keeping them upright at the moment. What is your experience with this? I was thinking about vacuum sealing them after adding a wedge.. but if they start leaking  on their sides, that is no good..

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Re: Tek: Long-Term Storage of Cultures with Cryogenic Vials (Cryovials) [Re: 0t0lerance] * 1
    #28055549 -

Cryovials can have a lip-seal, a silicon-seal or even both. So having one of a kind should totally suffice.


--------------------
I am not crazy, I prefer the term mentally hillarious.

Agar - The way to go!
Clean spawn checklist
Proper Surface Moisture
Recognizing and dealing with contamination
🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿

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Re: Tek: Long-Term Storage of Cultures with Cryogenic Vials (Cryovials) [Re: Shrimps] * 2
    #28101695 -

Oh boy. This is turning out way more complicated than I expected. Of course the sellers don't specify if the lids are made of the same material as the vial itself. I've seen one question about one product that asked this and the seller actually responded the lids are polystyrene 😬😬 Fuck me sideways. Don't wanna order 200 of these just for the lids to turn out Polystyrene.

Most of them are even so stupid to say the material is "plastic". I hope they all choke on dick one day.


--------------------
Shrooms are always bluer on the other side...

Work hard, play harder, trip the hardest.

Simple Cloning TEK by Adas
Semi-Sterile TEK (SS-TEK)
Growing Panaeolus bisporus - The Low-Tech approach
Exotic Mycelia Surviving Winter - Creating IceBreaker Variants!
My First Panaeolus Cyanescens Grow!
Psilocybe azurescens photo compilation 2020
Azurescens in June!

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Re: Tek: Long-Term Storage of Cultures with Cryogenic Vials (Cryovials) [Re: Adas] * 1
    #28101706 -

I think the only good ones are the pack of 500 that say they're PP5 and come with the holder too.


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Links For Beginners And Beyond:mspoil:

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Re: Tek: Long-Term Storage of Cultures with Cryogenic Vials (Cryovials) [Re: rumfor69] * 2
    #28101738 -

I bought mine at a Lab store. Might be a bit pricier than over Amazon but they're made in Germany and high quality. Don't really trust these chinese offers ^^


--------------------
I am not crazy, I prefer the term mentally hillarious.

Agar - The way to go!
Clean spawn checklist
Proper Surface Moisture
Recognizing and dealing with contamination
🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿

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Re: Tek: Long-Term Storage of Cultures with Cryogenic Vials (Cryovials) [Re: Adas] * 1
    #28101797 -

Adas said:
Oh boy. This is turning out way more complicated than I expected. Of course the sellers don't specify if the lids are made of the same material as the vial itself. I've seen one question about one product that asked this and the seller actually responded the lids are polystyrene 😬😬 Fuck me sideways. Don't wanna order 200 of these just for the lids to turn out Polystyrene.

Most of them are even so stupid to say the material is "plastic". I hope they all choke on dick one day.


Hahaha, I had the same pp5 filtering issue when shopping on aliexpress, after some digging purchased some, seem to work just fine. Checked the lids before-after for deformation or other defects.. couldn't detect it.. also the twisting action was not affected.. so crossing my finger it's 'good enough'

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Re: Tek: Long-Term Storage of Cultures with Cryogenic Vials (Cryovials) [Re: 0t0lerance] * 3
    #28101814 -

Yea mate, if it didn't straight up melt the first PC run, you're good.


--------------------
Shrooms are always bluer on the other side...

Work hard, play harder, trip the hardest.

Simple Cloning TEK by Adas
Semi-Sterile TEK (SS-TEK)
Growing Panaeolus bisporus - The Low-Tech approach
Exotic Mycelia Surviving Winter - Creating IceBreaker Variants!
My First Panaeolus Cyanescens Grow!
Psilocybe azurescens photo compilation 2020
Azurescens in June!

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Re: Tek: Long-Term Storage of Cultures with Cryogenic Vials (Cryovials) [Re: Adas] * 3
    #28117421 -

I also ran into a bit of the same ambiguity when buying the vials prior to making the writeup.  I think it has to do with a lot of the vendors being non-English speakers probably. In my experience these seem to be manufactured based on similar specs regardless of who the underlying vendor is, as though there is one schematic for this and most vendors just follow it.

I've bought the vials from two or three different vendors so far and I've found that as long as they have the logo/stamp "IMEC" they tend to be the right choice regardless of who the vendor is or how they describe it.  If you look at the sample pics in the writeup here or when searching for the vials, you can usually see this "IMEC" stamp on the vial.


--------------------
Cryovial Tek
Indoor Ovoid Community
ATL7 Cosmoplex Gallery
Fungus Gnat Control Tek
Precise Pasteurization Tek

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Re: Tek: Long-Term Storage of Cultures with Cryogenic Vials (Cryovials) [Re: Mycoplex] * 2
    #28117497 -

Interesting, potentially very useful info! Thanks Myco! :hug: I've already ordered some from German Amazon so we'll see how that goes. Seems they really are PP so.. fingers crossed.


--------------------
Shrooms are always bluer on the other side...

Work hard, play harder, trip the hardest.

Simple Cloning TEK by Adas
Semi-Sterile TEK (SS-TEK)
Growing Panaeolus bisporus - The Low-Tech approach
Exotic Mycelia Surviving Winter - Creating IceBreaker Variants!
My First Panaeolus Cyanescens Grow!
Psilocybe azurescens photo compilation 2020
Azurescens in June!

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Re: Tek: Long-Term Storage of Cultures with Cryogenic Vials (Cryovials) [Re: Adas] * 2
    #28180980 -

I've just placed first 5 cultures into the vials. Now starts the countdown :lol:

It was not hard but moving the tiny pieces into the vials is a bitch. Only 1 piece managed to fall off tho :rockon:


--------------------
Shrooms are always bluer on the other side...

Work hard, play harder, trip the hardest.

Simple Cloning TEK by Adas
Semi-Sterile TEK (SS-TEK)
Growing Panaeolus bisporus - The Low-Tech approach
Exotic Mycelia Surviving Winter - Creating IceBreaker Variants!
My First Panaeolus Cyanescens Grow!
Psilocybe azurescens photo compilation 2020
Azurescens in June!

Extras: Filter Print Post Top
Re: Tek: Long-Term Storage of Cultures with Cryogenic Vials (Cryovials) [Re: Adas] * 3
    #28181003 -

oh shit, i completely forgot about this thread but i’ve actually had some cultures in distilled storage for over a few months. i used quarter pint mason jars with rather large wedges for storage. put a culture in on 5/25/22 & put it  back on agar a few weeks ago & the myc grew out beautifully. i wanted to use quarter pints so that i could throw larger wedges in & also because i didn’t feel like waiting for vials to ship to me lol but they seem to work great. i just flip the lids they come with & run em stock


--------------------
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===================================
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posts i constantly refer back to
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Re: Tek: Long-Term Storage of Cultures with Cryogenic Vials (Cryovials) [Re: hazyhorse] * 1
    #28181017 -

just ordered my vials. has anyone here tried store fresh tissue instead of agar wedges?


--------------------
Confused? Well now you can!
HHG - cheapest way to start - how i roll

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Re: Tek: Long-Term Storage of Cultures with Cryogenic Vials (Cryovials) [Re: bakedbeings] * 1
    #28181019 -

I thought someone posted something about fresh fruits in a other thread where he also stored like colonized paper in vials as well.


--------------------
I am not crazy, I prefer the term mentally hillarious.

Agar - The way to go!
Clean spawn checklist
Proper Surface Moisture
Recognizing and dealing with contamination
🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿

Extras: Filter Print Post Top
Re: Tek: Long-Term Storage of Cultures with Cryogenic Vials (Cryovials) [Re: bakedbeings] * 2
    #28181021 -

I cant imagine there would be a significant difference.


--------------------
Shrooms are always bluer on the other side...

Work hard, play harder, trip the hardest.

Simple Cloning TEK by Adas
Semi-Sterile TEK (SS-TEK)
Growing Panaeolus bisporus - The Low-Tech approach
Exotic Mycelia Surviving Winter - Creating IceBreaker Variants!
My First Panaeolus Cyanescens Grow!
Psilocybe azurescens photo compilation 2020
Azurescens in June!

Extras: Filter Print Post Top
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Shop: Myyco.com Isolated Cubensis Liquid Culture For Sale   Unfolding Nature Unfolding Nature: Being in the Implicate Order

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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