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Sterilize scalple blades in PC
    #27719573 -

Curious if and how to sterilize scalple blades in a PC.

This might be a "cheap" approach but I'm thinking about not flaming  my blades between transfers. Using a "new" blade each time. 

And am wondering if I can PC for additional use.

My plan is after using, wrap in foil,  place multiple wrapped blades in a small jar, then place in PC when either doing "plates" (Holy Grails) or grain jars.

Any advice?

Regards-
Turbo

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Re: Sterilize scalple blades in PC [Re: Turbo0317] * 1
    #27719579 -

Wrap individually in foil and put into a small container, vent the lid, and PC for 20 min.
Why not flame?


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Crack's Tek's and Shit


Edited by Crackatoa (04/03/22 05:09 PM)

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Re: Sterilize scalple blades in PC [Re: Crackatoa]
    #27719590 -

I use a SAB so thought instead of moving hand(s) in and out of box use a new blade for each plate. Maybe this approach would/will stir the air same as removing for flaming?

How would one vent the lid? One holes or two using a "filter" or not?

Thanks-
Turbo

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Re: Sterilize scalple blades in PC [Re: Turbo0317]
    #27719597 -

A filter of sorts covering 1 small hole.

Sticker sfds work nice ime. Simple polyfil.. multiple mp tape layers also work:thumbup:


--------------------
:hypnotoad:
Making Liquid Culture :  Go insane with LC
Mushboy likes these teks : Making shroom tea
Dank Education

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Re: Sterilize scalple blades in PC [Re: mushboy]
    #27719621 -

Thanks Crack and Mush!

I have a super small Operation and I know v I'm being frugal but as I was making a few transfers today thought about my question posed.

Much appreciated-
Turbo

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Re: Sterilize scalple blades in PC [Re: Turbo0317]
    #27719630 -

I've always PC'd my knifes and find it extremely helpful. One great benefit is that you're able to use your scalpel much more freely, if you're for example moving a whole or a half agar culture for when you're making a LI for example then I find it much easier to lift and move huge pieces when you can use all parts of the knife and it doesn't matter if the culture touches the rest of the knife it's still all sterilized.



I used to wrap my scalpel in aluminum foil in the past (and maybe I'll get back to that at some point but not yet) but for now I just put them in a QT jar with filtered lid and PC for 15-30 minutes at 100C (212f) or 120C (250f). I just pour a very small amount of water in the QT jar just enough so that a mm or two of the blade tips are in the water at the bottom of the jar. Can look like this:




Just remember to not leave the knifes inside a wet jar for more than a day, this is for immediate use and even if you've just used 1 or 2 knifes out of 10 you should remove, clean and store all the knifes in a dry place until next SAB session :thumbup:


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Cakes inside Water Tub with optimal Surface Conditions

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Re: Sterilize scalple blades in PC [Re: Turbo0317]
    #27719659 -

I've PCed scalpel blades before.  They got a bit rusty but were still serviceable. I have some very small sterilization pouches I sterilized them in.  Also very handy for handles, LC syringes, etc.  They seem a little pricey up front, but they go a LONG way.  And oh so convenient.  Much more so than wrapping in foil (which was my previous go-to)



https://www.shroomery.org/forums/showflat.php/Number/27584568


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Agar and Liquid Culture Formulas


Equipment, Teks, Etc
FF Lid Tek

Edited by father_fungi (04/03/22 06:16 PM)

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Re: Sterilize scalple blades in PC [Re: father_fungi] * 1
    #27719701 -

I use these too. They're just like the pouches that presterilized syringes/needles come in but bigger.

But why not buy stuff presterilized? A new disposable blade is made to be affordable enough to use once and toss.

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Re: Sterilize scalple blades in PC [Re: Anonuser77] * 1
    #27719776 -

It's like trying to save a solo cup. I mean they're literally almost the same price...

PSA. Never put sharp stuff loosely in a jar, bag, or wrapped in foil. You could easily cut yourself. When presterilized blades come wrapped in thick paper. For 10 cents each.

Be professional.

Edited by Anonuser77 (04/03/22 07:29 PM)

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Re: Sterilize scalple blades in PC [Re: Anonuser77] * 3
    #27719788 -

I pick my scalpel up off the floor and wipe it with an alcohol wipe and flame the whole thing for 5 seconds. Same blade for eons.

PCing blades seems pretty much pointless and more likely to contam it trying to put it on, but you do what ya gotta do I guess.


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- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed

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Re: Sterilize scalple blades in PC [Re: sandman420] * 1
    #27720393 -

:whathesaid:

nothing wrong with a little iso and a blowtorch, tried and true ftw

been doing this three years and still using the blades that came with the exacto knife lol, still have most of them left

:snoopyes:

but otherwise if I'm the OP and I insist I'd have the autoclave pouches for sure :rockon:

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Re: Sterilize scalple blades in PC [Re: SingularFusion] * 5
    #27720432 -

I fuckinh despise this bs throwaway culture we are so happy to support.

Who cares how disposable scalpel blades, gloves are, reuse the things please.

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Re: Sterilize scalple blades in PC [Re: DERRAYLD]
    #27720498 -

:whathesaid:


I even reuse my gloves sometimes, mostly cause I'm lazy but I also feel good about using the gloves until they rip :bongload:


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Cakes inside Water Tub with optimal Surface Conditions

Edited by Mateja (04/04/22 09:05 AM)

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Re: Sterilize scalple blades in PC [Re: DERRAYLD]
    #27720505 -

DERRAYLD said:
I fuckinh despise this bs throwaway culture we are so happy to support.

Who cares how disposable scalpel blades, gloves are, reuse the things please.


This!
Hell, I reuse the ice cream cake packaging for cloning plants, over and over again.

Flaming is the best method. Less of a chance of contams if it is sterilized seconds before use.  Less handling, better success.


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Up to my elbows in shit!

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Re: Sterilize scalple blades in PC [Re: Mushit]
    #27720572 -

I go thru a few blades a sesh even with flaming them.

As much as "throw away culture" sucks... its how society is now a days. Lets face it, future generations are fucked and too many of the current ones dont give a shit so... might as well revel in watching it all burn.



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:mushroom2: LotKid's PE Tips        :shpongle:    LotKid's Chocolates :mushroom2:
:dancingbear: :dancingbear:

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Re: Sterilize scalple blades in PC [Re: LotKid]
    #27720597 -

LotKid said:
watching it all burn.



I used a burner once or twice in 2016 and haven't bothered with it since. Comfort wise, nothing beats having a qt jar full of sterile tools inside the SAB, just reach and grab whatever you need while your still holding the donor plate in your other hand (for example) lol


If OP has a flow hood then comfort isn't really an issue but if he/she's working inside a SAB then nothing beats having sterile tools ready inches from the workplace. My philosophy is when I'm working inside I don't wanna be taking my hands out all of the time, this minimizes the air turbulence and speeds up the work process which is good for minimizing the risk of contamination. Gl


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Cakes inside Water Tub with optimal Surface Conditions

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Re: Sterilize scalple blades in PC [Re: Mateja]
    #27720604 -

i argue it doesnt reduce the risk anymore than loading batches of quart jars. if you can do that then a scalpel in and out isnt going to make or break you. honestly if THATS a defining moment of sterile tek you need to redo your game.

my retarded ass has grown tons of shrooms by heating a scalpel over and over and sticking it inside the box with my hand. its red hot on the way in:shrug:


--------------------
:hypnotoad:
Making Liquid Culture :  Go insane with LC
Mushboy likes these teks : Making shroom tea
Dank Education

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Re: Sterilize scalple blades in PC [Re: mushboy] * 1
    #27720681 -

I pour thin agar plates and it's just not realistic to be able to move half a culture around safely/swiftly on the tip of a blade, it breaks up too easily. But when I use the scalpel as a shovel I can even move whole cultures around quickly. As said it's a comfort thing, when I know I can hover the scalpen inside/above sterile areas I don't need to sweat it and work is much faster (inside an SAB)


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Cakes inside Water Tub with optimal Surface Conditions

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Re: Sterilize scalple blades in PC [Re: Turbo0317]
    #27720700 -

There is no need to waste the energy pressure sterilizing scalpel blades. 

As mentioned above; as long as the blade is red hot when going from flame to inside of SAB, then it's fine. 

When the blade is red hot, the heat being released from the scalpel blade acts as a barrier.  The heat pushes air and contaminates away from the blade as it travels through the air.


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How I Get Stuff done. - My Reference Guide. - My Grows.

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Re: Sterilize scalple blades in PC [Re: Mateja]
    #27720732 -

Mateja said:
I pour thin agar plates and it's just not realistic to be able to move half a culture around safely/swiftly on the tip of a blade, it breaks up too easily. But when I use the scalpel as a shovel I can even move whole cultures around quickly. As said it's a comfort thing, when I know I can hover the scalpen inside/above sterile areas I don't need to sweat it and work is much faster (inside an SAB)


I do the same thing of course (use the handle as a shovel for large agar pieces) you can do that with a quick flame kiss of the handle. No need to make everything red hot.

Red hot is like 1200 degrees F everything dies on a non porous surface by the naked flame instantly but even a few seconds to get it to 300 is plenty.


--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed

Edited by sandman420 (04/04/22 01:43 PM)

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