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Offlineliquidism
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Registered: 09/15/04
Posts: 10
Last seen: 11 years, 3 months
Sterilization w/o PC or boiling
    #3590032 - 01/06/05 07:46 PM (11 years, 10 months ago)

I CANT pressure cook or use my stove to sterilize my jars(WBS) because it would attract unwanted attention. But after a little reading I found out that the bacterial endospores need to germinate before they can be killed. My question is how would I make the endospores germinate so I could then kill them by soaking them in water and H2O2? Or would the H2O2 even kill them at all?
Thanks for your time.


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OfflineIGnosticAbhorI
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Re: Sterilization w/o PC or boiling [Re: liquidism]
    #3590070 - 01/06/05 07:54 PM (11 years, 10 months ago)

Lol, My friend, you may want to read a bit more.

Endospores=Germs.

The endospores aren't just any germ though. In their dorment state they can withstand temps. that are so hot you'd shit your pants (about 600F) and for long periods of time as well.

Germination:
You make them germ. by soaking them water (or some liquid w/ nutes in it b/c they need something to germ. with, i.e Poo tea) to allow them to get ready to mate basically and thats when they let down their defenses but they can still withstand hotter temps than the boiling point for water and higher....So you have to PC them to get them hot enough, plus you have to do it for a long period of time (about 1h 2m), You can't just soak them in something and hope that they will die. It doesnt work like that, h2o2 won't kill the endospores Even if you soak, they could withstand, you could use bleach, But then you'd kill all the nutes as well...

Sorry about the bad news, but I'd hate to watch you waste a bunch of substrate and Time...Good luck tho

I apoligize if I forgot anything...

-Gnostic-


Edited by IGnosticAbhorI (01/06/05 07:56 PM)


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OfflineIGnosticAbhorI
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Re: Sterilization w/o PC or boiling [Re: IGnosticAbhorI]
    #3590094 - 01/06/05 07:58 PM (11 years, 10 months ago)

O, and you can get camping portable stove tops, you can just plug it in your room or somewhere and boil there....I think you can find them at wal-mart but i'm sure there's other places, you could try the net, first, thats what it's for :wink:

Gl


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InvisibleNOS4A2
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Registered: 07/22/04
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Re: Sterilization w/o PC or boiling [Re: liquidism]
    #3590119 - 01/06/05 08:02 PM (11 years, 10 months ago)

If you can't boil anything without feeling paranoid maybe you should wait untill you're a little older.


--------------------
Hail to the King, Baby
CulturingTech


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OfflineC4NN4B15_F4NN4B15
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Registered: 08/30/04
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Re: Sterilization w/o PC or boiling [Re: NOS4A2]
    #3590144 - 01/06/05 08:06 PM (11 years, 10 months ago)

i don't think thats why he dosen't want to boil or PC^^^^^^, BUT thats really the only options you have


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Offlineliquidism
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Registered: 09/15/04
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Re: Sterilization w/o PC or boiling [Re: IGnosticAbhorI]
    #3590150 - 01/06/05 08:08 PM (11 years, 10 months ago)

Ok...I understand that in their dormant state they are very resistant but non-dormant bacterica can be killed by H2O2(at least the ones in my wounds) right? Is there any other way? Theres gotta be someone here that knows of other methods. Help me think outside of the box.

NOS4A2...
I wasn't looking for smartass remarks...just some help. I'm old enough that if someone sees me boiling jars full of grain and called the police I would get put in jail. Some people have to share a kitchen with straight edge people.


Edited by liquidism (01/06/05 08:13 PM)


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InvisibleNOS4A2
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Registered: 07/22/04
Posts: 510
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Re: Sterilization w/o PC or boiling [Re: C4NN4B15_F4NN4B15]
    #3590152 - 01/06/05 08:08 PM (11 years, 10 months ago)

So how long would he have to boil?


--------------------
Hail to the King, Baby
CulturingTech


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Offlineticktock
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Re: Sterilization w/o PC or boiling [Re: NOS4A2]
    #3590616 - 01/06/05 09:38 PM (11 years, 10 months ago)

If you had some ham, you could make a ham sandwich....if you had some bread.

Sorry liquidism. The basic thing that you need first is a cooking surface. Heat, or anything else that kills germs, will kill your spores too. The heat goes away pretty soon. Bleach won't. You need to find a way to make a fire.


--------------------
Don't panic!


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Offlinebuckeye
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Registered: 01/19/04
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Re: Sterilization w/o PC or boiling [Re: ticktock]
    #3590807 - 01/06/05 10:12 PM (11 years, 10 months ago)

a pc is really needed in order to do grain.  You may want to visit the sponsors forum.  Spores101 is giving away free presterile jars. :thumbup:


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OfflineLupuz
It's 4:20Somewhere

Registered: 08/19/03
Posts: 38
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Last seen: 10 years, 10 months
Re: Sterilization w/o PC or boiling [Re: buckeye]
    #3590929 - 01/06/05 11:02 PM (11 years, 10 months ago)

Hell, there's your solution, buy some presterilized
jars. It costs a little more money than doing it
yourself but if you're worried about getting caught
(and I'm taking you at your word that you're old
enough to be here) then that's the way to go.

Don't take offense at what NOS4A2 said, we get a lot
of little kids in here saying 'If I buy spores with
a stolen credit card, and have them mailed to my parents
house, do you think they'll notice the Martha I'm using
to fruit them with in my closet?.'

Those are the ones that fuck things up for those of
us old enough to know better.

Lupuz


--------------------
Those who desire to give up Freedom in order to gain Security, will not have, nor do they deserve, either one - Thomas Jefferson


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InvisiblePrisoner#1M
Even Dumber ThanAdvertized!
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Registered: 01/22/03
Posts: 191,583
Loc: Pvt. Pubfag NutSuck
Trusted Cultivator
Re: Sterilization w/o PC or boiling [Re: liquidism]
    #3591002 - 01/06/05 11:24 PM (11 years, 10 months ago)

Quote:

liquidism said:
Some people have to share a kitchen with straight edge people.




maybe you should try showing these roommates a bit of respect, if something
were to happen and the police did show up, its not just yourself that goes to jail

on that note, I'm locking this thread.


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