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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Saving bacterial cultures by drying them out [Re: fahtster]
    #27588791 -

I'm an idiot..I thought this entire TEK was saying you just dried the grains to kill the bacteria then you just used the dry grains to inoculate new, fresh grains :facepalm: reading has never been one of my strong suits but damn I didn't know it was this bad lol


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I :heart: 5318008


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Re: Saving bacterial cultures by drying them out [Re: bigfootscreepyuncl]
    #27588794 -

I mean, that’s pretty much what it is, there’s just water involved.  I inject water into the dried grains, shake the grains around to knock the myc off them and then extract the water and that’s used to inoculate new grains.  Sorry, I should’ve explained that

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Re: Saving bacterial cultures by drying them out [Re: fahtster]
    #27588800 -

No, I doubt anyone else had issues figuring that out. I really do read at like a 6th grade level lol. Plus the whole GLC thing shoulda been a dead giveaway. If I can't wrap my noob head around it, it's probably out of my league anyway :smile: you da man, Faht I love your lowTEKs


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I :heart: 5318008


NOT a virgin!

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Re: Saving bacterial cultures by drying them out [Re: fahtster]
    #27589744 -

fahtster said:
I dry my cultures out for storage purposes.. I use these lids.  They allow me to adjust the GE from 98% covered (during colonization) to 40% exposed (for drying out).  I stick them in front of a fan to speed the process up.  But I’ve found that drying the cultures out has a great side benefit of cleaning them up from bacteria (if there’s mold, the cultures facked).


Faht



I was wondering or wanting your thoughts on using a desiccant to speed up the drying process on your grain cultures.

I now dry all my prints in Home Dry from Costco an now I have cured my bacteria problem with my prints.


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Re: Saving bacterial cultures by drying them out [Re: micelio]
    #27589894 -

oh snap...should i be putting my prints in the dehydrator???


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Confused? Well now you can!
HHG - cheapest way to start - how i roll

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Re: Saving bacterial cultures by drying them out [Re: micelio]
    #27589916 -

Bakedbeings said:
oh snap...should i be putting my prints in the dehydrator???


No.. he’s talking about just using desiccant.. you don’t want air blowing all over your print or high heat killing the spores

micelio said:
fahtster said:
I dry my cultures out for storage purposes.. I use these lids.  They allow me to adjust the GE from 98% covered (during colonization) to 40% exposed (for drying out).  I stick them in front of a fan to speed the process up.  But I’ve found that drying the cultures out has a great side benefit of cleaning them up from bacteria (if there’s mold, the cultures facked).


Faht



I was wondering or wanting your thoughts on using a desiccant to speed up the drying process on your grain cultures.

I now dry all my prints in Home Dry from Costco an now I have cured my bacteria problem with my prints.


I think it’d work for sure.. just gotta do it properly.. it’d be like drying out fruits.. gotta make sure the desiccant is charged and you don’t end up with sealed up grain that’s not drying out but I don’t see why it wouldn’t work

Faht

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Re: Saving bacterial cultures by drying them out [Re: fahtster]
    #27595901 -

Alright.. visible regrowth @ 6 days.. things lookin good.  No excessive sweating (pretty big temp swings during the night, even with a small room heater).  Any liquid in the jar around individual kernels is crystal clear.. filter smells like cooked grain. 


Grains are well hydrated, if there’s any bacteria, it shouldn’t have a problem getting a foothold

Faht

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Re: Saving bacterial cultures by drying them out [Re: fahtster] * 1
    #27598631 -

I dried this colonize grain in 2 days. The grain is red milo (sorghum)

I placed the grain in a small container and into the jug of Dry Home from Costco..

The grain after 48 hours is rock hard.






The picture below Is dried colonize grain.



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Re: Saving bacterial cultures by drying them out [Re: micelio]
    #27598639 -

Cool!  Is it still clean?  Been done all in front of the hood?

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Re: Saving bacterial cultures by drying them out [Re: fahtster]
    #27598685 -

I wasn't concentrating on being sterile. I just wanted for now to cut down on drying time, so it's not a long time project.

I think I would use a sterilize jelly jar and some kind of filter that would allow moisture to pass through.

Maybe Someone would have some ideas, but I don't want to hog your thread..:lol:


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Re: Saving bacterial cultures by drying them out [Re: micelio]
    #27598753 -

Nah.. you’re all good.  Your post is welcome for sure!  I personally can really use that since I do use large filters for drying out so thnx for that :thumbup:

Faht

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Re: Saving bacterial cultures by drying them out [Re: fahtster]
    #27605868 -

A little update on these qts.. things are good.  They’re moving a little slower than usual but the culture was started on rye and these qts are wheat so I attribute some slowness to that for sure.  They look good..


I went ahead a shook them again.. I figure if there is bacteria, that should make em stall out but I’m not expecting them to.. I’m actually really excited to see what the tub from these does.. I’ve only seen the culture perform bacterial.  Not counting my chickens yet so let’s see what happens


Faht

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Re: Saving bacterial cultures by drying them out [Re: fahtster]
    #27606828 -

Great idea! :heart: to read your posts.

:takingnotes:

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Re: Saving bacterial cultures by drying them out [Re: fahtster]
    #27606948 -

Hey Faht..

I live on a small wheat grass farm.

There's 800 lbs. of fresh harvested wheat 15 foot from me right now as I type.

Last week the farmer ask me why I'm not using his wheat.

My reply to him, There's too much Bacteria..


I just bought 50 kilos of red milo/ sorghum.. last week and paid 500 pesos (25.00 dollars)

I have had real good luck with milo in Spanish It's called Sorgos.

The trick that I have found Is to only boil no longer in rapid boiling water 5 minutes. Then dry It on a screen rack for 30 to 45 min.

No pre-soak just rinse It three time to get out the dirt.


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Re: Saving bacterial cultures by drying them out [Re: micelio]
    #27607131 -

Nice.. that’s a good tip :thumbup:  I actually just switched to wheat.. I was using primarily rye.  I wasn’t having troubles with bacteria due to improper pc’ing.. my problems were coming from using SHIPs and occasionally from cloning.  Since switching to wheat and using the sream “pen”, I haven’t had any issues.  Actually, the wheat I’ve got is probably the cleanest (as in little dirt) grain I’ve tried so far but I’m sure there’s dirty wheat out there.

But that sounds like an easy af prep you’re doing and cheap too.

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Re: Saving bacterial cultures by drying them out [Re: fahtster]
    #27607266 -

I don't trust SHIPs. for one good reason is I have to use a 16 gauge needle If I want to suck up my lc.

Well after a few pokes through the SHIP I don't think they're sealing to well to be honest, especially If you make a  jab In the same spot over and over.


This is my non sealing port that works so well that there's no need to change this method or modify It.

I trust this method and been using it for a few years now.





When I pull my grain jars (33) from my AA941 They all go in front of the flow for cool down.

After they are cool I place my dust cover (Sandwich bag) over each jar and a rubber band around the bottom so no air or contaminates can draft up the side of the jar.

I have a 7/64 drill hole in the lid. The hole is big enough to accept my 5" 12 gauge blunt tip all stainless steel Luer lock on a 60ml. syringe. I took the needle to the grinder for a nice sharp point that goes through masking tape that covers the hole. when done I slap another piece of tape over the hole. and replace the dust cover.

I always peel the tape off and replace It with new piece after I'm done with the jar and after the jar goes through the washing..

I never touch the part of the tape that covers the hole.


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Re: Saving bacterial cultures by drying them out [Re: micelio]
    #27608547 -

Yeah.. that’s the gauge needle I use and why I have to use GLC.. I don’t have a hood so SHIPs are what I’m using.. I won’t open a jar or expose the inside to room air.

I also don’t like growing through the same port hole so I make my own large ones.  Glad you’re found a system that works for ya :thumbup:

The recovery on the f5 clone qts after the second shake is good and they should be done in a day or two

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Re: Saving bacterial cultures by drying them out [Re: fahtster]
    #27611799 -

Aight.. spawned the p+ f5 qts today..


They probably do still have a touch of bacteria.. they smelled great before and after I broke them up but there are some uncolonized grains here and there and a decent amount of condensation but idk if that’s because of the temp swings in the room and/or because I hydrate the grains more than most… and most of the uncolonized grains are at the bottom where the water collected.  Idk… but what I do know is that had I not dried them out for that amount of time, given the state of the culture when I started drying them out, they wouldn’t even have made it past the first shake.  I also live in a part of the country where it rains constantly during the winter so I don’t think the rh gets low enough to successfully rid the culture completely of bacteria with a fan only.. it would explain why after being dried out over the summer they come back spotless.. there’s like 2 months of little to no rain over the summer and from what I’ve read, bacteria needs less than 10% rh to be killed on a dry surface

I really like micellos damprid idea and will be definitely using it during the winter months when needed. 

This isn’t over, I still have the second glc jar that’s been chillin this whole time and I’m going to go a decent amount of time more before I use it and probably gonna do the damprid trick with it a few days before I use it and see how the jars from that compares to the above jars… I’ll be updating the thread with how the tub does, good or bad.. a lot can still happen but I’ve spawned waaaaay worse looking jars than those^^^ and gone multiple flushes so I’m staying optimistic :popcorn:

Faht

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Re: Saving bacterial cultures by drying them out [Re: fahtster]
    #27620362 -

Tub is looking good so far.. I made the tub with a 1/2” compressed top layer


Faht

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Re: Saving bacterial cultures by drying them out [Re: fahtster]
    #27625100 -

How's that tub comin' along faht? :popcorn:



One thing I especially like about this thread is that you acknowledge contaminations and are providing a way to deal with it instead of just telling everyone to toss it and give up hope/start fresh. I think a lot of us noobs, beginners and novices get a few bacterial jars or a green tub and think we're failures because we never see anyone show their hardships, let alone a veteran TC


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I :heart: 5318008


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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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