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OfflineHillBilly777
Heterotroph

Registered: 04/23/03
Posts: 83
Last seen: 11 years, 6 months
Help! How to save this strain?
    #1965358 - 09/30/03 10:12 AM (13 years, 2 months ago)

Hey, my friend's friend said he's got two cambo cakes going, one of which is fighting a trich bloom. He says he needs to save a culture or get some prints from these cakes or he'll have to re-order this strain. What do y'all think would be his best approach?


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Invisiblear393
old timer
Male
Registered: 01/27/00
Posts: 696
Loc: NYC, NY, US
Re: Help! How to save this strain? [Re: HillBilly777]
    #1965385 - 09/30/03 10:23 AM (13 years, 2 months ago)

agar plates, grab a tissue sample from one of the fruits and grow it out on agar and then do a couple of transfers to make sure you have clean myco. then you can drop some agar in some karo/honey water and then you can use that to innoculate your next batch of jars.
-ar393


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OfflineHillBilly777
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Registered: 04/23/03
Posts: 83
Last seen: 11 years, 6 months
Re: Help! How to save this strain? [Re: ar393]
    #1965439 - 09/30/03 10:44 AM (13 years, 2 months ago)

Thanks man, but he doesn't have any agar (wishes he did...gettin' some soon). Could he just get medieval on a chunk of the cake with some H2O2 then drop that in a karo jar?


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Invisiblear393
old timer
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Registered: 01/27/00
Posts: 696
Loc: NYC, NY, US
Re: Help! How to save this strain? [Re: HillBilly777]
    #1965473 - 09/30/03 10:57 AM (13 years, 2 months ago)

umm, you could try it...chances of success are very very low.

If my fish was growing cambos I would send you print but alas the fish is not. Try ralph (free spore ring) for cheap and viable spores.
-ar393


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Invisiblear393
old timer
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Registered: 01/27/00
Posts: 696
Loc: NYC, NY, US
Re: Help! How to save this strain? [Re: ar393]
    #1965481 - 09/30/03 11:01 AM (13 years, 2 months ago)

also the best way that i would think that you could try get part of that cake from the inside and using that. but before you get cutting into the cake i would spray that fucker (360) with alcohol and then break it open with your (gloved) hands so that the tool that you would use to cut the cake doesnt carry contams from the outside into the cake as you cut it. then with a clean knife i would grab a small chunk of colonized middle and then plop that sucker into an h2o2 bath and then using a clean (flamed, etc) pair of tweezers to grab a small bit of the h2o2ed chunk and then use it for the karo jar.
-ar393


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OfflineHillBilly777
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Registered: 04/23/03
Posts: 83
Last seen: 11 years, 6 months
Re: Help! How to save this strain? [Re: ar393]
    #1965488 - 09/30/03 11:10 AM (13 years, 2 months ago)

Thanks a lot for the help. SWIM can't wait to get some agar plates, tired of fucking around with lesser methods.


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Invisiblepsilomonkey
Twisted brainwrong of a oneoff man mental

Registered: 08/08/03
Posts: 812
Loc: Airstrip One
Re: Help! How to save this strain? [Re: HillBilly777]
    #1965556 - 09/30/03 11:58 AM (13 years, 2 months ago)

I would cut a section from the cake as far from the infection as possible, cut into small sections (about thumb tip size chucks) and quickly dip in h2o2 (straight 3% solution). Use these sections to innoculate a bunch of jars, with luck one or 2 will not be contam'ed.

But Agar is the best way to go if you can.


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Amazon Shop for: ½ Pint Jars, Agar

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