Home | Community | Message Board

Cannabis Seeds - Original Sensible Seeds
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Unfolding Nature Unfolding Nature: Being in the Implicate Order   North Spore Injection Grain Bag   MagicBag.co All-In-One Bags That Don't Suck   Mushroom-Hut Liquid Cultures   Myyco.com Isolated Cubensis Liquid Culture For Sale   OlympusMyco.com Olympus Myco Polypropylene Grow Bags

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflineCosmicTourist
Stranger
Registered: 02/25/03
Posts: 24
Last seen: 19 years, 3 months
Saving a contaminated jar?
    #1456983 - 04/14/03 12:04 PM (21 years, 2 months ago)

My dog has a jar of pes Amazonian that developed a yellow spot right next to a large mycelium patch.  he wants to save this jar because it is the only one in the batch that has shown growth so far and it might be the only one.  He injected an 6cc:1cc water and h202 mix onto the yellow patch. 
---is it possible for the mycelium to simply overgrow the competitor and destroy it?
---Can my dog open the jar and take some mycelium from the other side of the jar, mix this up in a jar of water, wait for it to settle, then make a mycelium syringe which should indefinetly colonize multiple jars quickly?

My camera is out of bateries, but i may have a picture later today.  thanks in advance, can't wait to hear the replies :smile:

Extras: Filter Print Post Top
OfflineCosmicTourist
Stranger
Registered: 02/25/03
Posts: 24
Last seen: 19 years, 3 months
Re: Saving a contaminated jar? [Re: CosmicTourist]
    #1457107 - 04/14/03 01:10 PM (21 years, 2 months ago)

anybody? Will the mycelium prevail????

Extras: Filter Print Post Top
Invisiblez@z.com
Libertarian
Registered: 10/13/02
Posts: 2,876
Loc: ATL
Re: Saving a contaminated jar? [Re: CosmicTourist]
    #1457157 - 04/14/03 01:33 PM (21 years, 2 months ago)

Quote:


---is it possible for the mycelium to simply overgrow the competitor and destroy it?




Yes, but it doesn't usually work out that way.]
Quote:


---Can my dog open the jar and take some mycelium from the other side of the jar, mix this up in a jar of water, wait for it to settle, then make a mycelium syringe which should indefinetly colonize multiple jars quickly?




You can, but chances are you will get even more contamination doing that.

The best thing you can do is just keep an eye on that yellow spot and hope for the best. Are you sure it isn't just mycelium piss?


--------------------
"Of all tyrannies, a tyranny exercised for the good of its victims may be the most oppressive. It may be better to live under robber barons than under omnipotent moral busybodies. The robber baron's cruelty may sometimes sleep, his cupidity may at some point be satiated; but those who torment us for our own good will torment us without end, for they do so with the approval of their own conscience." - C.S. Lewis

"I would rather be exposed to the inconveniencies attending too much liberty than to those attending too small a degree of it." - Thomas Jefferson

Edited by z@z.com (04/14/03 01:34 PM)

Extras: Filter Print Post Top
Offlinemesq
Psychonaut
 User Gallery

Registered: 02/24/02
Posts: 284
Last seen: 8 years, 9 months
Re: Saving a contaminated jar? [Re: z@z.com]
    #1457279 - 04/14/03 02:26 PM (21 years, 2 months ago)

What about transferring mycelial growth to peroxidated agar... wont save the jar but it will save the culture..

Extras: Filter Print Post Top
OfflineOddish
Bulbasaur!
Male

Registered: 04/12/03
Posts: 327
Last seen: 10 years, 8 months
Re: Saving a contaminated jar? [Re: z@z.com]
    #1457293 - 04/14/03 02:29 PM (21 years, 2 months ago)

What exactly is mycelium piss?

Extras: Filter Print Post Top
OfflineCosmicTourist
Stranger
Registered: 02/25/03
Posts: 24
Last seen: 19 years, 3 months
Re: Saving a contaminated jar? [Re: Oddish]
    #1457319 - 04/14/03 02:37 PM (21 years, 2 months ago)

mycelium piss is yellow liquidy excreation, but this jar is only a week old, and the spot is round and probably growing...

why wouldn't mycelium from the other side of the jar be good for a mycelium syringe? what if i transfered the mycel. to a honeywater jar with some peroxide in there. Shouldn't the peroxide take care of the yellow mold if there even is any on that side of the jar?

Extras: Filter Print Post Top
OfflineCosmicTourist
Stranger
Registered: 02/25/03
Posts: 24
Last seen: 19 years, 3 months
Re: Saving a contaminated jar? [Re: CosmicTourist]
    #1457322 - 04/14/03 02:38 PM (21 years, 2 months ago)

was the injected peroxide in the jar a good idea? has anyone done that before?

Extras: Filter Print Post Top
OfflineOddish
Bulbasaur!
Male

Registered: 04/12/03
Posts: 327
Last seen: 10 years, 8 months
Re: Saving a contaminated jar? [Re: CosmicTourist]
    #1457472 - 04/14/03 03:15 PM (21 years, 2 months ago)

So this yellow liquid excretion isn't harmful? It's just a byproduct of the mycelium?

Extras: Filter Print Post Top
OfflineMAIA
World-BridgerKartikeya (DftS)
Male User Gallery

Registered: 04/27/01
Posts: 7,397
Loc: Erra - 20 Tauri - M45 Sta...
Last seen: 3 days, 14 hours
Re: Saving a contaminated jar? [Re: Oddish]
    #1457578 - 04/14/03 03:57 PM (21 years, 2 months ago)

Quote:

So this yellow liquid excretion isn't harmful?




No, it isn't harmful by itself but its acumulation leads to a proper environment for contamination, i.e. Anaerobic bacteria.

Quote:

It's just a byproduct of the mycelium?




Yes, it is.

MAIA



--------------------
Spiritual being, living a human experience ... The Shroomery Mandala



Use, do not abuse; neither abstinence nor excess ever renders man happy.
Voltaire

Extras: Filter Print Post Top
OfflineMAIA
World-BridgerKartikeya (DftS)
Male User Gallery

Registered: 04/27/01
Posts: 7,397
Loc: Erra - 20 Tauri - M45 Sta...
Last seen: 3 days, 14 hours
Re: Saving a contaminated jar? [Re: CosmicTourist]
    #1457611 - 04/14/03 04:07 PM (21 years, 2 months ago)

Quote:

is it possible for the mycelium to simply overgrow the competitor and destroy it?




As others said, yes it's possible.

Quote:

Can my dog open the jar and take some mycelium from the other side of the jar, mix this up in a jar of water, wait for it to settle, then make a mycelium syringe which should indefinetly colonize multiple jars quickly?




Well, it actually depends on the nature of the contamination and your skill handling this task but it's possible. Don't take the cake out of the jar, just cut a small healthy portion of the mycelium carefully and dunk it in a h2o2:h2o solution. Then the fastest and safest way would be using the mycelium directly has spawn in new prepared jars, you do this by using a glovebox, cutting the mycelium inside the glovebox and doing the transfer with open lids.
Read our faq on the issues glovebox and mycelium transfers.

MAIA


--------------------
Spiritual being, living a human experience ... The Shroomery Mandala



Use, do not abuse; neither abstinence nor excess ever renders man happy.
Voltaire

Edited by MAIA (04/14/03 04:13 PM)

Extras: Filter Print Post Top
OfflineMAIA
World-BridgerKartikeya (DftS)
Male User Gallery

Registered: 04/27/01
Posts: 7,397
Loc: Erra - 20 Tauri - M45 Sta...
Last seen: 3 days, 14 hours
Re: Saving a contaminated jar? [Re: CosmicTourist]
    #1457632 - 04/14/03 04:12 PM (21 years, 2 months ago)

Quote:

was the injected peroxide in the jar a good idea? has anyone done that before?




Not a good idea.
You're messing with the water content which is no good. Also the contaminant gets exposed to the perioxide just in a short period of time, just enough to cause superficial damage, nothing more.

MAIA


--------------------
Spiritual being, living a human experience ... The Shroomery Mandala



Use, do not abuse; neither abstinence nor excess ever renders man happy.
Voltaire

Extras: Filter Print Post Top
OfflineCosmicTourist
Stranger
Registered: 02/25/03
Posts: 24
Last seen: 19 years, 3 months
Re: Saving a contaminated jar? [Re: MAIA]
    #1458884 - 04/14/03 10:40 PM (21 years, 2 months ago)

Thanks for the info dude. I think my dog wants to try dextrose water inoculation in a glove box perhaps. Do you know any good ratios of dextrose to water? hes gona put 1.5 tablespoons of "Equal" into a 1/2 pint jar of water then boil it for a half hour. he cant get any honey right now, but just plain dextrose should be fine right?

Extras: Filter Print Post Top
OfflineMAIA
World-BridgerKartikeya (DftS)
Male User Gallery

Registered: 04/27/01
Posts: 7,397
Loc: Erra - 20 Tauri - M45 Sta...
Last seen: 3 days, 14 hours
Re: Saving a contaminated jar? [Re: CosmicTourist]
    #1459739 - 04/15/03 08:22 AM (21 years, 2 months ago)

Sorry but i've never worked with dextrose before.
Why don't you just make a direct transfer ? Then after growing, make prints right away. I don't see why you want to have all that work, plus, dextrose as honey water is very prone to contams. Your call.

MAIA


--------------------
Spiritual being, living a human experience ... The Shroomery Mandala



Use, do not abuse; neither abstinence nor excess ever renders man happy.
Voltaire

Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Unfolding Nature Unfolding Nature: Being in the Implicate Order   North Spore Injection Grain Bag   MagicBag.co All-In-One Bags That Don't Suck   Mushroom-Hut Liquid Cultures   Myyco.com Isolated Cubensis Liquid Culture For Sale   OlympusMyco.com Olympus Myco Polypropylene Grow Bags


Similar ThreadsPosterViewsRepliesLast post
* saving infected jars??? guardian 2,049 5 04/02/02 07:31 AM
by littleone
* contaminated jar rejuvination fig 2,746 4 03/02/02 06:34 PM
by canid
* disposal of contaminated jars? Shiznitz 3,588 8 08/06/01 09:15 AM
by dopemaster
* Contaminated Jar Recovery. motatraehrehtom 1,859 4 12/16/02 12:48 PM
by motatraehrehtom
* is this a good idea for slightly contaminated jars Funkstah 1,525 3 06/10/02 07:13 AM
by MAIA
* what do you do with contaminated jars? ElectricGnome 2,248 4 07/18/02 01:57 AM
by Hippie3
* Help! Newby who sniffed possibly contaminated jar!!!!! SlapnutRob 1,233 1 03/31/03 01:47 PM
by MAIA
* Inhalation of contamination MushyMay 2,520 7 03/01/02 09:00 PM
by LilDon697

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
3,533 topic views. 8 members, 92 guests and 72 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.022 seconds spending 0.006 seconds on 12 queries.