|
 Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
 CosmicTourist
Stranger
Registered: 02/25/03
Posts: 24
Last seen: 19 years, 1 month
|
 Saving a contaminated jar? 
#1456983 - 04/14/03 12:04 PM (21 years, 8 days ago) |
|
|
My dog has a jar of pes Amazonian that developed a yellow spot right next to a large mycelium patch. he wants to save this jar because it is the only one in the batch that has shown growth so far and it might be the only one. He injected an 6cc:1cc water and h202 mix onto the yellow patch.
---is it possible for the mycelium to simply overgrow the competitor and destroy it?
---Can my dog open the jar and take some mycelium from the other side of the jar, mix this up in a jar of water, wait for it to settle, then make a mycelium syringe which should indefinetly colonize multiple jars quickly?
My camera is out of bateries, but i may have a picture later today. thanks in advance, can't wait to hear the replies 
|
CosmicTourist
Stranger
Registered: 02/25/03
Posts: 24
Last seen: 19 years, 1 month
|
|
anybody? Will the mycelium prevail????
|
 z@z.com
Libertarian
Registered: 10/13/02
Posts: 2,876
Loc: ATL
|
|
Quote:
---is it possible for the mycelium to simply overgrow the competitor and destroy it?
Yes, but it doesn't usually work out that way.]
Quote:
---Can my dog open the jar and take some mycelium from the other side of the jar, mix this up in a jar of water, wait for it to settle, then make a mycelium syringe which should indefinetly colonize multiple jars quickly?
You can, but chances are you will get even more contamination doing that.
The best thing you can do is just keep an eye on that yellow spot and hope for the best. Are you sure it isn't just mycelium piss?
--------------------
"Of all tyrannies, a tyranny exercised for the good of its victims may be the most oppressive. It may be better to live under robber barons than under omnipotent moral busybodies. The robber baron's cruelty may sometimes sleep, his cupidity may at some point be satiated; but those who torment us for our own good will torment us without end, for they do so with the approval of their own conscience." - C.S. Lewis
"I would rather be exposed to the inconveniencies attending too much liberty than to those attending too small a degree of it." - Thomas Jefferson
Edited by z@z.com (04/14/03 01:34 PM)
|
mesq
Psychonaut
Registered: 02/24/02
Posts: 284
Last seen: 8 years, 7 months
|
 Re: Saving a contaminated jar? [Re: z@z.com]
#1457279 - 04/14/03 02:26 PM (21 years, 7 days ago) |
|
|
What about transferring mycelial growth to peroxidated agar... wont save the jar but it will save the culture..
|
Oddish
Bulbasaur!
Registered: 04/12/03
Posts: 327
Last seen: 10 years, 6 months
|
 Re: Saving a contaminated jar? [Re: z@z.com]
#1457293 - 04/14/03 02:29 PM (21 years, 7 days ago) |
|
|
What exactly is mycelium piss?
|
CosmicTourist
Stranger
Registered: 02/25/03
Posts: 24
Last seen: 19 years, 1 month
|
Re: Saving a contaminated jar? [Re: Oddish]
#1457319 - 04/14/03 02:37 PM (21 years, 7 days ago) |
|
|
mycelium piss is yellow liquidy excreation, but this jar is only a week old, and the spot is round and probably growing...
why wouldn't mycelium from the other side of the jar be good for a mycelium syringe? what if i transfered the mycel. to a honeywater jar with some peroxide in there. Shouldn't the peroxide take care of the yellow mold if there even is any on that side of the jar?
|
CosmicTourist
Stranger
Registered: 02/25/03
Posts: 24
Last seen: 19 years, 1 month
|
|
was the injected peroxide in the jar a good idea? has anyone done that before?
|
Oddish
Bulbasaur!
Registered: 04/12/03
Posts: 327
Last seen: 10 years, 6 months
|
|
So this yellow liquid excretion isn't harmful? It's just a byproduct of the mycelium?
|
MAIA
World-BridgerKartikeya (DftS)
Registered: 04/27/01
Posts: 7,396
Loc: Erra - 20 Tauri - M45 Sta...
Last seen: 3 months, 4 days
|
Re: Saving a contaminated jar? [Re: Oddish]
#1457578 - 04/14/03 03:57 PM (21 years, 7 days ago) |
|
|
Quote:
So this yellow liquid excretion isn't harmful?
No, it isn't harmful by itself but its acumulation leads to a proper environment for contamination, i.e. Anaerobic bacteria.
Quote:
It's just a byproduct of the mycelium?
Yes, it is.
MAIA
--------------------
Spiritual being, living a human experience ... The Shroomery Mandala
 
Use, do not abuse; neither abstinence nor excess ever renders man happy.
Voltaire
|
MAIA
World-BridgerKartikeya (DftS)
Registered: 04/27/01
Posts: 7,396
Loc: Erra - 20 Tauri - M45 Sta...
Last seen: 3 months, 4 days
|
|
Quote:
is it possible for the mycelium to simply overgrow the competitor and destroy it?
As others said, yes it's possible.
Quote:
Can my dog open the jar and take some mycelium from the other side of the jar, mix this up in a jar of water, wait for it to settle, then make a mycelium syringe which should indefinetly colonize multiple jars quickly?
Well, it actually depends on the nature of the contamination and your skill handling this task but it's possible. Don't take the cake out of the jar, just cut a small healthy portion of the mycelium carefully and dunk it in a h2o2:h2o solution. Then the fastest and safest way would be using the mycelium directly has spawn in new prepared jars, you do this by using a glovebox, cutting the mycelium inside the glovebox and doing the transfer with open lids.
Read our faq on the issues glovebox and mycelium transfers.
MAIA
--------------------
Spiritual being, living a human experience ... The Shroomery Mandala
Use, do not abuse; neither abstinence nor excess ever renders man happy.
Voltaire
Edited by MAIA (04/14/03 04:13 PM)
|
MAIA
World-BridgerKartikeya (DftS)
Registered: 04/27/01
Posts: 7,396
Loc: Erra - 20 Tauri - M45 Sta...
Last seen: 3 months, 4 days
|
|
Quote:
was the injected peroxide in the jar a good idea? has anyone done that before?
Not a good idea.
You're messing with the water content which is no good. Also the contaminant gets exposed to the perioxide just in a short period of time, just enough to cause superficial damage, nothing more.
MAIA
--------------------
Spiritual being, living a human experience ... The Shroomery Mandala
Use, do not abuse; neither abstinence nor excess ever renders man happy.
Voltaire
|
CosmicTourist
Stranger
Registered: 02/25/03
Posts: 24
Last seen: 19 years, 1 month
|
Re: Saving a contaminated jar? [Re: MAIA]
#1458884 - 04/14/03 10:40 PM (21 years, 7 days ago) |
|
|
Thanks for the info dude. I think my dog wants to try dextrose water inoculation in a glove box perhaps. Do you know any good ratios of dextrose to water? hes gona put 1.5 tablespoons of "Equal" into a 1/2 pint jar of water then boil it for a half hour. he cant get any honey right now, but just plain dextrose should be fine right?
|
MAIA
World-BridgerKartikeya (DftS)
Registered: 04/27/01
Posts: 7,396
Loc: Erra - 20 Tauri - M45 Sta...
Last seen: 3 months, 4 days
|
|
Sorry but i've never worked with dextrose before.
Why don't you just make a direct transfer ? Then after growing, make prints right away. I don't see why you want to have all that work, plus, dextrose as honey water is very prone to contams. Your call.
MAIA
--------------------
Spiritual being, living a human experience ... The Shroomery Mandala
Use, do not abuse; neither abstinence nor excess ever renders man happy.
Voltaire
|
|
Threaded
Previous
Index
Next
All-In-One Bags That Don't Suck 
Isolated Cubensis Liquid Culture For Sale 
Liquid Cultures 
Sterilized Grain Bag 
Injection Grain Bag 
Unfolding Nature: Being in the Implicate Order
Edit 
Reply 
guardian
fig
motatraehrehtom
ElectricGnome
SlapnutRob
MushyMay