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Stipe-n Cap
Full retard

Registered: 08/04/12
Posts: 9,116
Loc: Canada

Re: AGAR ENVY! (Anything and All things agar!)

[Re: D3_Myc]
#27082252 - 12/10/20 03:54 PM (5 years, 10 months ago)

I can't think of a convincing argument to avoid it other than cost. When making agar or LC you're adding everything necessary for healthy mycelium, plenty of great growers use distilled water exclusively. Eats comes to mind.

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CapMeh
RX Jazzist

Registered: 06/08/09
Posts: 924
Loc: The Holy Mountain
Last seen: 1 year, 2 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Stipe-n Cap]
#27082361 - 12/10/20 04:44 PM (5 years, 10 months ago)

Very good to know thank you for the replies. I assume using distilled water results in clearer agar and might help with my hard water deposits. I’m curious if trace minerals found in tap water are beneficial to have in a dish? Might be insignificant though :shrug:

:shrug:

I’ve only ever used tap water for everything besides making syringes, and I’ve never noticed any problems, not that means there are effects I’m unaware of. I’m sure the region is a variable also.

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Edited by CapMeh (12/10/20 04:49 PM)

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shroomshine
Captain

Registered: 03/11/20
Posts: 247
Loc: off the Richter
Last seen: 3 years, 1 month

Re: AGAR ENVY! (Anything and All things agar!)

[Re: CapMeh]
#27082368 - 12/10/20 04:47 PM (5 years, 10 months ago)

salts are counterproductive

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bang bang free pour gang
Fuck. Oats.

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D3_Myc
Weeb Trash

Registered: 05/06/18
Posts: 4,399
Loc: Year Zero

Re: AGAR ENVY! (Anything and All things agar!)

[Re: shroomshine]
#27082880 - 12/10/20 09:34 PM (5 years, 10 months ago)

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D3’s GlogD3's Agar TEKAgar Porn Gallery RW x MelMak Grow Along Thread

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BSUUF2
derails threads

Registered: 10/15/20
Posts: 666

Loc: not that important
Last seen: 4 years, 7 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: D3_Myc]
#27084557 - 12/11/20 08:50 PM (5 years, 10 months ago)

First time pouring plates (Rusty Whyte clone). Excuse the condensation and bacteria, I'm going to transfer tomorrow...

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LAGM2022

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GrinchGrower
N00B

Registered: 10/02/20
Posts: 682
Last seen: 5 years, 5 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: BSUUF2]
#27084627 - 12/11/20 09:40 PM (5 years, 10 months ago)

Really need to quit making so many transfers... or use one plate for multiples. :iveseenenough:

:iveseenenough:

I have a bad habit of taking 2-3 from every promising plate (with each successive one), so my count is climbing fast

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:minifo:

:minifo:

Trusted Cultivator Methods & Mushroom Cultivation Info :minifo:

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:snowman:

:snowman:

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Sold Out Online
Stranger Danger

Registered: 09/05/20
Posts: 265
Last seen: 2 years, 26 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Clc420]
#27085121 - 12/12/20 08:43 AM (5 years, 10 months ago)

Clc420 said:

I have 2 plates that I'm suspicious about. Would appreciate someone's input. These are t1 from Ms

This happened to a batch of plates I made. Too many nutrients. When I transferred back to lower nutrient plates they were fine.

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Threads that have been useful to me:
Clean Spawn checklist for new growers

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nogoodnamesleft
Stranger than average

Registered: 09/19/20
Posts: 413
Loc: Melmak
Last seen: 2 years, 8 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: CapMeh]
#27085158 - 12/12/20 09:16 AM (5 years, 10 months ago)

CapMeh said:

BTW does anyone know how to prevent the water deposits on the inside of the dish? Would using distilled water fuck with the agar recipe/ myc colonization?

Wait as long as possible to pour , pour in a warm room , leave unwrapped in front of flowhood for 12 hours

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The Fresh Prints
Smell ya later

Registered: 05/19/12
Posts: 1,377
Loc: Bel-Air

Re: AGAR ENVY! (Anything and All things agar!)

[Re: nogoodnamesleft]
#27085218 - 12/12/20 10:17 AM (5 years, 10 months ago)

Running your hood for 12 hours to prevent condensation? Seems like a waste of run-time. 1-2 hours usually does the trick for me. I'm trying to get as much life out of my HEPAs as possible.

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D3_Myc
Weeb Trash

Registered: 05/06/18
Posts: 4,399
Loc: Year Zero

Re: AGAR ENVY! (Anything and All things agar!)

[Re: The Fresh Prints]
#27085238 - 12/12/20 10:31 AM (5 years, 10 months ago)

I do like 3-4hr maybe but I pour as soon as I can physically handle it (135ish) so I have a bit of condensation at first. I’m impatient

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D3’s GlogD3's Agar TEKAgar Porn Gallery RW x MelMak Grow Along Thread

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DutchMyco
Stranger

Registered: 01/09/17
Posts: 284
Last seen: 5 years, 4 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: D3_Myc]
#27085650 - 12/12/20 02:24 PM (5 years, 10 months ago)

Do you change anything for agar media used in slants vs petri dishes?

I made some plates using new agar(gel strength: >1100gr/cm2), 3% MEA (30gr ME/20gr agar/1000ml). But I think it's a bit to thick for plates, will test some 20gr/15gr with this agar next.
I've seen people use thicker, more nutritious agar in slants compared to most people here use in dishes. Does it make any difference at all for long term storage?

Funny thing is, I'm barely seeing contamination on the thick plates, on open air tests. Might suppress spore germination, but also barely bacterial, only after messing with it to get trich over it, it showed many colonies.

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sh4d0ws
LSx

Registered: 02/26/08
Posts: 12,089

Re: AGAR ENVY! (Anything and All things agar!)

[Re: The Fresh Prints]

1
#27085719 - 12/12/20 02:58 PM (5 years, 10 months ago)

Genghis Chron said:

Running your hood for 12 hours to prevent condensation? Seems like a waste of run-time. 1-2 hours usually does the trick for me. I'm trying to get as much life out of my HEPAs as possible.

I find it better to just take plates poured in front of the hood, wrap em in a quick saran wrap covering and then unwrap in a SAB and leave for a few days with the arm holes covered. This eliminates 100% of condensation. Then you don't need to put as much wear on your hepa filter

But if I pour at ~115F condensation isn't really enough of a problem to even bother doing that.

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starbones
I'm an alien, I eat uranium.

Registered: 03/04/20
Posts: 1,131
Last seen: 2 years, 11 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: sh4d0ws]
#27085768 - 12/12/20 03:34 PM (5 years, 10 months ago)

Asked this in the cult general thread but I figure it's better suited to here.

I got two plates from a buddy, PE and APE but it does not appear they survived. Not contaminated but the myc on one plate is extremely fine and stopped growing, the other plate had what looks like a tissue sample that spread the tiniest amount of myc from the transfer then also stopped.

They're about two months old now.

Any hope for these two cultures? Perhaps cut a large chunk of the myc and throw it in sterile water? Perhaps make a very watery agar plate and try to rehydrate in them? I'm sure they're clean so I probably could make some watery agar in some 1/2qt jars for them to sit in and hopefully colonize. I'm not hurting for genetics but these were pre-worked and I'd love to have them in the stable based on what I've seen from their father.

Edited by starbones (12/12/20 04:00 PM)

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Mr. Mushie

Registered: 01/16/20
Posts: 667

Re: AGAR ENVY! (Anything and All things agar!)

[Re: starbones]
#27085850 - 12/12/20 04:12 PM (5 years, 10 months ago)

Most of my plates from the last 75 days, minus ~50 or so.

Agar is fun, glad I started with it.

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Rafiikii

Registered: 11/17/10
Posts: 2,892

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Mr. Mushie]
#27086604 - 12/13/20 04:01 AM (5 years, 10 months ago)

Mr. Mushie said:

Most of my plates from the last 75 days, minus ~50 or so.

Agar is fun, glad I started with it.

just pouring plates for shits and giggles or what?

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"You didn’t come into this world. You came out of it, like a wave from the ocean. You are no stranger here."

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The Dalcassian
Dirty Druggie

Registered: 01/04/17
Posts: 952
Loc: Emerald lsles
Last seen: 3 years, 5 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Rafiikii]
#27086637 - 12/13/20 04:37 AM (5 years, 10 months ago)

Doomsday prep, mush cult style.

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Here Lies This Individual

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Rafiikii

Registered: 11/17/10
Posts: 2,892

Re: AGAR ENVY! (Anything and All things agar!)

[Re: The Dalcassian]
#27086640 - 12/13/20 04:43 AM (5 years, 10 months ago)

The Dalcassian said:

Doomsday prep, mush cult style.

:rofl:

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"You didn’t come into this world. You came out of it, like a wave from the ocean. You are no stranger here."

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jay.ach
Traveler

Registered: 05/25/19
Posts: 538
Loc: USDA Zone 8
Last seen: 1 month, 21 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Rafiikii]
#27086779 - 12/13/20 08:13 AM (5 years, 10 months ago)

I am having a rash of round contams that turn green after a couple of days -- maybe aspergillus? It's not showing in my control plates, so I'm assuming it something I'm doing wrong in the SAB during the transfer. First it was one plate, then a few and on my last round of transfers it was 6 plates.

Anyway, can I successfully transfer from a plate w/ these contams after the green has shown up? Like take a sample of the myc from the side farthest away from the contam?

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“One's destination is never a place, but a new way of seeing things.” - Henry Miller

LAGM v. 2.023
LAGM2021

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Wall.E
Bacteria's Bitch

Registered: 06/05/20
Posts: 3,075
Loc: The Axiom

Re: AGAR ENVY! (Anything and All things agar!)

[Re: jay.ach]

1
#27086807 - 12/13/20 08:35 AM (5 years, 10 months ago)

I'd think it's trich before aspergillus. Isn't that black?

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Mehhh

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roarkell
The Roar Master!

Registered: 08/29/19
Posts: 2,223
Loc: Southern WI

Last seen: 2 years, 6 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: jay.ach]

1
#27086989 - 12/13/20 11:15 AM (5 years, 10 months ago)

jay.ach said:

I am having a rash of round contams that turn green after a couple of days -- maybe aspergillus? It's not showing in my control plates, so I'm assuming it something I'm doing wrong in the SAB during the transfer. First it was one plate, then a few and on my last round of transfers it was 6 plates.

Anyway, can I successfully transfer from a plate w/ these contams after the green has shown up? Like take a sample of the myc from the side farthest away from the contam?

Yes. Thats pretty standard practice for trying to rescue a culture... just move extra slow and be on top of your game... ive been spoiled with my hood... had a nice looking plate show some green and went to save it in the ol SAB and boy, have I gotten sloppy! Lol

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Shop:

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