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Re: Mycelium on Agar Picture Thread [Re: Ferather]
    #26162695 -

Gotcha. Thnx for clearing that up for me.

Ok, at this point i pretty much have to start all the way back from a stack of clean masters. Will get some light malt extract seeing as how most all recipes use it. Gotta make a mass of plates and let them sit for two weeks to make damn sure they're sterile.

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Re: Mycelium on Agar Picture Thread [Re: Feasoghorm]
    #26163383 -

T-Gel without additives is quite hardy, I intentionally exposed a few plates to air for a few days, then I added the lid.
So far the only thing T-Gel germinates from air exposure is a few types of mold and other fungi (wild).

I've yet to see yeast or bacteria germinate, though some bacteria can be transferred.
Spore work should be done on another media-recipe, then transferred.

T-Gel is pro-mycelial in the way that nothing else seems to grow on it, (bacteria and yeast).

----

Note:

T-Gel plain (no additives) contains no sugar sources (no sugar, no starch, no cellulose).
Mycelium must decay and convert the tea phenols into sugar and other.

Example sugar making process: Formose reaction


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Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

DTS DCH Driver for Realtek [DTS:X] - Unlocked and reprogrammed.

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Re: Mycelium on Agar Picture Thread [Re: Ferather]
    #26163413 -

You can make great use of multi spores, multi strains, multi genetics (which ever term you like).

If you use a media that contains the materials from the substrate you intend to fruit from, you will get 'gene expression' (strains), that do well with those materials (and pH, so on).
For example, if my fruiting media was a mixture of grain and coir, my agar media (for isolation) should contain these materials (where possible).

It's possible to boil coir and use the water, or, flour the coir with some spawn grain together (grinder).
You can also add small amounts of course coir to grain jars, (various benefits).

----

Note:

It's more ideal to get gene expression and a phenotype with an oxygen level you also intend to fruit at, (monotub oxygen level).
The more oxygen the mycelium has, the more it's able to decay and produce materials, (including antioxidants).

----

The result should be a strain or strains that are very well suited to it's media and environment.


--------------------
                   

Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

DTS DCH Driver for Realtek [DTS:X] - Unlocked and reprogrammed.

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Re: Mycelium on Agar Picture Thread [Re: Ferather] * 2
    #26163554 -

There's PMs for this. This is the mycelium on agar picture thread not the tea agar info thread.


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Welcome to the Advanced Mycology Forum

Bod's Library    Article behind a paywall? I can try to get it for you

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Re: Mycelium on Agar Picture Thread [Re: bodhisatta] * 1
    #26166735 -

Azzy bridging the gap after taking a wedge. The strands of myc are aerial.



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Shrooms are always bluer on the other side...

Work hard, play harder, trip the hardest.

Simple Cloning TEK by Adas
Semi-Sterile TEK (SS-TEK)
Growing Panaeolus bisporus - The Low-Tech approach
Exotic Mycelia Surviving Winter - Creating IceBreaker Variants!
My First Panaeolus Cyanescens Grow!
Psilocybe azurescens photo compilation 2020
Azurescens in June!

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Re: Mycelium on Agar Picture Thread [Re: Adas] * 2
    #26171820 -

Just in case anyone ever wondered what Trametes betulina myc looks like on Agar. Couldn't find any pics even on Google. You're welcome.



--------------------
Shrooms are always bluer on the other side...

Work hard, play harder, trip the hardest.

Simple Cloning TEK by Adas
Semi-Sterile TEK (SS-TEK)
Growing Panaeolus bisporus - The Low-Tech approach
Exotic Mycelia Surviving Winter - Creating IceBreaker Variants!
My First Panaeolus Cyanescens Grow!
Psilocybe azurescens photo compilation 2020
Azurescens in June!

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Re: Mycelium on Agar Picture Thread [Re: Adas]
    #26172283 -

I thought about it and looked at some of my pours while thinking about it.

My agar does come out very wet on top. Sitting in the sab for a night or two without being sealed can make them dry up.

Should be typical of agar though is to be moist ontop its just a wet type of substrate like jello. The reason its probably so noticeable is the difference between the organic shaping vs looking at mea all the time that is flat as well as clear.

My plates also last very long sealed as well I noticed I still have some from over a year ago doing fine.

Mushroom type above same type of plates below them, mica is a swab plate, bernardi is a new clone on top of a spore plate transfer, with squarrosa I have all white myc and one with yellows a mushroom similar to terrestris does the same thing but instead of orange it browns.

Edited by SSA (09/06/19 12:55 PM)

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Re: Mycelium on Agar Picture Thread [Re: SSA] * 7
    #26226899 -

Inonotus hispidus (medicinal mushroom)

https://www.ncbi.nlm.nih.gov/pubmed/?term=inonotus+hispidus




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Edited by Sporulator (10/03/19 03:20 PM)

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Re: Mycelium on Agar Picture Thread [Re: Sporulator]
    #26244113 -

Gymnopilus purpuratus kicking Rhodotorula's ass. The colony started out orange-red, the myc turned it completely white.



--------------------
Shrooms are always bluer on the other side...

Work hard, play harder, trip the hardest.

Simple Cloning TEK by Adas
Semi-Sterile TEK (SS-TEK)
Growing Panaeolus bisporus - The Low-Tech approach
Exotic Mycelia Surviving Winter - Creating IceBreaker Variants!
My First Panaeolus Cyanescens Grow!
Psilocybe azurescens photo compilation 2020
Azurescens in June!

Extras: Filter Print Post Top
Re: Mycelium on Agar Picture Thread [Re: Adas] * 1
    #26252337 -

Cool thread

Texas Orange Cap, first transfer from spore syringe



Argentina, many transfers from spore print, always fuzzy, thick growth like this...

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Re: Mycelium on Agar Picture Thread [Re: replyom]
    #26265271 -

Does healthy mycelium have rings in it as it grows out on a dish?
Or is it contaminated with acremonium

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Re: Mycelium on Agar Picture Thread [Re: spore-ty]
    #26266622 -

Rings form from either temperature or lighting changes, somebody remind me which.


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Mycelium on agar |  Let's trade

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Re: Mycelium on Agar Picture Thread [Re: bacillus] * 1
    #26266727 -

I get it with temperature changes, without any light changes.


--------------------
                   

Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

DTS DCH Driver for Realtek [DTS:X] - Unlocked and reprogrammed.

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Re: Mycelium on Agar Picture Thread [Re: Ferather] * 1
    #26278733 -

m changing my recipe to a harder agar mix as these rings need to be checked as ive never had anything like it before.  These are 4 different cultures.



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Re: Mycelium on Agar Picture Thread [Re: Edmunter] * 1
    #26295886 -

You call these rings? oO :P

Allenii, alenii, alutacea.



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Mycelium on agar |  Let's trade

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Re: Mycelium on Agar Picture Thread [Re: bacillus] * 2
    #26302495 -



Some melmac on Agar


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I like agar. And eating things. Sometimes i eat agar.

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Re: Mycelium on Agar Picture Thread [Re: Why_celium] * 1
    #26303132 -

Tarragon oyster wood peg to T-Gel + flour, no sterilization:

   

   

Amazing cellulose decay.


--------------------
                   

Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

DTS DCH Driver for Realtek [DTS:X] - Unlocked and reprogrammed.

Extras: Filter Print Post Top
Re: Mycelium on Agar Picture Thread [Re: Ferather]
    #26308750 -

       


--------------------
                   

Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

DTS DCH Driver for Realtek [DTS:X] - Unlocked and reprogrammed.

Extras: Filter Print Post Top
Re: Mycelium on Agar Picture Thread [Re: Ferather] * 2
    #26322778 -

PE6 agar pin put on new agar plate


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Re: Mycelium on Agar Picture Thread [Re: Hobbit GDF] * 3
    #26325294 -


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Mushrooms, Mycology and Psychedelics > Advanced Mycology
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