Home | Community | Message Board


The Spore Depot
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop for: Agar, Brown Rice Flour, Petri Dish

Jump to first unread post. Pages: 1
InvisibleJoshua
Holoman
Male

Registered: 10/27/98
Posts: 5,389
Loc: The Matrix
Agar...what next? *pics*
    #541960 - 02/06/02 02:19 AM (14 years, 9 months ago)

To start off with: for some of you this is a general cultivating question. However, I feel that agar culturing is an advance technique, that is why I am posting in this thread.


I have recently innoculated several agar plates w/ a multispore innoculation. I have fluffy mycelium as shown in the pics.

I am inquiring on my next course of action. I see no rhizomorphic mycelium on my plates. Should I transfer wedges to grain? or should I continue with a possible isolation? This is my first successful attempt at agar culturing and I am in need of advice.

In the future what would be my best course of action for culturing strong mycelium.
I could fruit from BRF and clone from there.
I could repeat my current process.
What other methods work well and will not waste my time?





Joshua


--------------------
The Shroomery Bookstore

Great books for inquiring minds!

"Life After Death is Saprophytic!"


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineAnnoA
Experimenter
 User Gallery

Folding@home Statistics
Registered: 06/18/99
Posts: 24,121
Loc: my room
Last seen: 2 months, 9 days
Re: Agar...what next? *pics* [Re: Joshua]
    #542006 - 02/06/02 02:58 AM (14 years, 9 months ago)

Transfer a few small wedges from each dish to new plates. Then the growth should sector.

I allways go the "frut them once from multispore then clone a good looking fruit" path.


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblealtarego
member
Registered: 10/25/01
Posts: 130
Re: Agar...what next? *pics* [Re: Joshua]
    #542302 - 02/06/02 11:10 AM (14 years, 9 months ago)

Early plates are often too dense with strains to see secotring. Transfer very small wedges and the differences should become obvious.

I agree with Anno that cloning makes it much easier to get a pure strain, though it is fun to see strains emerge from the chaos of a plate.

See my new post for a followup question on this one.

- AE


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineSuntzu
Geek
 User Gallery

Registered: 10/15/99
Posts: 1,394
Last seen: 27 days, 12 hours
Re: Agar...what next? *pics* [Re: Joshua]
    #542332 - 02/06/02 12:11 PM (14 years, 9 months ago)

Hey Joshua, welcome to 'advanced' :smile:  I've read your other stuff and I like your spirit.

As usual, Anno is right. 

Here's how I think about it. . .in that mottled mat of growth you have many many individual genetic combinations.  Some non-mated/un-mate-able growth, some mated hyphae that lack clamp connections [but still might look like decent growth].  Those boundary zones are a good indication of multiple crossings. 
Visualize hundreds of these potentials on a single multispore agar inoculation.  If you put a chunk to grain or a chunk to agar, either way you've begun the process of weeding out the slower growing ones and if grain, the ones not capable of competing on the 'new' food source. . .whittling down the number to the ?couple/few? combos that are represented in the flush.  With this species though, you'll usually get just fine results--no reason why you can't subculture to grain and agar!  The whole 'rhizomorphic' thing is based on the observation that such growth is usually more aggressive and pins well; and if pure, will tend to pin more uniformly if cased/initiated right. 
I've had senesced mycelium that was still rhizomorphic, though.  Also, might be the generation, but the ecuador print I've used always gives fuzzy mycelium. . .even after repeated transfers, though it fruits admirably.  Along the same lines, clones from fruits may not be as rhizomorphic as you'd think. . .at least on first transfer.

The way to do it, IMO, is to keep track of your cultures.  When you get a nice flush, clone one or two.  The more flushes you see, the more you'll be able to choose from.  If possible, make slants or water stocks [easy] for LT storage; this gives you a library to go back to after observing a given flush.  Just remember that unless it's pure, the mycelium growth will go through the selection process again upon the next expansion.  Which could be a good thing, depending.
It's nice to have multispore growth in LT storage, though; you can basically go years without germinating spores.

One of the nicest strains I ever had was taken off a PF cake.  I'm pretty sure it was pure; Ever notice how sometimes on a cake you'll get patches of very rhizo growth?  Pair of tweezers, a lighter. . .         


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineAnnoA
Experimenter
 User Gallery

Folding@home Statistics
Registered: 06/18/99
Posts: 24,121
Loc: my room
Last seen: 2 months, 9 days
Re: Agar...what next? *pics* [Re: Suntzu]
    #542489 - 02/06/02 02:32 PM (14 years, 9 months ago)

>Pair of tweezers, a lighter. . .

Hehe,  that?s exactly how I selected  the culture for these Mazatapec. :wink:

 


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineJuSMe13
newbie
Registered: 08/29/01
Posts: 25
Loc: california
Last seen: 14 years, 8 months
Re: Agar...what next? *pics* [Re: Anno]
    #542582 - 02/06/02 04:01 PM (14 years, 9 months ago)

Wow, I would love a print of those Babies!
very impressive


--------------------
Yo Quiero FSR !


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineSeussA
Error: divide byzero

Folding@home Statistics
Registered: 04/27/01
Posts: 23,480
Loc: Caribbean
Last seen: 22 days, 16 hours
Re: Agar...what next? *pics* [Re: Suntzu]
    #542659 - 02/06/02 05:10 PM (14 years, 9 months ago)

Nice info, thanks! What are the benefits of slants vs petri dishes for long term storage?


--------------------
Just another spore in the wind.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineSuntzu
Geek
 User Gallery

Registered: 10/15/99
Posts: 1,394
Last seen: 27 days, 12 hours
Re: Agar...what next? *pics* [Re: Seuss]
    #543720 - 02/07/02 02:58 PM (14 years, 9 months ago)

Don't have a lot of time now, but here's a water culture pic. . .the growth is only a couple days;



I will never go back to slants after using water cultures, they are simpler to make and according to the references will last longer. If you use slant-type tubes, storage space is the same for both. I think the key is getting as little agar as possible in the suspension; less opportunity for contams and the fungi don't seem to mind. I also find it easier to remove a few drops of the water suspension rather than try to pick out some growth off of a slant [or dig through mineral oil. . .ugh].


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineAnnoA
Experimenter
 User Gallery

Folding@home Statistics
Registered: 06/18/99
Posts: 24,121
Loc: my room
Last seen: 2 months, 9 days
Re: Agar...what next? *pics* [Re: Suntzu]
    #543861 - 02/07/02 05:16 PM (14 years, 9 months ago)

>according to the references will last longer

The reference:

http://www.mycoculture.org/ftpdir/culture-storage-technique


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop for: Agar, Brown Rice Flour, Petri Dish

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

Similar ThreadsPosterViewsRepliesLast post
* Amazon agar pic katfish 1,054 2 12/30/07 11:26 PM
by katfish
* Rhizomorphic and "cottony" mycelia Anonymous 5,289 16 03/30/04 08:37 PM
by Anonymous
* Problems getting Rhizo growth from MEA
( 1 2 all )
upupup 3,060 32 12/05/02 08:44 AM
by stonErollEr1
* Multispore vs. clone & woodlovers question altarego 1,076 5 02/09/02 08:22 PM
by altarego
* Some proven agar nutrient media recipes Una 4,911 11 10/03/04 03:15 PM
by YSr
* Re: Ecuador strain on agar picture, is it decent or not? Anonymous 771 7 02/15/00 11:32 PM
by Anonymous
* Agar transfer question Dex_Detrimental 814 3 06/12/02 07:51 PM
by Dex_Detrimental
* Agar times xburn 903 5 05/11/05 05:29 PM
by X24

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Prisoner#1, RogerRabbit, EvilMushroom666
1,869 topic views. 1 members, 8 guests and 0 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Edabea
Please support our sponsors.

Copyright 1997-2016 Mind Media. Some rights reserved.

Generated in 0.053 seconds spending 0.004 seconds on 14 queries.