verum subsequentis said:

Those plates are indeed looking grand. You'll want to start taking smaller transfers though. Big chunks like that just up your chances of not isolating away from something nasty. Other than that, you're looking super fine.

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ok cool, I thought if the agar cuts were bigger it would have more surface area to work with increasing the speed of which the myc grows.  would I use that large of a size of cut to do the A2G transfers? Or never under any circumstance should I use cuts that big?


Also, should I just plan to toss out the other 5 initial clone tissue plates as I now have clean transfers to the new ones?  only reason I havent yet is cause I wasnt sure which type of growth would be better for potential finished weight... the white fluffy type or the stringy type?  I guess I would have to just innoc some jars up to see what the actual results would be huh?  Planning on doing 10 or so 66 qt tubs here soon as my master jars are good to go .

Stephie said:

Sorry for this not so helpful comment, but to clarify, mold has mycelium as well. It just sporulates on the mycelium instead of creating large fruiting structures.

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mushman1017 said:

Stephie said:

Sorry for this not so helpful comment, but to clarify, mold has mycelium as well. It just sporulates on the mycelium instead of creating large fruiting structures.

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Do you think thats what is happening on my first 3 pictures?

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Those 3 do indeed look like mold.

The first F+ one has a spot that looks like bacteria (or perhaps yeast). The second F+ I don't see anything in.

I'm not really joking when I say my vision is shite though, so if anyone sees something different, please let it be known

mushman1017 said:

Ok cool, I thought if the agar cuts were bigger it would have more surface area to work with increasing the speed of which the myc grows.  would I use that large of a size of cut to do the A2G transfers? Or never under any circumstance should I use cuts that big?

Agar isn't really about generating mycelium quickly. We use agar to isolate clean growth away from bacteria, yeast, mold... all the evil shit. This is why we take small transfers. Often times the noob believes he can positively visually identify a clean plate but isn't trained well enough yet to notice the very sneaky bacteria riding along in the culture.  Taking nice small transfers from the best looking areas is the best bet.

When transferring into a grain jar you use more. This is because you've (hopefully) already done the transfers needed to attain a clean culture on agar. You can use the whole plate or cut it into pizza slices and noc up a bunch of jars. You can do as many jars as you want, really. But speed is helpful when colonizing grain. I'd recommend a plate to four quarts as a good starting place.

Also, should I just plan to toss out the other 5 initial clone tissue plates as I now have clean transfers to the new ones?  only reason I havent yet is cause I wasnt sure which type of growth would be better for potential finished weight... the white fluffy type or the stringy type?  I guess I would have to just innoc some jars up to see what the actual results would be huh?  Planning on doing 10 or so 66 qt tubs here soon as my master jars are good to go .

If they are clean you can save them and look for pins to form. These are nice for easily getting clones that usually do alright. Or you can make up some more jars and throw them in there. Or you can trash them. Or you can leave them laying around for your observation. It's a good idea to observe a lot. If you really want to get good at something, the best thing to do is spend a lot of time with it. Just watching old plates grow will teach you a lot of things. Like, for instance, why many people fear the edges of the dish and most recommend not using the edges .

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verum subsequentis said:

mushman1017 said:

Taking nice small transfers from the best looking areas is the best bet.



Just watching old plates grow will teach you a lot of things. Like, for instance, why many people fear the edges of the dish and most recommend not using the edges .

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So what determines a "best looking area"? The black, red, or yellow circled areas? I still dont know what to look for other than what seems to grow and expand out the fastest...



Also notice the color of the center piece inside the blue? That was obviously the original agar I transferred from.  Is it a little mis colored cause I flipped it upside down and the myc covered it and made it look weird? Or is that possible bacteria?

I will prob only use 4 of the 8 dishes that are growing out now to inoculate 6 jars I have as masters, then will use those for 60 jars after. The other 4 dishes should I wrap with the inch of cling wrap and throw in zip locks and in the fridge to hold out for a later time? Should I wait for them to grow out to the edge to get the most out of it or make sure to throw it in the fridge before it ever touches the sides?

mushman1017 said:

So what determines a "best looking area"? The black, red, or yellow circled areas? I still dont know what to look for other than what seems to grow and expand out the fastest...

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