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Re: I seriously don't get it. [Re: 10kVisions] * 1
    #26011525 -

You shouldn't wipe your exacto after flaming it.

The torch sterilizes it. No need for wipe.


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JJ

Draw unto others as they have been drawn to you.... WSP

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Re: I seriously don't get it. [Re: 10kVisions]
    #26011530 -

:whathesaid: Cool the blade in the adjacent agar before cutting the wedge.

You may have a higher spore load in the new space and the nasty could be crashing the party during inoculation. You could try making some grain petri’s with about 1/4 inch of grains in the bottom of wide mouth pints or pint ziplocks. Drop a wedge/ms solution in the middle and let it grow out without shaking. If satellite growth pops up anywhere else, you’ll know the contam is coming in when you are introducing the myc.


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LAGM 2.022

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Re: I seriously don't get it. [Re: ttching8475]
    #26011562 -

and another thing.  I tried to do a tek exactly how a close friend is doing it (he came an helped) we did it step by step exactly the same wya he does it and did it 3 days before me.  he got 7 successful tubs and I got mold in jars.

AND he knocks straight from syringes. no agar.  so this is all very confusing to me.  I can't seem to find anyone to give me definitive answers. only guesses and opinions and even after doing what the opinions and guess are I get the same result... can my ENTIRE room be contaminated? (no one answered that)


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Re: I seriously don't get it. [Re: 10kVisions]
    #26011564 -

No it's the way you are doing things ... those plates you have are funky but you also are using a huge wedge to transfer.. we dont know how your grain prep is .. if you're using a still air box flow hood .. how you sterilize your tools ect.


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Re: I seriously don't get it. [Re: Boogieman47]
    #26011571 -

Boogieman47 said:
No it's the way you are doing things ... those plates you have are funky but you also are using a huge wedge to transfer.. we dont know how your grain prep is .. if you're using a still air box flow hood .. how you sterilize your tools ect.


I covered all this already.... read the thread


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Re: I seriously don't get it. [Re: 10kVisions]
    #26011578 -

Try doing agar with something other than mason jars for starters.


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Re: I seriously don't get it. [Re: 10kVisions]
    #26011581 -

Only thing I can tell you is get you agar looking cleaner ... be extra clean and sterile .. things will get better .. if you want to use straight spores to grain no one is stopping you ... at all


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Re: I seriously don't get it. [Re: bodhisatta]
    #26011612 -

bodhisatta said:
Try doing agar with something other than mason jars for starters.


They’re not pint jars Bod.  I’m using pasty whites agar Tek. But instead of the plastic small containers I use the ball glass version. Same size etc just glass instead of plastic.


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Re: I seriously don't get it. [Re: 10kVisions]
    #26011617 -

Take a pic of what they look like cause they shouldn't be that hard to cut a piece of rice out of the agar


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Re: I seriously don't get it. [Re: 10kVisions]
    #26011635 -

If you wipe with alcohol AFTER torching it, it's sanitised, not sterilised

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Re: I seriously don't get it. [Re: UncleHoyphae]
    #26011648 -

I thought your cultures looked a bit iffy....could just be the size and placement of the xfers but could definitely look more uniform.  As mentioned, try grabbing smaller samples and place them in the center of the receiving plate.  Also, what’s the filter setup on your spawn jars?


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Helpful Threads
stonesun
Mycolorado’s Tamp Fruit Project
Tampanensis Grow with Mycolorado
bw86's Jalisco Grow

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Re: I seriously don't get it. [Re: Mycolorado]
    #26011669 -

EDIT: Apparently I missed the whole 2nd page of the conversation...  :foreheadslap:  My bad, Ignore

Quote:
wiping it with alcohol after torched


This is bad, you just unsterilized your sterile blade by sanitizing it. Look up the difference between sterile and sanitize. And because you use such large pieces of agar, even though it looks clean, it might be hiding contams in the disorganized growth. Use smaller wedges and wait for clean and organized growth to be sure.


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First Grow

Edited by Raccoon (05/24/19 08:08 PM)

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Re: I seriously don't get it. [Re: Raccoon]
    #26011679 -

Raccoon said:
EDIT: Apparently I missed the whole 2nd page of the conversation...  :foreheadslap:  My bad, Ignore

Quote:
wiping it with alcohol after torched


This is bad, you just unsterilized your sterile blade by sanitizing it. Look up the difference between sterile and sanitize. And because you use such large pieces of agar, even though it looks clean, it might be hiding contams in the disorganized growth. Use smaller wedges and wait for clean and organized growth to be sure.


Ok that makes sense.  I honestly didn’t know that (lesson learned) I’ll be knocking new agar tonight and I’ll start a new thread for a step by step for people to watch.

Can anyone tell me if PC’ing at 19PSI for 2hours is enough for sterilization at 10k feet?

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Re: I seriously don't get it. [Re: 10kVisions]
    #26011699 -

10kVisions said:
bodhisatta said:
Try doing agar with something other than mason jars for starters.


They’re not pint jars Bod.  I’m using pasty whites agar Tek. But instead of the plastic small containers I use the ball glass version. Same size etc just glass instead of plastic.


yea I know.

19 for 2 should be just fine at 10k


--------------------
Everything you need in one spot.

My Q&A thread. Ask me questions here rather than in Private Message
Bod's chat thread

5g a day keeps the 5g away

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Re: I seriously don't get it. [Re: bodhisatta]
    #26011843 -

bodhisatta said:
10kVisions said:
bodhisatta said:
Try doing agar with something other than mason jars for starters.


They’re not pint jars Bod.  I’m using pasty whites agar Tek. But instead of the plastic small containers I use the ball glass version. Same size etc just glass instead of plastic.


yea I know.

19 for 2 should be just fine at 10k



Thanks.  Let me ask you this.  How would I reheat an agar bottle with no microwave?  Most people make a bottle, sterilize it and then pour each Petri dish then to re heat they use a microwave. I don’t have a microwave.  I do pasty whites tek because I can make the jars and then sterilize them


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Re: I seriously don't get it. [Re: 10kVisions]
    #26011860 -

10kVisions said:
bodhisatta said:
10kVisions said:
bodhisatta said:
Try doing agar with something other than mason jars for starters.


They’re not pint jars Bod.  I’m using pasty whites agar Tek. But instead of the plastic small containers I use the ball glass version. Same size etc just glass instead of plastic.


yea I know.

19 for 2 should be just fine at 10k



Thanks.  Let me ask you this.  How would I reheat an agar bottle with no microwave?  Most people make a bottle, sterilize it and then pour each Petri dish then to re heat they use a microwave. I don’t have a microwave.  I do pasty whites tek because I can make the jars and then sterilize them


Hot water bath on the stove

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Re: I seriously don't get it. [Re: UncleHoyphae]
    #26011899 -

UncleHoyphae said:
Hot water bath on the stove



thats what i was thinking, guess i just need confirmation. thanks


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Re: I seriously don't get it. [Re: 10kVisions] * 1
    #26011941 -

I also agree that you need to be making small transfers. You can pick up a small wedge really easy, just stab it at a 45 degree angle and lift straight up. Like this:


When you are putting it into the receiving plate, cut into the fresh agar and cut through it. This will get the wedge to slide off. Like this:

It's actually stupid easy when you get used to it.

Can I ask if you have pics of the spawn jars before going into tubs?


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Re: I seriously don't get it. [Re: Mad Season]
    #26012220 -

do not wipe scalpel with alcohol after torching.

torch->cut wedge->transfer->wipe scalpel alcohol-> repeat

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Re: I seriously don't get it. [Re: Mycolorado]
    #26012294 -

:whathesaid:
Mycolorado said:
what’s the filter setup on your spawn jars?



--------------------
🅂🄸🄶🄼🄰


Helpful Threads
stonesun
Mycolorado’s Tamp Fruit Project
Tampanensis Grow with Mycolorado
bw86's Jalisco Grow

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