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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Spots on petri dishes. pls help!
    #25891247 -

First time growing and I have inoculated petri dish with GT spore syringe on 15th March 2019.7 days have passed and I can see spots on places where I have inoculated but no mycellium. Is this ok? What to do?






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Re: Spots on petri dishes. pls help! [Re: mazhary]
    #25891259 -

Contamination of some sort

You might be able to identify it from pictures here; the how it should & shouldn't look thread by tmethyl

https://www.shroomery.org/forums/showflat.php/Number/17231313#17231313


...maybe?



Baccilus bacteria

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Re: Spots on petri dishes. pls help! [Re: sh4d0ws]
    #25891272 -

but it's on exact same places where i dropped spores. if yeast contamination, shouldn't it have been obvious on petri dish prior to inoculation?

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Re: Spots on petri dishes. pls help! [Re: mazhary]
    #25891294 -

Looks a little funny. With spores I do a few dishes at once, they have bunches of contams.


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Re: Spots on petri dishes. pls help! [Re: mazhary]
    #25891348 -

mazhary said:
but it's on exact same places where i dropped spores. if yeast contamination, shouldn't it have been obvious on petri dish prior to inoculation?


Your spore syringe likely had the contaminant inside of it, especially if that's where the spots showed up


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Re: Spots on petri dishes. pls help! [Re: sh4d0ws]
    #25891356 -

Mmm bacteria yummy


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The thing about deceit is, you don't know your being deceived...

The only thing I am sure about is that I know nothing - socrates

Believe nothing you hear and half of what u see.

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Re: Spots on petri dishes. pls help! [Re: MoxieSteadman]
    #25891361 -

mazhary said:
but it's on exact same places where i dropped spores. if yeast contamination, shouldn't it have been obvious on petri dish prior to inoculation?



When opened to the air it can come from anyway where. Bacteria I literally everywhere. Could have been from ur glove, the air, the needle, the out side of the syringe, :shrug: who knows.

MoxieSteadman said:
Looks a little funny. With spores I do a few dishes at once, they have bunches of contams.


Try pasty plates! They are so much easier imo! Once u get those down u can try to tackle petris again. Since the pasty plates have high sides they are less likely to get contaminated when u open them in your SAB. I perfer pasty plates so I don't have to buy new petri dishes all the time...


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The thing about deceit is, you don't know your being deceived...

The only thing I am sure about is that I know nothing - socrates

Believe nothing you hear and half of what u see.

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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