Home | Community | Message Board
You are not signed in.
Sign In New Account
Forum Index Search Posts Trusted Vendors Highlights Galleries FAQ User List Chat Store Random Growery

Sporeworks
This site includes paid links. Please support our sponsors.

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: North Spore Injection Grain Bag   Sporeworks.EU Spores for European Microscopy   Mushroom-Hut Liquid Cultures   Original Sensible Seeds Bulk Cannabis Seeds

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
hows it lookin' * 1
    #25857365 -

Started my hand at agar.  Did MEA.  These are my first pours.  I used a MS syringe.  I tried to inoculate 10 plates and only had one grow ANYTHING.  I think i did not let my needle cool enough after flame and only put one drop on each plate.  One drop seemed like a lot of liquid but i think i cooked most of it.  :foreheadslap:

here is my only survivor.  he looks pretty damn clean and isolated to me.  Is there value in transferring or should i just let him grow out and use for grain inoculation.  Seems like transferring him would just get it to the center of the plate.  I know it is t word for fluffy growth but i know that does not matter.

thanks for help


Extras: Filter Print Post Top
Re: hows it lookin' [Re: lost wanderer]
    #25857370 -

Looks good you need to make a transfer from the leading edge. You should make atleast 2 transfers to make sure you get a clean plate.


--------------------
How I make and preserve tea


Consuming consumes a man That was never a purpose of life To only crave for material joys Is believing the lie - Mellow Mood

Extras: Filter Print Post Top
Re: hows it lookin' [Re: SFS96]
    #25857404 -

Ready to transfer, looks pretty uniform. You can take that out to ten or more plates if you like. Yeah at least a few transfers is best to ensure there are no hidden contaminants. Other than that the benefits are that you will be narrowing down the genetics, which is desirable, and you can turn that one plate into as many as you want. Then you can put 10 or 20 (+) agar wedges to grain, rather than just 1 or 2 from that plate.

Btw, isolate is usually used in terms of strains, probably not what you mean here.

Oh and when I do MS solution to agar, I like to let a drop out onto the floor of the SAB after flame sterilizing, to cool the needle.


--------------------
To avoid all evil, to cultivate good, and to cleanse one's mind - this is the teaching of the Buddhas

Edited by juniperus (03/06/19 11:27 PM)

Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: North Spore Injection Grain Bag   Sporeworks.EU Spores for European Microscopy   Mushroom-Hut Liquid Cultures   Original Sensible Seeds Bulk Cannabis Seeds

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Similar ThreadsPosterViewsRepliesLast post
* transferring mycelium wikedanjel 4,407 13 01/10/03 03:54 AM
by debianlinux
* G2G Transfer? lvleph 4,634 9 11/20/02 12:28 PM
by scifi5001
* substrate transfer?!?! dets 3,084 8 11/13/02 12:29 AM
by SixTango
* G2G transfers? lvleph 4,378 15 11/03/13 06:15 AM
by SpitballJedi
* Transferring from myco-bag to terrarium? how? axtv 1,528 6 10/01/02 09:40 AM
by Skikid16
* How hot it is in the steam pot ?
( 1 2 3 all )		 michaz 5,696 58 09/24/02 02:52 AM
by djamor
* how much cc to use on quart jars. philipkenobi 3,346 1 11/07/01 02:04 AM
by NDK
* How to Inoculate PF jars with mycelium? OJ 2,323 10 12/10/02 08:30 AM
by Audi0

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, the_chosen_one, LogicaL Chaos, 13shrooms, hamloaf, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
75 topic views. 19 members, 3,429 guests and 146 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2026 Mind Media. Some rights reserved.

Generated in 0.029 seconds spending 0.012 seconds on 16 queries.