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Re: why stop at 3rd generation in gtg transfer [Re: medical]
    #25742340 -

I agree but won't spores be a totally different set of genetics?

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Re: why stop at 3rd generation in gtg transfer [Re: Funguyadventures]
    #25742350 -

Not only will they last, but you don't have to worry  about agar drying out, constantly doing transfers. May come a point in the future where you are just unable to have living cultures, or a PC, or your fridge dies, power goes out, whatever the reason, prints are they way.

Yes, you would have to start from scratch isolating, cloning etc, however, you have the strain, like your original work. Some one here, just germinated spores that we're 15 some years old, and he mentioned he wasn't over anal about taking care of them, think he found them in box on his basement or perhaps even his attic. The spores will contain the same gentic pool.

Edited by Tstone (01/13/19 05:14 PM)

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Re: why stop at 3rd generation in gtg transfer [Re: Tstone]
    #25742357 -

Well damn. I would say that the best way is to diversify, grow out and collect new prints annually in addition to keeping cultures.

Now, I have a few prints from 2011 I should dig up...

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Re: why stop at 3rd generation in gtg transfer [Re: medical]
    #25742364 -

Prints last years and years. So do spore syringes. 5+ years is no issue for spores.

There's edible cultures of shiitake and oysters that have been around more than a few decades. Cultures can last indefinitely with good storage.

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Re: why stop at 3rd generation in gtg transfer [Re: medical]
    #25742365 -

With older spores, they should be hydrated in  sterilized water, like in a syringe, for better germination outcomes, prior to using.


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Wake now, discover that you are the song that morning brings, but the heart has it's seasons, it's evenings and songs of it's own"

Edited by Tstone (01/13/19 05:23 PM)

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Re: why stop at 3rd generation in gtg transfer [Re: Tstone]
    #25742434 -

Technically if something happened to your master and any backups you could recreate a master if you cloned in agar from tissue pulled from a mushroom, correct?

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Re: why stop at 3rd generation in gtg transfer [Re: seussiii]
    #25742473 -

Sure, however even clones have hundreds of genetics in them, so when you take clone tissue, you aren't guaranteed to get an exact match as the donor, it just increases the likelihood of similar genetics.

Unfortunately , cloning shrooms aren't as exact as Cloning plants. If your on  your 8th G2G transfer, and clone tissue, your tissue sample is the same age as the the original master jar. When clones are done, most tend to take samples from the first tub / grow made from the master, keeping the mycelium as young as possible, in order to get the most future use from it( regarding health,vigor etc..)

Someone recently mentioned that the clone tissue sample he took, produced small fruits, instead of the large ones like the donor, and cut his yield by 1/2.


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Wake now, discover that you are the song that morning brings, but the heart has it's seasons, it's evenings and songs of it's own"

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Re: why stop at 3rd generation in gtg transfer [Re: bodhisatta]
    #25742492 -

bodhisatta said:
Senescence is something most of won't even be able to juggle a culture long enough to run into.

I think most of that info stems from contaminants and people getting dirty after so many g2gs. Ive been to a couple farms that only order a master culture every couple years and keep it going all that time between

A lot of edible farms are fucking jokes. And a few have their shit together. One mushroom farm I visited didn't do g2g at all they bought all their spawn. They said a lab is too complicated and risky. They went out of business after a year.



:takingnotes:

This is interesting as I was also always under the impression that you did not want to push g2g past 2-3 generations, but then again, so much info has changed since I began cultivation that this was just another lingering detail I had stored in my head with no personal proof.

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Re: why stop at 3rd generation in gtg transfer [Re: Tstone]
    #25742509 -

Tstone said:
Sure, however even clones have hundreds of genetics in them, so when you take clone tissue, you aren't guaranteed to get an exact match as the donor, it just increases the likelihood of similar genetics.

Unfortunately , cloning shrooms aren't as exact as Cloning plants. If your on  your 8th G2G transfer, and clone tissue, your tissue sample is the same age as the the original master jar. When clones are done, most tend to take samples from the first tub / grow made from the master, keeping the mycelium as young as possible, in order to get the most future use from it( regarding health,vigor etc..)

Someone recently mentioned that the clone tissue sample he took, produced small fruits, instead of the large ones like the donor, and cut his yield by 1/2.




Ahh good stuff. Yea that makes sense. Sounds like the process of developing cold/hot hardy varieties of gourmet mushrooms would be a pita.

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Re: why stop at 3rd generation in gtg transfer [Re: seussiii]
    #25742671 -

Do slants
Some people use LC for for long term storage
On a unrelated subject some people drink cat urine and worships the sun god

Don't do LC for long term storage do it the right way, slants


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Nothing breads nothing

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