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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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The Guide. A Psilocybin Cubensis B+ grow log * 8
    #27395010 -

Nothing I am doing here is new/my own, I just wanted for years to put together a post that walks even the newest member through how to be successful and where I am currently with cult.  Thanks to everyone since 2005 who has helped me, RR, Damion, mushboy, munchauzen, Bod, D3, inocuole, tons of people on the boards.



The Guide

on 6/2 I used 2 500mL media bottles and made the recipe 10g agar, 10g LME, and 500mL water and pressure cooked for 30 minutes at 15PSI, everything is done using a SAB

 


6/8 I made plates where I used prepackaged sterile applicators and swabbed a B+ print sent to me in 2015 to 10 plates while snapping the swab off in plate #10.

   

6/18 I then took wedges from plate 10 and transferred to 6 more plates    

I made a third and final set of transfers before moving to jars

Around 7/1 I used Bod's Easy AF Oat prep tek  and placed 8 wedges into jars (this is my favorite way to do grains and I spent a decade using RR's rye berry tek so trust me) https://www.shroomery.org/forums/showflat.php/Number/24126032 ; 



Jars were around 30% colonized on 7/14 and were shook, this was 7/16


This was 7/19


On 7/20 4 of my 8 jars were ready so I broke them up and used 2 jars of spawn to 2 jars of substrate using damion5050's bucket tek( https://www.shroomery.org/forums/showflat.php/Number/11916595#11916595 ) and sterilite shoebox sized containers, something I learned from Munch
 

Here is the original plate #10 today, fruiting a mushroom right from the tip of the applicator, the plate where this all began.


Here is my elaborate grow set up.  2 shoeboxes with a desk lamp and 6500K bulb pointing down at it off the side of my gaming pc.  I put directly into fruiting conditions(basically throw the light on for 12 hours a day :shrug:


The next step is wait for these 4 other jars to colonize this week and continue doing grain to grain transfers as well as keep doing agar transfers so I have tissue at the ready. 

I had time so I did the write up now, I will update when everything starts to fruit.

Fruits 8/7

The 3 tubs did similarly.  I slowly harvested mushrooms off them as soon as their veils tore so this is what is going on currently.

     

Each of the 3 tubs produced 1 ounce dry from first flush.  Dunked all 3 8/12 and will wait for next flushes.


     

Edited by PreparationH (02/21/23 12:21 PM)

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Re: The Guide. A Psilocybin Cubensis B+ grow log [Re: PreparationH] * 1
    #27395025 -

:popcorn:

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Re: The Guide. A Psilocybin Cubensis B+ grow log [Re: Stipe-n Cap]
    #27395988 -

So are G2G transfers or agar wedges easier in the end?¿


--------------------
I never thanked ya fer saving my life.

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Re: The Guide. A Psilocybin Cubensis B+ grow log [Re: Lieutenant Pan] * 1
    #27396070 -

:creepylurker:


--------------------

..:: E V E R Y  ::..

..:: New? Start here. ::..
..:: How I Panaeolus. From Agar to Tea ::..

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Re: The Guide. A Psilocybin Cubensis B+ grow log [Re: Lieutenant Pan]
    #27396197 -

Lieutenant Pan said:
So are G2G transfers or agar wedges easier in the end?¿



I think this is somewhat subjective, and your personal setup and goals will dictate the best direction.

Agar is best if you're starting from spores and want to isolate genetics for a good grow. (Same with cloning an existing grow)

G2G is best to increase the amount of spawn you have without needing to isolate new cultures.

I'm pretty sure G2G is also faster simply due to the amount of inoculation points.

But space is a big consideration. You won't want to g2g 8 jars in an SAB, that would be hell, you could mess with 8 agar dishes in that amount of space though.

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Re: The Guide. A Psilocybin Cubensis B+ grow log [Re: coversall]
    #27396216 -

I love the plate with the pin coming out of the swab!

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Re: The Guide. A Psilocybin Cubensis B+ grow log [Re: PreparationH]
    #27396446 -

I predict some trouble with your spawn there prepH, consider doing a2g and just spawning those, I suspect that g2g'ing those is going to create some issues.

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Re: The Guide. A Psilocybin Cubensis B+ grow log [Re: Stipe-n Cap]
    #27396459 -

What makes you think that?  Smells good, broke up great, made another tub today I think it's golden.

I never put all the eggs in one basket though, I plan to do more wedges anyway


--------------------
My cultivation guide.  How anyone can grow mushrooms. 

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Re: The Guide. A Psilocybin Cubensis B+ grow log [Re: PreparationH]
    #27396465 -

I could be wrong, just based on the pics.  I trust that you got your show under control, I can't help but comment on spawn pics.

Looking forward to seeing your grow.

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Re: The Guide. A Psilocybin Cubensis B+ grow log [Re: Stipe-n Cap]
    #27396479 -

We'll see, regardless I have plates fruiting, more agar to work on, tubs goin, :feelsgoodman:

I made this today while spawning another tub, I'm honestly most excited for this thing lol




--------------------
My cultivation guide.  How anyone can grow mushrooms. 

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Re: The Guide. A Psilocybin Cubensis B+ grow log [Re: PreparationH] * 2
    #27396497 -

:ohyeah: keep it up brotha


--------------------

All of Munch's Teks!
Munch's YouTube Channel

:suckit:Munch's Mushroom Tutorial Videos!:suckit:
Munch's Monotubs with Liners
Blenderless Liquid Inoculate
Agar as Easy as 1-2-3!

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Re: The Guide. A Psilocybin Cubensis B+ grow log [Re: Munchauzen]
    #27396558 -

How many wedges can you get off a plate?  With G2G you can turn 1 jar into 1000's (from what I've seen in the movies). 


--------------------
I never thanked ya fer saving my life.

Edited by Lieutenant Pan (07/21/21 12:20 PM)

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Re: The Guide. A Psilocybin Cubensis B+ grow log [Re: MikeRodose]
    #27398059 -

You can get more than you need off a plate, cutting a wedge the size of a grain of rice gives you tons to work with. 


MikeRodose said:
I love the plate with the pin coming out of the swab!



2 days later: 


--------------------
My cultivation guide.  How anyone can grow mushrooms. 

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Re: The Guide. A Psilocybin Cubensis B+ grow log [Re: PreparationH]
    #27398264 -

:camping:

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Re: The Guide. A Psilocybin Cubensis B+ grow log [Re: stubb]
    #27402883 -

This is the shoebox from 7/20




I assume I will see pins in the next 10 days.


--------------------
My cultivation guide.  How anyone can grow mushrooms. 

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Re: The Guide. A Psilocybin Cubensis B+ grow log [Re: PreparationH] * 2
    #27418416 -

I harvested a good amount of mature mushrooms the last 4 days off the 3 tubs before the photo today,  it turned out great and I have 4 more shoeboxes colonizing too with more B+.










 



--------------------
My cultivation guide.  How anyone can grow mushrooms. 

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Re: The Guide. A Psilocybin Cubensis B+ grow log [Re: PreparationH] * 1
    #27418632 -

Thanks for your detailed posts and continued updates!  I started my first ever B+ / Golden Teach Pf-Tek grow on 6/12 right about when you started this grow, so it's been great to watch and compare progress.  I'm into my second flush now, and getting ok results for cakes, but nothing like this of course!

I'm definitely trying agar and bulk substrate for my second grow.  What seemed daunting 2 months ago now looks like just a another process step, and actually eliminates a lot of hassles.  I already have 2 test plates going from my first-ever spore print.  I cheated and bought pre-poured plates, will learn to do that the right way eventually.  I found a local source of horse oats, cheap! One of the brands shown in BOD's tek.

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Re: The Guide. A Psilocybin Cubensis B+ grow log [Re: Shroomintune]
    #27426052 -



all 3 tubs did well, each one produced 30g dry for the first flush, just dunked them each 4 hours and will see how much more they put out


:vibin:


--------------------
My cultivation guide.  How anyone can grow mushrooms. 

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Re: The Guide. A Psilocybin Cubensis B+ grow log [Re: PreparationH] * 1
    #27486206 -

The run still is not over, I wanted to show my new largest mushroom I have ever grown or even seen, thing is like the side of my forearm, I didn't realize until I started tugging at it that it wrapped around the whole tub!





5.05 grams dry

Edited by PreparationH (09/29/21 03:01 PM)

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Re: The Guide. A Psilocybin Cubensis B+ grow log [Re: PreparationH] * 2
    #27490370 -

Just wanted to say thanks again for the inspiration to move past PF-Tek.  My first 11 (!!!) shoeboxes of Clone->Agar->Oats->Bulk are just starting to pin. Looks like the are going to be AMAZING.  I lost one oat jar to contam, and I suspect it was from the plate I transferred off.  It had a little green spot just starting on the far side of the plate, must have spread it's spores around.  Lesson learned.

I used mostly BOD's Teks for prep, and then ShaperDreaming's Shoebox Tek for spawning, both to the letter.

I'm also running ONE shoebox full of Uncle Ben's, just because I was curious, I had a spare MS syringe with no other plans, and I'm the type of person where if you tell me the stove is hot, I have to touch it and see for myself. I had no expectations, but used the best sterile technique I could.  2 of the 4 UB bags were grossly contamed and were trashed after opening, the other 2 bags were combined with CVG, spawned to a shoebox and are doing well so far. 

But the once you have the equipment, Oat jars and agar are clearly WAY cheaper to run and far more reliable. 50 lbs of oats costs $20 and is enough to make 120 shoeboxes.  17 cents per shoebox. After the contams, took me 4 bags of pre-cooked rice to make one shoebox - $8 per shoebox. Almost 50 times more expensive. 

For other newbs like me to reference, these are the Teks I used:

BOD's SAB Tek: https://www.shroomery.org/forums/showflat.php/Number/23990888
BOD's Agar Tek: https://www.shroomery.org/forums/showflat.php/Number/21922023
BOD's Oat Prep Tek:  https://www.shroomery.org/forums/showflat.php/Number/24126032
Spitball's CVG recipe: https://www.shroomery.org/forums/showflat.php/Number/20469910
BOD's Coir Prep Tek: https://www.shroomery.org/forums/showflat.php/Number/24077162
SFF Shoebox Assembly Tek: https://www.shroomery.org/forums/showflat.php/Number/26009662

Edited by Shroomintune (11/29/21 05:39 PM)

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