Home | Community | Message Board
You are not signed in.
Sign In New Account
Forum Index Search Posts Trusted Vendors Highlights Galleries FAQ User List Chat Store Random Growery

Magic-Mushrooms-Shop.com
This site includes paid links. Please support our sponsors.

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Sporeworks.EU Spores for European Microscopy   North Spore Bulk Substrate   Original Sensible Seeds Bulk Cannabis Seeds   MagicBag.co All-In-One Bags That Don't Suck   Myyco.com Golden Teacher Liquid Culture For Sale   Mushroom-Hut Grow Bags

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Got agar, whats next?
    #25696100 -

Relatively new, enjoying my recent success and trying to build upon it.

So I started growing about 6 months ago, with a failed attempt at brf and a failed try with spore syringe to honey water LC...bad idea I know now.
Since then I have had 6 successful grows with spore syringe to brf cakes, all with several aggressive flushes, 100% take on 10-20 jars each time, 100% without contamination, using B+ and GT spore syringes from Shroomery trusted vendors. Ive spent what must be hundreds of hours sifting through the threads here and truly appreciate the education, as well as the lessons my mushrooms teach me.
Now I had 4 fully colonized agar plates, made myself with teks found here, of which I used one to make a few LC jars, and one to try wedge transfer to brf cakes and to make an LI syringe using teks I found here that seemed well-liked. Unfortunately, my LC failed again (3 jars with the agar wedges, 1 with a fresh cut from inside a fresh picked mushroom) I wish I could get a viable non-contaminated LC but idk what i'm doing wrong...2 of the jars were ELME only, 2 were ELME/dextrose, both at 4% total:water ratio... all 4 jars looked too dark despite coffee filtering twice then PC'ing again, and had barely any growth around the agar wedges/shroom in the LC jars, and when I tried using them to inoculate cakes had no growth at all in 3 weeks...only let them sit that long bc I have enough fully colonized brf cakes and flushes going in my 2 SGFC's that my hands are full enough atm.
The LI i made with the myc from the agar also didn't take after immediately injecting to sterile brf cakes.

So I still have 2 fully colonized agar plates from my more aggressive growing mushrooms, and 4 more plates I am watching expand (beautiful rhizomorphs growth) now which I made using small wedge transfers from the other 2 plates. I have a couple bags I bought from outgrow, which I wanna try inoculating: a manure bag and a rye bag (decided to try first time with these items from a pre-sterilized, pre packaged source, though I make my brf cakes on my own)
Have 1 jar of rye I made and sterilized in the PC a week ago...only reason not inoculated is bc I didn't wanna inoc that with spore syringes, I wanna use live myc. I heard properly prepped rye can sit without inoculation for a while? And I have a ton of 1/2 pint jars I can make more brf cakes in.
My next step is to inoculate something, anything, with live mycelium from my agar plates.
So far I have not been successful with making a viable LC, LI, or even wedge transfer to brf (yes without the dry verm layer, tyvek filter in lid instead)
The wedges transferred to new agar plates are growing great. No signs of the white bacteria I hear people mistake for mycelium...just perfect rhizomorphs growth similar to the initial growth in my brf cakes. So I am convinced my agar plates are good. Shroomery is full of agar making/pouring teks, but disproportionately low on what people do with their agar once its there, grown, stored in the fridge, waiting for use..

Also, I have access to basic medical supplies like hospital grade sterile water, sterile syringes/needles. I have my PC, I have a SAB, plenty of 70% iso alc. . .


TLDR:

I have agar plates growing, (lets give the benefit of the doubt to my babies), uncontaminated mycelium, 1 even pinning in the petri dish, IN the fridge which is very cold!

My questions are:
1) What should I do with them to inoculate brf cakes (1/2 pint PF tek jars)?
2) What should I do with them to create a viable LC
3) Does anybody prefer making their LC's using fresh mushrooms, because I have more of those popping up all the time right now
4) Do any of you make LC's or LI's using shredded fully colonized/consolidated brf cakes?
5) On the side, I want to get the rye and manure bags I bought started. They are presealed, sterile, with injection ports and air exchange ports. What is best to inoculate them with given what I have available (agar, colonized brf cakes, fresh shrooms)
6) If I have to make one of those DIY jar-with-blender-blade things that can be sterilized in the PC, what is the simplest one to make involving the least $ and bob-the-builder skillz? lol

Thank you all, so very much

- "Out of my mind. Back in 5 minutes!"
- "Don't take life too seriously. You'll never get out of it alive."-Edward Hubbard
- "My haters are my biggest motivators."


Extras: Filter Print Post Top
Re: Got agar, whats next? [Re: stbernard]
    #25696158 -

I Don’t mess w cakes.  Just put the agar in rye jars, then g2g if you still want more.  Bags are annoying but that’s my opinion for what it’s worth.


--------------------
Need a lift?

Extras: Filter Print Post Top
Re: Got agar, whats next? [Re: BeefExtreme]
    #25696421 -

Thank you!

Extras: Filter Print Post Top
Re: Got agar, whats next? [Re: stbernard]
    #25696429 -

Agar to grain then g2g. I'm in the middle of just that right now.

Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Sporeworks.EU Spores for European Microscopy   North Spore Bulk Substrate   Original Sensible Seeds Bulk Cannabis Seeds   MagicBag.co All-In-One Bags That Don't Suck   Myyco.com Golden Teacher Liquid Culture For Sale   Mushroom-Hut Grow Bags

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Similar ThreadsPosterViewsRepliesLast post
* What to do with agar? HerbanShaman 1,927 15 04/21/05 02:27 AM
by MushroomFriend
* ...AGAR Help!!!Sterilizing poo....
( 1 2 all )		 FungusMan 3,845 21 01/31/06 07:02 AM
by RogerRabbit
* Using agar to inoculate liquid culture. sexymoreno 1,365 2 08/26/05 03:57 AM
by sexymoreno
* rhizomorphic growth ? KainMagus 2,858 6 07/09/05 02:51 PM
by LeftyBurnz
* rhizomorphic isolate going back to linear / tomentose ohmatic 4,963 15 01/19/05 01:47 PM
by tripndicular
* WBS... Agar method... kimikiri 2,411 9 07/21/05 08:10 AM
by kimikiri
* buying agar - help sinisha 3,821 6 03/31/06 05:49 AM
by FractalXplora
* cheapest glass petri dishes, agar, dextrose, etc Fluxburn 8,342 8 02/14/05 07:57 AM
by Roadkill

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, the_chosen_one, LogicaL Chaos, 13shrooms, hamloaf, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
275 topic views. 12 members, 1,731 guests and 61 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2026 Mind Media. Some rights reserved.

Generated in 0.027 seconds spending 0.008 seconds on 14 queries.