Home | Community | Message Board
You are not signed in.
Sign In New Account
Forum Index Search Posts Trusted Vendors Highlights Galleries FAQ User List Chat Store Random Growery


This site includes paid links. Please support our sponsors.

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: North Spore Bulk Substrate   Sporeworks.EU Spores for European Microscopy   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Substrate Bags   MagicBag.co All-In-One Bags That Don't Suck   Myyco.com Golden Teacher Liquid Culture For Sale

Jump to first unread post Pages: 1 | 2 | 3 |  [ show all ]
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Licky711 first grow the journey begins
    #25635313 -

So last night I made a sab for the first timeout of a 100 liter plastic container. I pulled my Petri dishes from the fridge they were premade lab quality potato dextrose agar vacuum sealed so didn’t autoclave them. Sanitized the sab with Lysol and rubbing alcohol put my gloves on pulled my Golden Teacher spore print out scraped it with the inoculation loop and sneed it over two Petri dishes pulled the Parafilm out and wrapped them the best I could and put them on the top shelf. The temp is about 25-27 Celsius humidity is kinda low only 35-40% I have to run a space heater to keep the room warm.

So a few questions 1. how long should it take for me to see in there is in contamination?
2. What temperature should I keep it at?
3. How’s the humidity ? And what should it be at?
Thanks everyone for all your knowledge and help .

Extras: Filter Print Post Top
Re: Licky711 first grow the journey begins [Re: Licky711]
    #25635434 -

I’m going to do 2 b+ , 2 Texas/PE6, 2 treasure coast, Panaeolus Cyanescens Jamaica, 2 Brazilian cubs. Today today is a good day. Also I just ordered a humidifier and I’m looking for a flow hood to order since the last manufacturer wasn’t able to send it.


--------------------
When your mind looks into itself, dharmata will dawn from within.
-Guru Rinpoche

Extras: Filter Print Post Top
Re: Licky711 first grow the journey begins [Re: Licky711]
    #25635488 -

1. Soon
2. Comfortable for you
3. Doesn't matter for agar


--------------------
"How it should & shouldn't look "      "Recognizing contamination"      "Rye Berry Prep"      "Franks G2G"      "Humidity Chamber"      "Agar Recipes"

"How to dial in your monotubs like a Boss"      "Pastywhyte's Easy Agar Tek"      "Elementary [Damion5050]Coir Tek"        "SGFC"      "SAB Tek"      "Easy AF Shoeboxes"


Extras: Filter Print Post Top
Re: Licky711 first grow the journey begins [Re: Shroomspective]
    #25637551 -

Shroomspective said:
1. Soon
2. Comfortable for you
3. Doesn't matter for agar


Thanks Shroomspective.


--------------------
When your mind looks into itself, dharmata will dawn from within.
-Guru Rinpoche

Extras: Filter Print Post Top
Re: Licky711 first grow the journey begins [Re: Licky711]
    #25637552 -

So I just picked up a uv disinfectant light for the grow room and a humidifier. Does anyone have experience with using a uv light in there mushroom room any tips or tricks to keep a clean environment?


--------------------
When your mind looks into itself, dharmata will dawn from within.
-Guru Rinpoche

Extras: Filter Print Post Top
Re: Licky711 first grow the journey begins [Re: Licky711]
    #25637597 -

Honestly, they're both unneeded. A germicidal lamp only works in a proper clean room where the surfaces are unable to harbour any contaminants. Any carpet or furniture in the room can harbour contaminants and shield them from the light. A germicidal lamp will only create harmful concentrations of ozone in the room you'll be working in.

More importantly: as long as you practice proper sterile technique in a SAB, you could be working in an ebola-infested crack den and still have good results.

The humidity in the room doesn't matter. If you're going to do a monotub (it sounds like you are), then the humidity in the tub itself isn't even that important. Just keep the substrate glistening so that water continually evaporates from the surface.

Extras: Filter Print Post Top
Re: Licky711 first grow the journey begins [Re: Licky711]
    #25637750 -

SadTurkey said:
Honestly, they're both unneeded. A germicidal lamp only works in a proper clean room where the surfaces are unable to harbour any contaminants. Any carpet or furniture in the room can harbour contaminants and shield them from the light. A germicidal lamp will only create harmful concentrations of ozone in the room you'll be working in.

More importantly: as long as you practice proper sterile technique in a SAB, you could be working in an ebola-infested crack den and still have good results.

The humidity in the room doesn't matter. If you're going to do a monotub (it sounds like you are), then the humidity in the tub itself isn't even that important. Just keep the substrate glistening so that water continually evaporates from the surface.


:whathesaid:


--------------------
Lets keep this sacred medicine alive and well! I embrace the shamans, psychonauts, and alchemists of our time :mushroom2:

Extras: Filter Print Post Top
Re: Licky711 first grow the journey begins [Re: BloomingAlchemy]
    #25639392 -

My agar hasn’t grown anything in three day. What should I do? How long does it take to see growth?


--------------------
When your mind looks into itself, dharmata will dawn from within.
-Guru Rinpoche

Extras: Filter Print Post Top
Re: Licky711 first grow the journey begins [Re: Licky711]
    #25639476 -

Depends how old the print is/how dry the spores are. Could be anything from a couple of days to a couple of weeks:shrug:


--------------------


"great things may come to those who wait, but only what's left by those who hustle"

Extras: Filter Print Post Top
Re: Licky711 first grow the journey begins [Re: Bumholio]
    #25639525 -

Bumholio said:
Depends how old the print is/how dry the spores are. Could be anything from a couple of days to a couple of weeks:shrug:


They were pretty fresh spores from a sponsor from this site. The spores were almost wet lol


--------------------
When your mind looks into itself, dharmata will dawn from within.
-Guru Rinpoche

Extras: Filter Print Post Top
Re: Licky711 first grow the journey begins [Re: Licky711]
    #25639577 -

Give it 14 days for stubborn spores.


--------------------
🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿

Extras: Filter Print Post Top
Re: Licky711 first grow the journey begins [Re: JHOVA]
    #25639906 -

JHOVA said:
Give it 14 days for stubborn spores.


Thanks dude I used potato dextrose agar is it good for cubes? Sorry English is not my first language.


--------------------
When your mind looks into itself, dharmata will dawn from within.
-Guru Rinpoche

Extras: Filter Print Post Top
Re: Licky711 first grow the journey begins [Re: Licky711] * 1
    #25640174 -

Potato dextrose agar is fine. Did you follow a specific recipe?

Extras: Filter Print Post Top
Re: Licky711 first grow the journey begins [Re: SadTurkey]
    #25641188 -

SadTurkey said:
Potato dextrose agar is fine. Did you follow a specific recipe?


Sadturkey it was premade agar dishes I bought from a lab. I still haven’t made my own dishes.


--------------------
When your mind looks into itself, dharmata will dawn from within.
-Guru Rinpoche

Extras: Filter Print Post Top
Re: Licky711 first grow the journey begins [Re: Licky711]
    #25641273 -

Licky711 said:
So I just picked up a uv disinfectant light for the grow room and a humidifier. Does anyone have experience with using a uv light in there mushroom room any tips or tricks to keep a clean environment?


Slow down and take a deep breath. You don't need to run out and buy fancy gadgets.  You don't need anything other than some of the simple methods listed at the top of the cultivation forum and good sterile technique.  It's very exciting in the beginning but focus on the basics first.  Learn how to make your own agar dishes, get your sab techniques down, learn how to do a simple transfer with your plates. That will serve you better than over complicating things.  Good luck and be patient - these things take time.

Extras: Filter Print Post Top
Re: Licky711 first grow the journey begins [Re: Jesh]
    #25641545 -

you might want to make you own agar. you can buy agar-agar powder and then go to a home brewery store/beer store and ask for "light malt extract". LME is crystal clear. the reason you want LME is so you can make agar weaker or stronger depending on what you need for your culture.

but since you already have premade just use that and if its too strong dilute it with more water. when i say strong i mean that the growth on the plate has a tendency to grow very dense and "tomentose". when you use "weak" agar concentration you get ropey "rhizomorphic" growth that is easy to distinguish from contaminants that hitch a ride.


--------------------
🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿

Extras: Filter Print Post Top
Re: Licky711 first grow the journey begins [Re: JHOVA]
    #25648302 -

So it’s been 8 days only see some light small growth. and on one plate a small green or brown satellite colony I’ll add pictures in the morning


--------------------
When your mind looks into itself, dharmata will dawn from within.
-Guru Rinpoche

Extras: Filter Print Post Top
Re: Licky711 first grow the journey begins [Re: Licky711]
    #25649721 -

I don’t know what to think about these agar plates is it doing ok ? It’s been 8 days and they haven’t grown much? What should I do now ? It’s my first time doing any of this.


http://







--------------------
When your mind looks into itself, dharmata will dawn from within.
-Guru Rinpoche

Extras: Filter Print Post Top
Re: Licky711 first grow the journey begins [Re: Licky711]
    #25649728 -

That's ok let them grow out a bit then post again in like 4 or 5 days to see where's best to transfer from:thumbup:


--------------------


"great things may come to those who wait, but only what's left by those who hustle"

Extras: Filter Print Post Top
Re: Licky711 first grow the journey begins [Re: Bumholio]
    #25649770 -

Bumholio said:
That's ok let them grow out a bit then post again in like 4 or 5 days to see where's best to transfer from:thumbup:


Ok will do thanks dude

Extras: Filter Print Post Top
Jump to top Pages: 1 | 2 | 3 |  [ show all ]

Shop: North Spore Bulk Substrate   Sporeworks.EU Spores for European Microscopy   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Substrate Bags   MagicBag.co All-In-One Bags That Don't Suck   Myyco.com Golden Teacher Liquid Culture For Sale

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Similar ThreadsPosterViewsRepliesLast post
* *NewTitle* Just help me before i start putting $$ into this shroomizzy 768 9 03/25/03 06:45 PM
by shroomizzy
* Germicidal light bulb
( 1 2 all )		 Land_Crab 4,635 28 10/02/04 08:18 AM
by Land_Crab
* Germicidal UV lights archivist 1,673 19 10/23/17 12:03 PM
by mustangbob3
* EPROM eraser- UV germicidal mogur 2,045 7 04/14/06 03:31 AM
by DrAbominable
* Protection from powerful uv rays.. Ideas anyone? (germicidal bulbs and gamma sterilization) NINJA 780 4 07/11/05 02:55 PM
by NINJA
* Germicidal UV Light Sterilization. Eat The Rich 3,580 14 12/17/14 12:32 PM
by sauroman1
* Germicidal Lamp Enki 1,046 6 10/20/06 01:21 AM
by RogerRabbit
* UV Germicidal Bulbs gsmith 820 4 12/15/04 10:42 AM
by gsmith

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, the_chosen_one, LogicaL Chaos, 13shrooms, hamloaf, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
1,059 topic views. 8 members, 200 guests and 200 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2026 Mind Media. Some rights reserved.

Generated in 0.032 seconds spending 0.01 seconds on 18 queries.