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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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(newbie) agar help?!
    #25528463 -

Hi i was wondering if my agar has been contaminated by mold or if mycelium is starting to grow?


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"The task we face today is to
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Re: (newbie) agar help?! [Re: ExoMyco]
    #25528504 -

Hard to tell, give it a day


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Re: (newbie) agar help?! [Re: Noisecontrol]
    #25528520 -

Thanks for the reply! i will post more pictures in a day or two and hopefully they'll show signes of clear mycelium "root" like structure, and i forgot to add that I've inoculated the agar about 8 days ago.


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"The task we face today is to
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~Paul Stamets~

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Re: (newbie) agar help?! [Re: ExoMyco]
    #25530857 -

From print or syringe?


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Re: (newbie) agar help?! [Re: Noisecontrol]
    #25531166 -

It's hard to tell from the pic, but there is enough mycelium there to do some transfers. Make
some more plates and clean it up some more.


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Cultivating Panaeolus cyanescens


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Re: (newbie) agar help?! [Re: Noisecontrol]
    #25531804 -

started theses plates from a unknown spore print. the only thing that's for sure is that it's a cube strain.


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"The task we face today is to
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~Paul Stamets~

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Re: (newbie) agar help?! [Re: Asura]
    #25531810 -

Thanks for the reply :smile:! I will transfer as soon I get my new petri dishes, I will have them Monday.


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"The task we face today is to
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~Paul Stamets~

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Re: (newbie) agar help?! [Re: ExoMyco]
    #25531872 -

I don't know if I'm paranoid but that looks like mold to me.
Best to use a loop to streak spores so you know where mycelium is and where mold is or just make a dot in the middle at the bottom of a dish with a marker and then drop spores on that dot.


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Nothing breads nothing

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Re: (newbie) agar help?! [Re: Geinstein]
    #25532849 -

Geinstein said:
Best to use a loop to streak spores so you know where mycelium is and where mold is


:whathesaid:

You can make one from wire, plenty of DIY's on here to search up. I bought mine with a culture toolkit. The idea is a z pattern swab motion dragging the spores so you know based on where the colony is originating from. You have to be aware of dirty prints tho because it does happen a lot


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Re: (newbie) agar help?! [Re: Noisecontrol]
    #25532950 -

Yeah I made one with wire and for the handle i used an exacto knife, but like a dumb ass i mixed the spores in steril water then streaked my agard everywhere ... so that's why im a bit nervous. Should of done more research before hand... i guess i got too exited :P.Oh and I've just received my agar plates ill be transferring the "mycelium" in the new plates tonight!

Ill update with pics before the transfer


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"The task we face today is to
understand the language of nature"

~Paul Stamets~

Edited by ExoMyco (10/12/18 11:25 PM)

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Re: (newbie) agar help?! [Re: ExoMyco]
    #25533180 -

Yeah man any water on a plate is askin for trouble.

If you don't mind sharing, what made you decide to go the pre-pour route?


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Re: (newbie) agar help?! [Re: Noisecontrol]
    #25533187 -

Looks like mold to me


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Re: (newbie) agar help?! [Re: Noisecontrol]
    #25533317 -

well to be honest I was following the guidelines in a kit i bought but it turned out the kit was total shit. I bought it at a hightimes in montreal for 175$ (still got the box if yall dont believe me) and the seller refused to show me the inside before buying!!!(for the price i was hoping for a descent kit right?!) it included a cheap book about mushrooms, a  NON STERIL PLASTIC loop, one spore print, enough agar for 500ml, 20 plates and soil like medium, I threw out the medium and loop :\ At that point i knew i fucked up, so i decided to do my own research online and found a lot of good videos (willymyco on vimeo was really good!!) and threads (like here where the community is really kind with newbies :wink:).

Edited by ExoMyco (10/12/18 11:51 PM)

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Re: (newbie) agar help?! [Re: ExoMyco]
    #25533354 -

Pictures before I transfer



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~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~

"The task we face today is to
understand the language of nature"

~Paul Stamets~

Edited by ExoMyco (10/12/18 11:17 PM)

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Re: (newbie) agar help?! [Re: ExoMyco]
    #25533426 -

I found this video on youtube which kinda makes me think of my plates


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"The task we face today is to
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~Paul Stamets~

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Re: (newbie) agar help?! [Re: ExoMyco]
    #25533598 -

That last one looks like contamination for sure. The rest I still wouldn't speak on


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Re: (newbie) agar help?! [Re: Noisecontrol]
    #25542236 -

theses are the transfers 4 days later


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"The task we face today is to
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~Paul Stamets~

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Re: (newbie) agar help?! [Re: ExoMyco]
    #25542370 -

First 2 are blurry, but appear to be creamy looking?  May indicate the presence of bacteria... 3rd looks workable IMO


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Re: (newbie) agar help?! [Re: Caps McGee]
    #25552723 -

thanks for the reply! yeah the camera i had wasnt the best :/ the first two are actually really white and colonized the hole petri dishes after 7days. I then took new transfers from the nicest mycelium dish. theses dishes are 60mm not the 100mm. I'll soon put them in grain with gypsum.

Edited by ExoMyco (10/20/18 01:54 PM)

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