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Re: Cold weather P. cubensis cultivation [Re: mentat]
    #24939428 -

I'd assume 2.5 inches would be well enough for a shoebox.
The size and bad yield were probably due to hidden contaminants + bad surface conditions. I got trich and cobweb-looking gray mold on that first shoebox and tossed it today. It was MS to grains so it's not really strange, probably had plenty of bacteria even if the spawn looked and smelled fine.


Now three out of the remaining 4 shoeboxes are showing hyphal knots. I should have some more fruits for the next week. Let's see if I have more luck with these.

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Re: Cold weather P. cubensis cultivation [Re: Ullr]
    #24954277 -

Now this leaves me baffled. Two of the shoeboxes are pinning.
One spawned the 14th January and the other the 18th. Both have been kept in the same closet, same temperature, same water content, same spawn ratio, same fruiting conditions (reversed lid if I'm not around, since pinning open air and mist as the beads dry if I'm around to take care of it). No signs of contamination that I'm aware of, no metabolites, no bruising, no outreaching mycelium and smells good.


Both have some side pins, not many, there's not really a gap big enough for them to grow. But the later one has hundreds of top pins and primordia (including a few blobs/mutants), while the earlier one has 15 or so pins, and a big one that just went from pin to opening the cap with no veil developing.

As for the differences, the earlier one has almost no top layer (forgot to set aside some CVG before mixing the spawn), I leveled the sub without packing it down, as usually advised.


The later one has a thin top layer and was lightly packed down.

Earlier shoebox with its single shroom:


Later shoebox. Nevermind the crater. Someone accidentally pushed me while taking photos and I shoved my phone into the sub  :facepalm3:


So... help me here, please. Did I do something wrong that can explain this? How are my surface conditions looking like? Crappy genetics or environmental factors?

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Re: Cold weather P. cubensis cultivation [Re: Ullr]
    #24959121 -

Pinset update for the 2nd shoebox. Not really a flush, just some pins randomly popping here and there every once in a while. The caps opened even earlier on these (which has been discussed here), and I already had to pick three dwarf shrooms, plus it's getting plenty of side pins now.




Pinset on the 3rd shoebox is looking far better despite the phone incident, with some interesting pale genetics. It has a single, tiny side pin. I'm seriously puzzled about the difference.


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Re: Cold weather P. cubensis cultivation [Re: Ullr]
    #24968524 -

Finally got some spare time to update this.

Shoebox #2 was tossed after its few pins aborted and it started smelling funky.
Dry yield: 0,5 gram :facepalm3:
No big deal though. Let's not forget these are MS to grains, and probably bacterial to whatever extent.

Shoebox #3 was harvested a couple of days ago. The fruits were thicker and denser than those on box #1, plus light pigmented but, unfortunately, most pins aborted. I took a couple of clones from the bigger fruits. There was one single side pin.
Dry yield: 12 grams, 3 of which were aborts.



Also got some mutants:



Shoebox #4 is pinning really unevenly. First pin appeared days ago, later on a couple more, and today I found 5 or 6 popping up from the surface. No clustering whatsoever, just random isolated pins and a ton of fat side pins.
That first pin is already the biggest shroom I've grown to date, and is showing some interesting PE-like traits. I'm cloning it:



Shoebox #5 is also starting to flush, throwing some slim shrooms. Pinset is still filling in:



...and it's side and bottom pin city:



It looks like this "Z strain" syringe came with an interesting variety of genetic traits.

I also inoculated some grains with the clones via agar.

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