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Offlinehitterg
enthusiast
Registered: 05/16/00
Posts: 179
Last seen: 14 years, 3 months
Re: What are the advantages to this?
    #23989 - 07/12/00 03:25 AM (22 years, 2 months ago)

I think the only disadvantage would be if any of the water evaporated out of the substrate. Just remember, even if the jars come out fine after a few months, you could still get contams when you innoculate the jars, or if the spore print/syringe has a contam in it.

Good Luck!



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Anonymous

Re: What are the advantages to this? [Re: hitterg]
    #23991 - 07/12/00 04:32 AM (22 years, 2 months ago)

Your best bet is to do everything at once. Making up your jars and then letting them sit for weeks is only asking for trouble!

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Anonymous

Re: What are the advantages to this? [Re: hitterg]
    #23992 - 07/12/00 05:05 AM (22 years, 2 months ago)

No water could have evaporated since the jars were air tight. I am aware the jars can get contaminated when innoculating, so I hope I did that right.


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OfflineSuntzu
Geek
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Registered: 10/15/99
Posts: 1,396
Last seen: 3 months, 25 days
Re: What are the advantages to this? [Re: hitterg]
    #23993 - 07/12/00 06:52 AM (22 years, 2 months ago)

One other thing to remember is that even if something comes out of your pressure cooker sterile, that doesn't mean it's contam proof. Cooking grain, anyway, some of the boil-over runs on the sides of jars down into the bottom. The result is a weak little nutrient broth that has coated parts of jars. Sterile, but dangerous as it can act like a wick for anything that might get a foothold on your jar. Especially weeks down the road. The good cap'n is right, do it all asap and beat out any would-be competitors.


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OfflineHAPPYDAYS
enthusiast
Registered: 06/25/00
Posts: 110
Last seen: 21 years, 6 days
Re: What are the advantages to this? [Re: hitterg]
    #23994 - 07/12/00 09:53 PM (22 years, 2 months ago)

perhaps you could resterlize the jars. (maybe)


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Offlineatmos
member
Registered: 06/25/00
Posts: 81
Last seen: 20 years, 8 months
Re: What are the advantages to this? [Re: hitterg]
    #23995 - 07/12/00 10:06 PM (22 years, 2 months ago)

i prepared jars ahead of time once, only a few days....and all of them got contams. pissed me off :frown:

i say do it all at once, make the substrate, jar it, sterilize it, let it sit until cooled, then innoculate the suckers :smile: time consuming, and sometimes frustrating, but oh-so-delicious...........



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OfflinepsilocyberV
old hand

Registered: 06/09/99
Posts: 1,836
Last seen: 10 months, 30 days
Re: What are the advantages to this? [Re: hitterg]
    #23996 - 07/13/00 12:09 AM (22 years, 2 months ago)

Post deleted by administrator.


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Anonymous

Re: What are the advantages to this? [Re: hitterg]
    #23997 - 07/13/00 01:22 AM (22 years, 2 months ago)

As I think I mentioned, the jars were air tight, so evaporation is immpossible. Anyway, the contams aren't in the three jars. My point with doing it this way is that I filtered out the jars that weren't completely sterile. It shouldn't be too much hassle to have a few pre-made jars laying around, you know- make a lot, and then innoculate what you have room for in your terrarium, or how much you want to grow. Then if you have some extra, you can save them. I KNOW the jars I used were contam free, a fact few other people can garuntee after the jars leave the pressure cooker. (I don't even have a pressure cooker, so I had to steam them. I was worried about the effectiveness of this situation, but I do know that three of the jars made it.

Personally, I don't have much use for jars sitting around. When I make a jar, it will probably pay for me to just go ahead and innoculate it. But if I start growing a lot, and growing space becomes an issue, then I think this way may pay off as an additional protection against contams.

I doubt the nutrients in the jar are breaking down, and since the jars are air tight and sterile, they should be just as good in five months as they are right after they cooled to a suitable innoculating temperature.

Anyway, we'll see what happens. I have a digital camera, so hopefully I'll have some nice pics to share with everyone in a month.



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OfflineMuffinBear
liek

Registered: 05/27/00
Posts: 584
Last seen: 12 years, 2 months
Re: What are the advantages to this? [Re: hitterg]
    #23998 - 07/13/00 08:03 AM (22 years, 2 months ago)

Esh, anything thats been sittin around my house for 5 months i dont wanna be messin with. maybe a week at the most i would let them sit. from what i've read 3 days is the optimal? time to let them sit after sterilization just to make sure no contams are growing. but hell, if you use your 2 month old jars and everything works out fine, go with it. you'll never know unless you try.


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