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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Post Your New Tools Of The Trade. [Re: Peleus] * 3
    #29487264 -

Peleus said:
Nokumana said:
Quick update to the SAB today




How is this setup going for you Noku? I've found a local distributor and I'm itching to order.
Also, did you use any silicone or anything else to ensure an airtight seal against the sab walls?


Other than the fact that I dreamed last night that every plate I had inoculated recently got contaminated (🤣🤣🤣), it's actually going really well.

The inspection hatch covers were pretty plug and play. I basically put the bezel on the SAB and traced it with a sharpie and then cut The circles out with a Dremel (wear your safety glasses!). I cut them small and gradually adjusted for a tight fit, so no silicone necessary. The one on the right actually doesn't fit perfectly and I've been meaning to adjust it a little bit. Silicone would work but so would screws. Other than not having a permanent home for my SAB, I've been really happy with it.


--------------------
-Noku
Eskawata kaya waikiki
HHG
"Shake it like you would a newborn baby" - BeefExtreme

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Re: Post Your New Tools Of The Trade. [Re: Nokumana] * 9
    #29489895 -

After much debate with myself, I gave up on building an induction sterilizer and got an infrared one instead.


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Re: Post Your New Tools Of The Trade. [Re: Peleus] * 3
    #29489904 -

Peleus said:
After much debate with myself, I gave up on building an induction sterilizer and got an infrared one instead.




Nice, those seem like a good option.


--------------------
This hobby has taught me many things.
Most of those things are patience.

The Hitchhiker's Guide to the Shroomery
The Official Shroomery Cultivation Museum
Hobbit’s Secondhand Mycology Challenge
Round 2

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Re: Post Your New Tools Of The Trade. [Re: Scemilope]
    #29489939 -

Scemilope said:
Peleus said:
After much debate with myself, I gave up on building an induction sterilizer and got an infrared one instead.




Nice, those seem like a good option.


I agree. I use an induction coil right now but both of these devices evoke the same question in my head.. Why place it outside the SAB?  Constant moving of your arms and hands in and out of the SAB, it seems to me, will only increase the turbulent airflow and kick stuff up that can land on your plates or in your jars. There is no open flame so there shouldn't be an issue with explosion risk. Also, I don't spray the inside of my SAB with ISO (although I do keep a jar of it inside the SAB to drop my scalpel into in between uses)... So what is the downside of keeping the sterilizer in the box versus outside?


--------------------
-Noku
Eskawata kaya waikiki
HHG
"Shake it like you would a newborn baby" - BeefExtreme

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Re: Post Your New Tools Of The Trade. [Re: Nokumana] * 3
    #29489964 -

Nokumana said:
Scemilope said:
Peleus said:
After much debate with myself, I gave up on building an induction sterilizer and got an infrared one instead.




Nice, those seem like a good option.


I agree. I use an induction coil right now but both of these devices evoke the same question in my head.. Why place it outside the SAB?  Constant moving of your arms and hands in and out of the SAB, it seems to me, will only increase the turbulent airflow and kick stuff up that can land on your plates or in your jars. There is no open flame so there shouldn't be an issue with explosion risk. Also, I don't spray the inside of my SAB with ISO (although I do keep a jar of it inside the SAB to drop my scalpel into in between uses)... So what is the downside of keeping the sterilizer in the box versus outside?


Heat source generates way more air currents than slowly moving your hands in and out. Hot air rises.


--------------------
Method for Small Scale Casing Pasteurization

How much really fits in a 00 capsule?

Micro and Macro Mushroom Photography

Can you freeze mycelium?


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Re: Post Your New Tools Of The Trade. [Re: RoscoeReturns] * 4
    #29489979 -

RoscoeReturns said:
Heat source generates way more air currents than slowly moving your hands in and out. Hot air rises.


This ^^

Also the thing itself gets pretty hot, as well as the air near it. This was the biggest and most powerful I could get - 380W (though my UPS said it's drawing more like 400W). It's quoted up to 850C, and while I don't have any means to confirm it, I can attest that it gets really hot. I don't think it'd be possible to work with it in there even if you wanted to.

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Re: Post Your New Tools Of The Trade. [Re: Peleus] * 6
    #29490176 -

Bagger Vance was taken, so his name is dunkey






I put the wider nichrome tape in, I'm going to have to rig something up to shorten it a bit, the ends glow if I try to crank it up to seal over sawdust.


--------------------
Currently hovering between lurking and post&ghost.  Time to lurk and get to work

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Re: Post Your New Tools Of The Trade. [Re: whiski] * 1
    #29490345 -

what are we lookin at here? i’m slow…


--------------------
Peace to you, friend!
The HHG is a guide to getting started.
New User Welcome Wagon
Cake Wars II: Ends 06/30/2026
GROWER’S BEST photo contest: join the fun!
CAKE WARS III - Starts SEPT 1, 2026

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Re: Post Your New Tools Of The Trade. [Re: flapping_bird] * 4
    #29490397 -

flapping_bird said:
what are we lookin at here? i’m slow…


A big ass heat sealer for bags. I had to stare at it a minute. 😂

That thing is really nice whiski!


--------------------
This hobby has taught me many things.
Most of those things are patience.

The Hitchhiker's Guide to the Shroomery
The Official Shroomery Cultivation Museum
Hobbit’s Secondhand Mycology Challenge
Round 2

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Re: Post Your New Tools Of The Trade. [Re: Scemilope] * 2
    #29490830 -

ooo! that’s next level. The elevation adjustment is also cool. That would solve a “sorta” problem that I have had with trying to get a solid bag seal with the one I’ve got, with bags laying on their side…always an uncomfortable operation.


--------------------
Peace to you, friend!
The HHG is a guide to getting started.
New User Welcome Wagon
Cake Wars II: Ends 06/30/2026
GROWER’S BEST photo contest: join the fun!
CAKE WARS III - Starts SEPT 1, 2026

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Re: Post Your New Tools Of The Trade. [Re: flapping_bird]
    #29490932 -

Yeah. That setup looks like it would make for a smooth workflow.


--------------------
This hobby has taught me many things.
Most of those things are patience.

The Hitchhiker's Guide to the Shroomery
The Official Shroomery Cultivation Museum
Hobbit’s Secondhand Mycology Challenge
Round 2

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Re: Post Your New Tools Of The Trade. [Re: Peleus] * 2
    #29490983 -

Gawd Dayum! That infrared sterilizer is SEXY! :eek:

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Re: Post Your New Tools Of The Trade. [Re: Scemilope] * 1
    #29491111 -

Scemilope said:



[/url]


Hey guys,
I picked up something similar on an impulse buy from a UK supplier but they are shitty with their product description and communication and I didn't really think about it when I ordered it.... Is this lid garbage? How do I use it?  The silicone tube has a luer fitting at the end, and I have a cap for it. There is a stopper on the  outer tube. Why is the outer tube there at all, and why is it so long?  I suspect they used a second piece of tubing because securely attaching a luer fitting directly to the plastic lid is fiddly?

I was thinking this could be used without a SAB but this doesn't seem to be the case?

I am now not sure i want to do LC at all since the contamination can be invisible and only become apparent once it is in my grains....




Edited by Elixirel (02/20/26 12:09 PM)

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Re: Post Your New Tools Of The Trade. [Re: Elixirel]
    #29491123 -

Elixirel said:
Scemilope said:



[/url]


Hey guys,
I picked up something similar on an impulse buy from a UK supplier but they are shitty with their product description and communication and I didn't really think about it when I ordered it.... Is this lid garbage? How do I use it?  The silicone tube has a luer fitting at the end, and I have a cap for it. There is a stopper on the  outer tube. Why is the outer tube there at all, and why is it so long?

I was thinking this could be used without a SAB but this doesn't seem to be the case. I could attach a syringe and sterilise it with the syringe attached, but it seeems that the syringe has to be removed in a SAB and can only later be re-used to make further withdrawals only inside a SAB?






Might be usable.
The tube on the inside should reach the bottom of the jar. The tube coming out the top seems a bit longer than necessary to me.
I'd prefer a cap to put on that luer connector between uses.
It seems like that should be functional.


--------------------
This hobby has taught me many things.
Most of those things are patience.

The Hitchhiker's Guide to the Shroomery
The Official Shroomery Cultivation Museum
Hobbit’s Secondhand Mycology Challenge
Round 2

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Re: Post Your New Tools Of The Trade. [Re: Scemilope] * 2
    #29491127 -

Not sure about that GE filter.

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Re: Post Your New Tools Of The Trade. [Re: Scemilope] * 1
    #29491128 -

Scemilope said:
Might be usable.
The tube on the inside should reach the bottom of the jar. The tube coming out the top seems a bit longer than necessary to me.
I'd prefer a cap to put on that luer connector between uses.
It seems like that should be functional.


Thanks. I guess it does need be used inside a SAB, as contamination could enter when attaching the syringe, right?

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Re: Post Your New Tools Of The Trade. [Re: Elixirel] * 2
    #29491130 -

Disclaimer: I don't use anything like this. But as for sterility, you don't need to leave a syringe attached, you sterilize with the cap on. When you're ready to draw out LC, the bit the cap covers is still sterile. When you sterilize a syringe, you can also cap it, or just keep it in the autoclave bag or jar or whatever you sterilized it in until it's time to use it. Then the sterile end of the syringe is attached to the sterile end of the tubing and you can safely draw out the LC.

As long as you inoculate the LC with clean agar and your technique is good, LC is pretty great. Using a much lower nutrient ratio than you have probably read about (like 0.2% malt to water) really helps keep it super clear, so that any bacteria will be really obvious with its turbidity (cloudiness). Mold is harder to detect in LC, but usually pretty easy to spot on a prospective donor plate.


--------------------
LAGM 2026 grow log | My library/trade list

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Re: Post Your New Tools Of The Trade. [Re: Phaethon]
    #29491138 -

Thank you. Yeah, brain fart about sterilising with syringe atatched. scratch that, lol.  I bought this because I had trouble getting mycelium out using a needle in the past and thinking I can use larger jars with this, but I guess larger jars aren't really necessary.

Edited by Elixirel (02/20/26 12:27 PM)

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Re: Post Your New Tools Of The Trade. [Re: Elixirel]
    #29491139 -

ModularMind said:
Not sure about that GE filter.


Looks like it keeps out house flies, but not anything much smaller.

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Re: Post Your New Tools Of The Trade. [Re: ModularMind] * 2
    #29491143 -

Seems to be some kind of Tyvek?

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Shop: Sporeworks.EU Spores for European Microscopy   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Mushroom-Hut Grow Bags   Myyco.com Isolated Cubensis Liquid Culture For Sale   North Spore Injection Grain Bag

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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