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Offlineresin
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How do you make a slide?
    #1835530 - 08/21/03 12:03 AM (13 years, 3 months ago)

I have acess to a microscope that has a 25x, 65x, and 120xoil lens on it. How can I prepare a slide (with syringes, or a print), and what lens should I use? I also have acess to a digital microscope so I can take pictures. Would 2 different strains of cubensis look different under a microscope?


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Anonymous

Re: How do you make a slide? [Re: resin]
    #1835628 - 08/21/03 12:37 AM (13 years, 3 months ago)

step 1: take a slide plate and put some of the spore filled water on it
step 2: put a cover over it
step 3: look at it under the microscope

didnt you ever take biology?

edit: btw, dont mean to sound like a prick, i just realized it might sound like it


Edited by Anonymous (08/21/03 12:38 AM)


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Offlineresin
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Re: How 2 make a spore slide? [Re: ]
    #1838315 - 08/21/03 08:06 PM (13 years, 3 months ago)

How dark does the syringe have to be, to see the spores? What lens should I use? I never took biology.


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Offlinedomite
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Re: How 2 make a spore slide? [Re: resin]
    #1838373 - 08/21/03 08:25 PM (13 years, 3 months ago)

try all of the lenses.

they will give you different views.


the most important thing will be foucusing the image, as only a very small amount of the projetion will give you a clear image. Start with the micrascope almost touching the slide, and then zoom backwards, slowly, until you see a clear image.


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Anonymous

Re: How 2 make a spore slide? [Re: domite]
    #1839385 - 08/22/03 02:34 AM (13 years, 3 months ago)

shrug, you should be able to tell if there are spores in the slide. you dont need to many or you might not be able to see them clearly. In my highschool we had to take biology, i just thought it was a required subject. sorry for the assumption.


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Offlineresin
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Re: How 2 make a spore slide? [Re: ]
    #1841377 - 08/22/03 06:30 PM (13 years, 3 months ago)

Your cool man, no apology is needed. I think I will try to get a clump onto the slide so I can se it well. Around what size are cubensis spores? Where can I find images of cubensis spores under various lenses?


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Anonymous

Re: How 2 make a spore slide? [Re: resin]
    #1841599 - 08/22/03 07:47 PM (13 years, 3 months ago)

ive seen pics somewhere, i'll try to find them and get back to you


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Anonymous

Re: How 2 make a spore slide? [Re: ]
    #1842483 - 08/23/03 12:49 AM (13 years, 3 months ago)

Micron Magic has microscopic pics of all their spores with a scale. Hope thats what you're lookin for.


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Invisiblemjshroomer
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Re: How do you make a slide? [Re: resin]
    #1843840 - 08/23/03 06:59 PM (13 years, 3 months ago)

Well my owbn site is protected byt he shroomery. WOnt let me paste an image here so here ias the URL of an SEM of A Malaysian spore of . cubensis enl;arged 5000 times. THis is from my colleague Prakitsin Sihanonth, I have alsoposted many of my images.

http://mushroomjohn.com/species.htm

mj


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OfflineSev
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Re: How 2 make a spore slide? [Re: resin]
    #1844123 - 08/23/03 10:05 PM (13 years, 3 months ago)

Quote:

resin said:
How dark does the syringe have to be, to see the spores? What lens should I use? I never took biology. 




Start with the lowest-power lens and move up.  With the oil lens you should be able to resolve bacteria.  :smile:

Be -very careful- when using the oil immersion lens.  It's very easy to crack the slide or the lens if you aren't.  Technically, you should also be using a drop of oil between the lens and the cover slip, but this isn't strictly necessary and, honestly, I don't recommend it unless you have the right kind of oil (balsam oil, IIRC) and lens wipes to properly clean the lens with afterwards. 


--------------------
"Do we want the stars? We can have them. Can we borrow cups of fire from the sun? We can and must and light the world." --"On the Shoulders of Giants", Ray Bradbury

All of my posts are full of fiction and blatant lies.


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Offlineresin
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Re: How 2 make a spore slide? [Re: Sev]
    #1845915 - 08/24/03 06:39 PM (13 years, 3 months ago)

The oil lens looks like a normal lens, but its the longest out of the 3. My buddy has this paper that he cleans the lens with


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