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Hail all fellow shroomsters!I received my spores from spore bank today. I plan to do the bulk tech, at: http://www.shroomery.org/index/par/23534/sty/print/spl/0/par/23534/ , and the Kare Kono tech. (But with my own changes). Thought I'd throw my plan out for others to view and to give me any feed back on how to better my experiment. But for the most part I'll keep you updated on my processes, and progress.
First I ordered two syringes of the B+ strand. I took two cc's to make 200 cc's of mycelium solution with the following tutorial: NOTE: (this is someone Else's POST!, I'm just borrowing it to explain my procedure)
STEP 1 - week and a half
Kare Kono - 200ml liquid mycelium supplies your going to need: 1 package of small microwavable containers 1 bottle cheap imitation light syrup 1 package cheap plastic wrap 1) pour about 200ml's of water (around 5 ounces) into the container. 2) add a teaspoon or two of light syrup to the water 3) put a piece of plastic wrap over the top of the container, and then put the lid on top of that 4) microwave the container, with the lid slightly cracked for 5 Minutes . when its done nuking, open your microwave and push the lid down so its all sealed in there (care full, its hot) 5) let cool for a few hours, or until it is room temperature again, and then lift up the lid slowly, flame sterilize your syringe, and inject through the plastic wrap 1 - 2cc's of your left over spore solution. 6) toss these guys in your incubator (I'm using a double tub setup), put em in a dark area between 80 and 86 degrees. 7) after a few days you should start to see some myc. growth in the solution. after about a week and a half your container should have mycelium in it. 8.) shake the containers around every few days and let the water sloshing break up the myc. no need for glass chunks. 9.) when your ready to inoculate, just lift up the lid, and poke the needle of your sterile syringe through the plastic wrap and suck up how much ever juice you need. 10.) AFTER the spores have germinated several cc's of peroxide (about 5 or so for that much water) should be added through the inoculation hole. The peroxide prevents con tams and speeds up colonization by adding 02 to the karo solution.
STEP 2 - 18 days
Spawn 1.) A quart of birdseed spawn. Birdseed rinsed clean several times in cold water, sunflower seeds float and are easily poured off from the rest of the BS). 2.) Boil an excess of water first. Reduce heat once boiling. 4.) the Seed is simmered on low for 30 minutes (no more since we will be adding peroxide later and don?t want too much moisture). 5.) The grain is strained, rinsed and dried thoroughly before being loaded into the quart jar, ? full. The lid has a filter disc in it, but poly fill, or even coffee filters or cardboard can be substituted since we will be adding peroxide later. 6) The jar is pressure cooked to sterilize and also destroy peroxide decomposing enzymes. 7.) 5 hours later, When cool, the quart is injected with 12 cc?s peroxide, and shaken. 8.) Place all of the following items into the Glove Box.
A.) spore syringes x2 and 200ml B.) Prepared Birdseed in one-quart mason jars (wide mouth = 90mm). C.) Plastic lids with a 1/2" hole drilled into it. D.) 90mm Filters. E.) Small dish of alcohol with the filters in it. F.) An alcohol soaked paper towel. G.) Plastic container 1 of 3 that will all fit inside a large tub all with lids.
9.) Loosen the pressure out of the grain jars but, do not take the lid off! 10.) take the syringes and heat up the needles to kill contaminants. 11.) I was going to then inoculate about three or four jars with the two syringes as well as the 200ml of mycelium solution from the Kare kono tech. 14.) Now you are going to take one filter out of its alcohol filled dish and lift the lid off of a grain jar while simultaneously replacing it with the filter. Take a plastic lid and tighten it down over the filter and onto the jar of grain. 15.) Wait 30 minutes and take the jars out of the Glove Box and begin turning the grain and the mycelium. 16.) Place this in a converted Poor Boys Incubator. 17.) In about 4-7 days you should be seeing growth in your inoculated grain. What you should do next is break-up and rotate the grain again. Set it back into the Incubator and watch closely for contamination signs. 18.) Colonization is complete in a week with temps around 80 deg F. 19.) Prepare A more birdseed using same instructions above. Peroxide amount is proportional to that listed above. 20.) In the flow hood, Dump both the inoculated quart jar and the new birdseed into the tub and seal the lid. (I have 3 non air-tight tubs.) 21.) I'm going to try to fill each of the clean tubs and then place them into a bigger tub. 22.) There is enough room inside the large tub to put a large aquarium heater, inside a vase. I'm hoping that this will be enough to incubate the 3 tubs inside. I want to see if at 86 degrees the box with recolonize in 11 days.