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Mushrooms, Mycology and Psychedelics > Advanced Mycology
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How does a multispore mycelium work?
    #12288170 -

Hi :smile:

I couldn't find much about this particular question but when one inoculates a substrate with a multispore syringe, how many of the spores then germinate monokaryotically and how many of those which then proce hyphae combine into how many dikaryotic mycelia?

These are not necessarily the questions I would like an answer on, what I wonder about is when a fruiting body is produced, are its genetics identical throught the mushrooms and so does it come from 1 single dikaryotic mycelium or a corporation of many? If it comes from one, then how many different dikaryotic mycelia are typically seen at the surface of a PF cake of colonized substrate? I'm not understanding here how many organisms are working together or if it's one in total? Can it be one in total but having several genotypes and phenotypes at different places?

I'd like it if someone could clarify this for me! :laugh:


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    Re: How does a multispore mycelium work? [Re: Solipsis]
        #12288739 -

    Hi :wink:

    I don't know the exact average percent of sprores which germinate when you inoculate, but the ones that do, usuallycombine to form dikaryotic mycelia, anastomosis (and lucky you or you would get no fruits).  Now sometimes there are incompatible dikaryotic individuals on the some chunk of substrate which will produce genetically different fruits, however, I'm pretty sure that in a PF cake, it's usually one individual.

    Although, since most of the mushrooms people are growing here are basidiomycetes or ascomycetes, when the hyphae combine, the nuclei and hence genetic info don't combine right away in the hyphae.  Now this heterokaryosis is important because it means that even though you could consider the mycelium to be one individual, parts can have different nuclei from mutations or from anastomosis and thus different fruits on the same cake.

    Hope that helped clear it up for ya.  I'm a bacteria guy myself and when I started this hobby I had to pick up some literature to wrap my head around their sexual reproduction.:cheers:


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    Re: How does a multispore mycelium work? [Re: whitelight7]
        #12291837 -

    Thanks so much sir :laugh:

    It's still a bit hazy though, you start off with say 1000 spores (in actuality it will probably a LOT more), now these recombine subsequently until there it one single genetic entity? Although during some recombination instances there are mutations making up for the phenotypical differences? So dikaryotic mycelium can recombine again and again through anastomosis if genetically compatible?

    Meaning that if the mycelia are of different substrains the likelyhood of genetic compatibility is lower so you only get a succesful recombined offspring in something like 1 in 100 times right? And in all the other times there will just be a border between incompatible mycelia so that they stay 2 organisms?


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  • Other items - Supplies etc.


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    Re: How does a multispore mycelium work? [Re: Solipsis]
        #12293633 -

    No, they won't keep combining until one individual is produced, why I'm not sure.  the mutations do make up for some phenotypic/genotypic variation, although there is variation caused by the multiple nuclei remaining in the hyphae as well.

    I'm not sure on the ratio of comparability, but I think the less genetically similar, the more likely they are to undergo anastomosis, not the other way around.  I'd have to go back to some literature.... I think it was in Nature where they tested that.  If you have access to journals (if your in college especially), you should look into it.


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    Mushrooms, Mycology and Psychedelics > Advanced Mycology
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