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A new B cell stimulator - GLIS from Ganoderma lucidum
Writer: Jingsong Zhang / Date :2002-09-18 / hits: 32 Jingsong Zhang1,2, Qingjiu Tang1,2, Martin Zimmerman-Kordmann1, Werner Reutter1 and Hua Fan1 1Institut fur Molekularbiologie und Biochemie, Freie Universitat Berlin, Gemany. 2The edible fungi institute, Shanghai Academy of Agricultural Science, China.
Abstract: A bioactive fraction (GLIS) was isolated from the fruiting body of the fungus Ganoderma lucidum using successive chromatographic steps. GLIS is a proteoglycan and has a carbohydrate : protein ratio of 11.5 : 1. The carbohydrate portion is composed of seven different monosaccharides, predominantly D-galactose, D-glucose and D-mannose in the molar ratio of 1 : 3.8 : 1. GLIS stimulated the proliferation of mouse spleen lymphocytes, resulting in a three to four-fold increase in the percentage of B cells. GLIS also activated mouse spleen lymphocytes, and most of the activated cells were B cells. The B cells were enlarged, expressed CD71 and CD25 on the cell surface, and showed a significant increase in the secretion of immunoglobulin. Lymphocytes also showed a slightly increased production of IL-2, whereas the secretion of IL-4 was not influenced by GLIS. Furthermore, GLIS did not influence the intracellular Ca2+ concentration of lymphocytes, but it enhanced the expression of protein kinase C and protein kinase C in B cells. According to our results GLIS is a new B cell-stimulating factor.
Keywords: B cell stimulator, GLIS, Ganoderma lucidum
* This paper is presented at 'The 2nd Meeting of Far East Asia for Collaboration on Edible Fungi Research'.