|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
johnny_poke
Brownian movement


Registered: 12/03/08
Posts: 442
Loc: Europe
Last seen: 10 years, 5 months
|
Parafilm: What am I doing wrong?
#9914722 - 03/05/09 01:41 AM (14 years, 11 months ago) |
|
|
I take the 1 inch wide roll of parafilm and wrap it round the edge of the dish rather tightly, stretching it out as I go, but being careful not to stretch too much, just enough. Everything seems to be fine. I check all the way round and it's nicely sealed. Then, 4 or 5 days later when I check on the progress I notice that there are several splits in the film, allowing unfiltered air to pass into the dishes!
Why is my parafilm tearing like this? Am I wrapping them too tightly perhaps? Maybe my petris are some second-rate bullshit with sharp edges? Or maybe it's just some old-ass parafilm that's seen better days. The country where I live is full of fucking pirates! Even those bastards at the school science supply store.
Please tell me it's something I'm doing wrong. And please tell me this has happened to you too.
Thanks for listening to me whine.
-------------------- [quote]But what our scientist does not realize is that every time he makes a measurement, the Flying Spaghetti Monster is there changing the results with His Noodly Appendage.[/quote]
|
dead
grateful


Registered: 08/28/08
Posts: 2,761
Loc: North pole
Last seen: 9 years, 2 months
|
Re: Parafilm: What am I doing wrong? [Re: johnny_poke]
#9914933 - 03/05/09 04:21 AM (14 years, 11 months ago) |
|
|
Try using micropore tape instead of parafilm.
-------------------- "The third eye. You spend years doing everything you can to open it and then the damn thing opens and your friends laugh at you when you tell them you can see their souls behind their eyes burning like rainbows." Links:
Nibin's Guide for Noobs
some easy teks on bulk & grain prep. (my journal)
|
johnny_poke
Brownian movement


Registered: 12/03/08
Posts: 442
Loc: Europe
Last seen: 10 years, 5 months
|
Re: Parafilm: What am I doing wrong? [Re: dead]
#9914983 - 03/05/09 05:03 AM (14 years, 11 months ago) |
|
|
Quote:
dead said: Try using micropore tape instead of parafilm.
Is that what you do? I paid good money for that 250ft roll of effing parafilm. I'm thinking that the petris have sharp edges. Why the hell would anyone manufacture petris with sharp edges which cannot be wrapped? I don't know. This world is full of bollox.
-------------------- [quote]But what our scientist does not realize is that every time he makes a measurement, the Flying Spaghetti Monster is there changing the results with His Noodly Appendage.[/quote]
|
johnny_poke
Brownian movement


Registered: 12/03/08
Posts: 442
Loc: Europe
Last seen: 10 years, 5 months
|
Re: Parafilm: What am I doing wrong? [Re: johnny_poke]
#9914989 - 03/05/09 05:07 AM (14 years, 11 months ago) |
|
|
-------------------- [quote]But what our scientist does not realize is that every time he makes a measurement, the Flying Spaghetti Monster is there changing the results with His Noodly Appendage.[/quote]
|
veda_sticks
Cultivator




Registered: 07/29/07
Posts: 14,191
Loc: UK
Last seen: 4 years, 1 month
|
Re: Parafilm: What am I doing wrong? [Re: johnny_poke]
#9915129 - 03/05/09 06:46 AM (14 years, 11 months ago) |
|
|
JHohny, please dont post stuff like that here.
you want to post music videos, post it in the music forum.
OP, mayhbe your stretching it too much. Ive never used parafilm or petri dishes so IDK, but thats my thinking is the film has been stretched too much
-------------------- PF TEK - writeup by EvilMushroom666 Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD Cakes can and will pin! - So you think cakes suck for pins. Your wrong Franks Simple Coir/Verm Tek Franks Proper Pasturisation Tek Franks Spawning To Bulk - Monotub Professor Pinheads RTV Injection Port Tek Foo Mans No Soak WBS Prep Tek
|
johnny_poke
Brownian movement


Registered: 12/03/08
Posts: 442
Loc: Europe
Last seen: 10 years, 5 months
|
Re: Parafilm: What am I doing wrong? [Re: veda_sticks]
#9915144 - 03/05/09 06:53 AM (14 years, 11 months ago) |
|
|
Lol, Veda, I am the OP. Yes I suppose I need to refine my technique somewhat. But I assure you that I take the utmost care when doing the wrapping. Has anyone else ever run into this problem?
Oh, and sorry for posting the link, but the song is "Piece Of Crap" by Neil Young and it seemed very pertinent.
-------------------- [quote]But what our scientist does not realize is that every time he makes a measurement, the Flying Spaghetti Monster is there changing the results with His Noodly Appendage.[/quote]
|
veda_sticks
Cultivator




Registered: 07/29/07
Posts: 14,191
Loc: UK
Last seen: 4 years, 1 month
|
Re: Parafilm: What am I doing wrong? [Re: johnny_poke]
#9915151 - 03/05/09 06:56 AM (14 years, 11 months ago) |
|
|
Quote:
johnny_poke said: Lol, Veda, I am the OP. Yes I suppose I need to refine my technique somewhat. But I assure you that I take the utmost care when doing the wrapping. Has anyone else ever run into this problem?
Oh, and sorry for posting the link, but the song is "Piece Of Crap" by Neil Young and it seemed very pertinent.
lol im not awake yet.
odd problem.
-------------------- PF TEK - writeup by EvilMushroom666 Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD Cakes can and will pin! - So you think cakes suck for pins. Your wrong Franks Simple Coir/Verm Tek Franks Proper Pasturisation Tek Franks Spawning To Bulk - Monotub Professor Pinheads RTV Injection Port Tek Foo Mans No Soak WBS Prep Tek
|
durban_poison
myco contractor

Registered: 09/19/01
Posts: 2,417
|
Re: Parafilm: What am I doing wrong? [Re: johnny_poke]
#9915303 - 03/05/09 07:57 AM (14 years, 11 months ago) |
|
|
that sounds like really old parafilm. i have some from 6 years ago and it still doesn't crack. in fact i dont think i have ever seen it crack. could it be your pulling it to tight? it does stretch a long way but only so far.
|
johnny_poke
Brownian movement


Registered: 12/03/08
Posts: 442
Loc: Europe
Last seen: 10 years, 5 months
|
Re: Parafilm: What am I doing wrong? [Re: durban_poison]
#9915346 - 03/05/09 08:17 AM (14 years, 11 months ago) |
|
|
I'm thinking if I wrap it round several times it should remedy the problem. How many times do you think I'd be able to wrap it round without seriously impairing GE?
-------------------- [quote]But what our scientist does not realize is that every time he makes a measurement, the Flying Spaghetti Monster is there changing the results with His Noodly Appendage.[/quote]
|
RogerRabbit
Bans for Pleasure



Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 21 days
|
Re: Parafilm: What am I doing wrong? [Re: johnny_poke]
#9915360 - 03/05/09 08:25 AM (14 years, 11 months ago) |
|
|
It's not fresh. I've had old rolls crack like that before. The other thing is not to get alcohol on the parafilm or wrap while the dish is wet with alcohol. As an added precaution, I keep dishes in ziplock bags, which I inflate with the laminar flow hood. This gives an added layer of protection. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
|
RedDevil420
Antichrist

Registered: 01/03/09
Posts: 1,079
Last seen: 10 years, 6 months
|
Re: Parafilm: What am I doing wrong? [Re: RogerRabbit]
#9915386 - 03/05/09 08:31 AM (14 years, 11 months ago) |
|
|
Get glad cling wrap, shit works. The plastic bag RR mentioned is also a good idea, I use plastic bags continuously for my grow - they're useful.
-------------------- I'm Just Making This All Up. Grains In Glass Jars With Professional Filters FTW. Coir Monotubs FTW. Sterile Technique FTW. Fan dry, Desiccant storage FTW. Ralphster's Spores FTW. Mushroom Cloud - Dirtball
|
johnny_poke
Brownian movement


Registered: 12/03/08
Posts: 442
Loc: Europe
Last seen: 10 years, 5 months
|
Re: Parafilm: What am I doing wrong? [Re: RogerRabbit]
#9915499 - 03/05/09 09:09 AM (14 years, 11 months ago) |
|
|
Quote:
RogerRabbit said: It's not fresh. I've had old rolls crack like that before. The other thing is not to get alcohol on the parafilm or wrap while the dish is wet with alcohol. As an added precaution, I keep dishes in ziplock bags, which I inflate with the laminar flow hood. This gives an added layer of protection. RR
Thanks RR, I was beginning to think that I'm totally incapable or something. Since using glad wrap seems to be acceptable I suppose a few extra wraps of the parafilm can't hurt. No flowhood as yet I'm afraid, and not likely to be able to afford one any time soon either. I will just keep truckin' on with my GB.
Oh, and thanks for the ziplock bag idea, I will do that 
EDIT: And just since you mentioned it... I don't use any alcohol in my sterile procedures, only bleach. I have heard from many sources that alcohol doesn't sterilise, so I can see no point in using it. Can anyone put me in my place on this one?
-------------------- [quote]But what our scientist does not realize is that every time he makes a measurement, the Flying Spaghetti Monster is there changing the results with His Noodly Appendage.[/quote]
Edited by johnny_poke (03/05/09 09:12 AM)
|
Roggen_Rocker
Strangler



Registered: 01/30/09
Posts: 143
Loc: Europe
|
Re: Parafilm: What am I doing wrong? [Re: johnny_poke]
#9915541 - 03/05/09 09:24 AM (14 years, 11 months ago) |
|
|
Quote:
johnny_poke said:
EDIT: And just since you mentioned it... I don't use any alcohol in my sterile procedures, only bleach. I have heard from many sources that alcohol doesn't sterilise, so I can see no point in using it. Can anyone put me in my place on this one?
well I think many people use alcohol for disinfecting their workingspace or items. I use a mix of cutasept (a skin disinfectant which is fungicidal) and rubbing alcohol. Worked fine for me so far.
But that's just my preference since I don't like wearing rubber gloves and bleach wouldn't be good for my precious pianist hands
|
RedDevil420
Antichrist


Registered: 01/03/09
Posts: 1,079
Last seen: 10 years, 6 months
|
Re: Parafilm: What am I doing wrong? [Re: Roggen_Rocker]
#9915896 - 03/05/09 10:52 AM (14 years, 11 months ago) |
|
|
Quote:
Roggen_Rocker said:
Quote:
johnny_poke said:
EDIT: And just since you mentioned it... I don't use any alcohol in my sterile procedures, only bleach. I have heard from many sources that alcohol doesn't sterilise, so I can see no point in using it. Can anyone put me in my place on this one?
well I think many people use alcohol for disinfecting their workingspace or items. I use a mix of cutasept (a skin disinfectant which is fungicidal) and rubbing alcohol. Worked fine for me so far.
But that's just my preference since I don't like wearing rubber gloves and bleach wouldn't be good for my precious pianist hands
Actually alcohol is better than bleach. Others will agree. 70% Isopropyl.
-------------------- I'm Just Making This All Up. Grains In Glass Jars With Professional Filters FTW. Coir Monotubs FTW. Sterile Technique FTW. Fan dry, Desiccant storage FTW. Ralphster's Spores FTW. Mushroom Cloud - Dirtball
|
johnny_poke
Brownian movement


Registered: 12/03/08
Posts: 442
Loc: Europe
Last seen: 10 years, 5 months
|
Re: Parafilm: What am I doing wrong? [Re: RedDevil420]
#9921106 - 03/06/09 02:16 AM (14 years, 11 months ago) |
|
|
Quote:
Roggen_Rocker saidBut that's just my preference since I don't like wearing rubber gloves and bleach wouldn't be good for my precious pianist hands 
Guitarist here The bleach doesn't have any adverse effect on my hands.
RedDevil420, that is not what I've heard, but since I'm no scientist I can't really debate the subject. Bottom line is that bleach does work as I've had no contams as yet. I guess it's each to his own on this one.
-------------------- [quote]But what our scientist does not realize is that every time he makes a measurement, the Flying Spaghetti Monster is there changing the results with His Noodly Appendage.[/quote]
|
RedDevil420
Antichrist


Registered: 01/03/09
Posts: 1,079
Last seen: 10 years, 6 months
|
Re: Parafilm: What am I doing wrong? [Re: johnny_poke]
#9921587 - 03/06/09 08:12 AM (14 years, 11 months ago) |
|
|
Quote:
johnny_poke said:
Quote:
Roggen_Rocker saidBut that's just my preference since I don't like wearing rubber gloves and bleach wouldn't be good for my precious pianist hands 
Guitarist here The bleach doesn't have any adverse effect on my hands.
RedDevil420, that is not what I've heard, but since I'm no scientist I can't really debate the subject. Bottom line is that bleach does work as I've had no contams as yet. I guess it's each to his own on this one.
I think you're right lol.
-------------------- I'm Just Making This All Up. Grains In Glass Jars With Professional Filters FTW. Coir Monotubs FTW. Sterile Technique FTW. Fan dry, Desiccant storage FTW. Ralphster's Spores FTW. Mushroom Cloud - Dirtball
|
RogerRabbit
Bans for Pleasure



Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 21 days
|
Re: Parafilm: What am I doing wrong? [Re: RedDevil420]
#9922015 - 03/06/09 10:18 AM (14 years, 11 months ago) |
|
|
Alcohol is better for sanitizing, but it needs to be 50% to 70% alcohol. Too many growers buy the 91% or 99% alcohol, and it's not nearly as effective, thus the disinformation. Solvents sanitize, not sterilize. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
|
|