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Invisible1stimer
Religion=Rape
Registered: 11/18/01
Posts: 1,280
Loc: Amerika
contam prob?
    #928271 - 10/03/02 12:49 PM (14 years, 4 months ago)

is it safe to say that if you innoculated 5 jars with the same syringe and all the jars were sterilized thoroughly that if there was contamination at all of the innoculation sites on every jar that the syringe was contaminated?


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InvisibleBilge
longtimenoC

Registered: 08/26/02
Posts: 1,858
Loc: USA
Re: contam prob? [Re: 1stimer]
    #928282 - 10/03/02 12:52 PM (14 years, 4 months ago)

other possibilities: the needle wasn't sterilized before you innoc'ed or the needle wasn't sterlized between jars, picked up contams at first jar and spread to the rest.


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Invisible1stimer
Religion=Rape
Registered: 11/18/01
Posts: 1,280
Loc: Amerika
Re: contam prob? [Re: Bilge]
    #928291 - 10/03/02 12:56 PM (14 years, 4 months ago)

the needle was sterilized. if the contamination came from the first jar then shouldn't one of the jars be showing contamination at a different location than the innoculation site? that also means that the contam had to have been picked up on the first innoc. I think that the syringe is contaminated.


--------------------
ash dingy donker mo gollyhopper patty popiton rockstop bueno mayo riggedy jig bobber johnathan pattywhacker gogboob t-shirt monkey.

There is such emotion in the distortion.


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Offlineshroomsformike
Explorer forHuman Life
Registered: 08/13/02
Posts: 178
Loc: somewhere
Last seen: 14 years, 3 months
Re: contam prob? [Re: 1stimer]
    #928331 - 10/03/02 01:17 PM (14 years, 4 months ago)

This is exactly why I use a new in the package sterile syringe for every jar. New tip to colonizee faster. Add a fifth hole in the middle, instead of a resevoir if you plan on dunking . They will assure a 100% colonization always.


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Chief Mojo Rising
,,,where will you be when marshall law is put into effect,,,


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InvisibleBilge
longtimenoC

Registered: 08/26/02
Posts: 1,858
Loc: USA
Re: contam prob? [Re: 1stimer]
    #928342 - 10/03/02 01:21 PM (14 years, 4 months ago)

yep, the syringe could have be contam'ed. just throwing some other ideas out there. i was thinking one of my syringes was contam'ed but only 3 of 6 jars failed making me think that less.


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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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