Home | Community | Message Board


Magic-Mushrooms-Shop.com
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop: ½ Pint Jars, Agar, Laminar Flow Hood

Jump to first unread post. Pages: 1
Offlinefunkyballoon
Pooh-Bah
Registered: 01/19/99
Posts: 311
Last seen: 16 years, 2 months
Re: Does H2O2 kill spores/mycelium?
    #87127 - 08/03/00 10:38 AM (17 years, 3 months ago)

H2o2 destroys spores, but not mycelium. Probably too high a concentration will kill the mycelium, but plain old 3% doesn't offer much of a threat. It's metabolized into its component parts by enzymes.


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinecactus
journeyman
Registered: 05/10/00
Posts: 77
Last seen: 16 years, 3 months
Re: Does H2O2 kill spores/mycelium?
    #87129 - 08/06/00 09:16 PM (17 years, 3 months ago)

funkyballoon:

Let's say I was going to try and make a mycelium-solution syringe by doing the following:

1. cutting off a sugar-cube sized chunk of healthy mycelium from a 100% colonized jar.

2. dipping the chunk of mycelium in regular 3% hydrogen peroxide for a few seconds, and then dipping it in distilled water to rinse.

3. placing the H2O2-rinsed mycelium chunk in a glass jar with a hole in the lid, injecting some distilled water in, swishing around, and then sucking the mycelium-solution back up into a clean syringe.

Would the brief H2O2 bath harm the mycelium in any way? Would it be beneficial in fighting contaminants?

thanks
c



Post Extras: Print Post  Remind Me! Notify Moderator
Offlinecactus
journeyman
Registered: 05/10/00
Posts: 77
Last seen: 16 years, 3 months
Re: Does H2O2 kill spores/mycelium?
    #87130 - 08/06/00 09:25 PM (17 years, 3 months ago)

funkyballoon (again):

Well, sorry to ask a dumb question, I just found the answer in one of your previous posts:
http://www.shroomery.org/ubbnoncgi/Forum2/HTML/012457.html

I guess H2O2 doesn't really hurt mycelium that much...

Another question, though: why do you recommend using the mycelium from a fruit's stem to make syringes, as opposed to mycelium from a 100% colonized cake? Is it because not all of the mycelium in the cake has genetics to fruit, whereas the fruit stem mycelium does?

thanks
c



Post Extras: Print Post  Remind Me! Notify Moderator
Anonymous

Re: Does H2O2 kill spores/mycelium?
    #87131 - 08/07/00 04:19 PM (17 years, 3 months ago)

Another bonus to using the mycelium from the stem is that, unlike colonized substrate, the mycelium in the stem is non-nutritive. When cloning on agar, the flesh from inside the stem or cap is used. With the extra sterile measures of only using the flesh from inside the stem and making the transfer with a glove box, flow hood, oven tek, I can see no reason why this shouldn't work. If you sterilize a half pint jar of water and then add enough 3% h2o2 to equal 5% of the total volume, that should help keep things sterile. If you were to put a blender base on the jar before sterilization, this could help break up the mycelium. You could also add some glass shards to the jar for this purpose.

------------------
"Not all drugs are good... some are great."



Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop: ½ Pint Jars, Agar, Laminar Flow Hood

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

Similar ThreadsPosterViewsRepliesLast post
* Does H2O2 KILL spores or just inhibit germination?
( 1 2 all )
micro 4,610 37 11/26/03 11:24 AM
by debianlinux
* Will Hydrogen Peroxide Kill Spores? Color_of_Sound 6,746 11 05/13/16 11:42 AM
by bodhisatta
* Re: does isopropyl alcohol kill spores? egghead 2,616 8 05/11/01 11:09 PM
by egghead
* Re: Mycelium H2O + H2O2? joee 2,306 6 11/30/99 06:07 PM
by mycofile
* Re: Liquid mycelium with H202 Anonymous 1,119 6 02/11/00 08:31 AM
by Anonymous
* Re: mycelium and H2O2 darkangel932 1,203 5 05/02/01 01:27 AM
by sylo
* clone with h2o2 Anonymous 815 6 01/06/02 12:53 PM
by mycofile
* KILLS TRIC STONE DEAD
( 1 2 all )
SixTango 3,544 21 08/27/03 01:53 AM
by Diploid

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: RogerRabbit, bodhisatta
1,575 topic views. 0 members, 6 guests and 0 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Mycohaus
Please support our sponsors.

Copyright 1997-2017 Mind Media. Some rights reserved.

Generated in 0.025 seconds spending 0.006 seconds on 18 queries.