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 B.I.O.
old hand
 
Registered: 05/26/99
Posts: 458
Last seen: 7 months, 3 days
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 Re: Info on Pan cyan Florida 
#87002 - 07/28/00 01:55 AM (23 years, 2 months ago) |
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hi elektrolurch.....i never had any reports of contaminated pancyan-prints...but i can send you a culture and another print, if you like...BiO.
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Elektrolurch
enthusiast
Registered: 05/01/00
Posts: 307
Loc: Germany
Last seen: 17 years, 6 months
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Re: Info on Pan cyan Florida [Re: B.I.O.]
#87004 - 08/02/00 08:07 AM (23 years, 2 months ago) |
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Hi Bio,thanks, but I'll still try some time. If I shouldn't have success, then I'll be glad to
accept your offer.
BTW I made some photos of the mycelium, but I have to let them be developed, so you can see
what I mean...
What kind of agar do you use Bio? I use standard MEA. Elektrolurch
--------------------
"For all the time spent in that room
The doll's house, darkness, old perfume
And fairy stories held me high on
Clouds of sunlight floating by.", Pink Floyd '67
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B.I.O.
old hand
Registered: 05/26/99
Posts: 458
Last seen: 7 months, 3 days
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Re: Info on Pan cyan Florida [Re: B.I.O.]
#87005 - 08/02/00 04:05 PM (23 years, 2 months ago) |
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we use MYPA....malt/yeast/peptone agar,,,,or we cook up dung and add this to the brew...anyway ....we made clones of the shrooms...so u can have a liquid myc. solution in syringes...if u like... BiO.
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 GGreatOne234
Stranger
Registered: 12/23/99
Posts: 8,946
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Re: Info on Pan cyan Florida [Re: B.I.O.]
#87006 - 08/02/00 04:21 PM (23 years, 2 months ago) |
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i just saw the cope pictures at bio's webpageyou would be amazed at how many variations in appearance this mushroom has when you find them in the wild. the ones in the pictures are all excellent looking copes, must be an isolated strain of copelandias though because they all look identical to eachother. keep on sharoomin!
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Elektrolurch
enthusiast
Registered: 05/01/00
Posts: 307
Loc: Germany
Last seen: 17 years, 6 months
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Re: Info on Pan cyan Florida [Re: B.I.O.]
#87007 - 08/05/00 01:04 PM (23 years, 1 month ago) |
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Hi Bio,thanks, I'll give it a last horse shit try. I sterilized yesterday two jars and I hope it works better. If it shouldn't work...
I've used also MYPA, but to get the peptone is kind of hard for me, last time I had to "steal" it from the lab of my girlfriend in the university. Where do you get it? Merck again? Elektrolurch p.d. T?glich fit, mit zwei gramm shit...
--------------------
"For all the time spent in that room
The doll's house, darkness, old perfume
And fairy stories held me high on
Clouds of sunlight floating by.", Pink Floyd '67
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Anonymous
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Re: Info on Pan cyan Florida [Re: B.I.O.]
#87008 - 08/05/00 03:03 PM (23 years, 1 month ago) |
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------------------
Check out the new site
Quality spore prints & gourmet mushroom cultures
http://www.homestead.com/mycomountain
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Prellgott
addict
Registered: 02/08/00
Posts: 383
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Re: Info on Pan cyan Florida [Re: B.I.O.]
#87009 - 08/09/00 06:57 AM (23 years, 1 month ago) |
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FOAF has 6 jars (BRF/VERM) of pan. Trop ready
and some mycelium syringes, now he wants to sterilize horse shit in the p. cooker..
.....hey Elerolurch...
does it stink (to p. cook jars of shit)?
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i'm back
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Anonymous
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Re: Info on Pan cyan Florida [Re: B.I.O.]
#87010 - 08/11/00 01:29 AM (23 years, 1 month ago) |
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Hey mycomountain, what did you grow those baby's in? dung, dung/compost, compost, straw?
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Elektrolurch
enthusiast
Registered: 05/01/00
Posts: 307
Loc: Germany
Last seen: 17 years, 6 months
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Re: Info on Pan cyan Florida [Re: B.I.O.]
#87011 - 08/11/00 07:07 PM (23 years, 1 month ago) |
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Hi Prellgott,no it doesn't really stink. It smells almost like a mixture of hay and soil, with a small touch of horse. I sterilized the shit in jars...
Elektrolurch
--------------------
"For all the time spent in that room
The doll's house, darkness, old perfume
And fairy stories held me high on
Clouds of sunlight floating by.", Pink Floyd '67
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Elektrolurch
enthusiast
Registered: 05/01/00
Posts: 307
Loc: Germany
Last seen: 17 years, 6 months
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Re: Info on Pan cyan Florida [Re: B.I.O.]
#87012 - 08/12/00 05:50 AM (23 years, 1 month ago) |
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Hi,here are some pics of the mycellium. The big petridishes are a multispore germination, while the two small ones are after selecting it many times. The dishes are old, so they look a little bit blued and some got a slimy contam at the edges of the dish. I also made photos of the back side of the big dishes, so you can see how the mycellium grows into the agar... Elektrolurch      
--------------------
"For all the time spent in that room
The doll's house, darkness, old perfume
And fairy stories held me high on
Clouds of sunlight floating by.", Pink Floyd '67
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Anonymous
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Re: Info on Pan cyan Florida [Re: B.I.O.]
#87013 - 08/12/00 06:57 AM (23 years, 1 month ago) |
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quote:
Originally posted by Shaman1:
Hey mycomountain, what did you grow those baby's in? dung, dung/compost, compost, straw?
My friend used straw and fresh horse shit.
------------------
Check out the new site
Quality spore prints & gourmet mushroom cultures
http://www.homestead.com/mycomountain
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Anonymous
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Re: Info on Pan cyan Florida [Re: B.I.O.]
#87014 - 08/13/00 03:17 AM (23 years, 1 month ago) |
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I don't claim to be an expert... but if I saw that stuff growing in a jar, or on some cheese, my first and only thought would be: mold. =\
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Elektrolurch
enthusiast
Registered: 05/01/00
Posts: 307
Loc: Germany
Last seen: 17 years, 6 months
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Re: Info on Pan cyan Florida [Re: B.I.O.]
#87015 - 08/14/00 03:58 AM (23 years, 1 month ago) |
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Not everything can be mold. It's a little bit difficult to see in the photos, that much of the mycellium got with time blue...
Elektrolurch
--------------------
"For all the time spent in that room
The doll's house, darkness, old perfume
And fairy stories held me high on
Clouds of sunlight floating by.", Pink Floyd '67
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B.I.O.
old hand
Registered: 05/26/99
Posts: 458
Last seen: 7 months, 3 days
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Re: Info on Pan cyan Florida [Re: B.I.O.]
#87016 - 08/18/00 12:49 AM (23 years, 1 month ago) |
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hard to say....i know that the mycelium easily turns blue from condensed water in the petri-dishes...i can send you a petri with a strong mycelium if you like....BiO.
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Anonymous
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Re: Info on Pan cyan Florida [Re: B.I.O.]
#87017 - 08/18/00 02:19 PM (23 years, 1 month ago) |
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Pan cyans are very proned to contamination. I have had dishes get the green mold while sitting in front of a HEPA. All of my other cultures were fine except the pan cyans... I can figure it out. Try using anti-biotic agar ad throw a bit of dung juice into the mix.
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mjshroomer
Sage
Registered: 07/21/99
Posts: 13,774
Loc: gone with my shrooms
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Re: Info on Pan cyan Florida [Re: B.I.O.]
#87018 - 08/19/00 01:15 PM (23 years, 1 month ago) |
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Hi Mycomountain,great photos of the Copelandia mushrooms. I think those are cool. have a shroomy day. And to all , remember I will be collecting Copelandia cyanescens from Cambodia in a few weeks and when I return I will have sporeprints available. have a nother shroomier day. Mjshroomer
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Ryche Hawk
A Messenger
Registered: 03/01/01
Posts: 2,112
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Re: Info on Pan cyan Florida [Re: B.I.O.]
#87019 - 08/21/00 08:18 PM (23 years, 1 month ago) |
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Panaeolus mycelium does look very different then cubensis mycelium. I cant tell you how many jars I threw out for many months before realized that pan mycelium just grows very strange looking. Very wispy, sometimes what looks like pinning in jars. On agar it looks very unhealthy.
For best results just inoc some rice/verm or birdseed/verm jars, let them run their course. Then break those up and use as spawn to inoc some dung or compost. Compost seems to work best for warding on contamination, which pan mycelium contaminates easily.
Strangly enough, the old dung doesn't stink.And easy way I used to sterilize it is put in an old pillow case, tie in a knot, and pasturize between 160-180F for 1 hour. Then let it hang outside for day to drain. When ready to mix with substrate, take handfulls and squeeze any excess water out to where its barely dripping. Mix with our broken spawn, put casing layer over it immediatly. It will usually fruit within 3 weeks. Use a light casing layer, about 1/4" and something like the 50/50+ mix with some lime in it works well.
The high from these is incredable. Well worth the efforts and frustrations and loosing some patches to contams.
BTW, nice patches there mycomountain. -peace- www.thehawkseye.com
--------------------
-Peace-
High Quality MUSHROOM SPORES and CULTURES for microscopy at www.muShrooms.com
muShrooms.com is the new web site of
www.thehawkseye.com
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Anonymous
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Re: Info on Pan cyan Florida [Re: B.I.O.]
#87020 - 08/22/00 05:42 AM (23 years, 1 month ago) |
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Again... I'm no expert (by far), but those pics look very much like the color pic or Aspergillus in TMC... Promisingly, though, your pics look a little more blue and a little less green.  I guess the only way to find out is to drop the agar in some jars, wait, and smell.  Good luck!
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Elektrolurch
enthusiast
Registered: 05/01/00
Posts: 307
Loc: Germany
Last seen: 17 years, 6 months
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Re: Info on Pan cyan Florida [Re: B.I.O.]
#87021 - 08/21/00 10:16 PM (23 years, 1 month ago) |
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Thanx for the info. I'll keep you informed if something works...
Elektrolurch
--------------------
"For all the time spent in that room
The doll's house, darkness, old perfume
And fairy stories held me high on
Clouds of sunlight floating by.", Pink Floyd '67
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