Home | Community | Message Board
You are not signed in.
Sign In New Account
Forum Index Search Posts Trusted Vendors Highlights Galleries FAQ User List Chat Store Random Growery

Magic-Mushrooms-Shop.com
This site includes paid links. Please support our sponsors.

Mushrooms, Mycology and Psychedelics > Gourmet and Medicinal Mushrooms
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Liquid Cultures   Sporeworks.EU Spores for European Microscopy   Myyco.com Golden Teacher Liquid Culture For Sale   MagicBag.co All-In-One Bags That Don't Suck   North Spore Bulk Substrate

Jump to first unread post Pages: 1 | 2 |  [ show all ]
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
. *DELETED* *DELETED*
    #8552417 -

Post deleted by wisp

Reason for deletion: .


Edited by wisp (09/08/11 10:26 PM)

Extras: Filter Print Post Top
Re: Failed cloning from button mushroom [Re: wisp]
    #8553078 -

Same here tripsis ;(

I made about 5 clones, non of them survived - same story as yours!

Then i made spore print from shop button and as you know sterility in shops is 0% so around most spores became contam!
Other spores started germinating only after 3 week :O :S

Well maybe that the secret of comerical shops for shrooms xD


--------------------
What is a mind, if not something to be messed with? What is consciousness, if not a state to be altered?

~I Feel
:mushroom2:
at Home~

Extras: Filter Print Post Top
Re: Failed cloning from button mushroom [Re: dstark]
    #8553081 -

You actually got button mushrooms spores to germinate? Wow, that's supposed to be really difficult. I took some fresh prints so I'm going to put some spores on agar this week. Hopefully I'll be able to get them to germinate too.:)

Extras: Filter Print Post Top
Re: Failed cloning from button mushroom [Re: wisp]
    #8553121 -

GL but be ready to wait :smile:


--------------------
What is a mind, if not something to be messed with? What is consciousness, if not a state to be altered?

~I Feel
:mushroom2:
at Home~

Extras: Filter Print Post Top
Re: Failed cloning from button mushroom [Re: dstark]
    #8554266 -

tripsis,

Maybe you should try a different agar medium - Say if you used PDA then use some MA or MYA.

I once got some button mushroom spawn (a few grains) and tried it on PDA - Very very very slow growth....

The agar plates dried out before the culture could get going  properly.

Extras: Filter Print Post Top
Re: Failed cloning from button mushroom [Re: primordius77]
    #8653741 -

what was the source of your starting material? (ex. spores from xxx, culture from xxx, ect.)

theres probably not a whole lot of agaricus cultivators on this forum I remember reading posts of RR saying he couldn't get his to fruit when he needed them for a video.. that made me not want to try growing agaricus mushrooms.


--------------------
coda said:
imachavel, Man you really need to do some reading, the amount of bullshit you put into almost every single one of your posts is absolutely astounding.

Extras: Filter Print Post Top
Re: Failed cloning from button mushroom [Re: primordius77]
    #8654109 -

primordius77 said:
tripsis,

Maybe you should try a different agar medium - Say if you used PDA then use some MA or MYA.

I once got some button mushroom spawn (a few grains) and tried it on PDA - Very very very slow growth....

The agar plates dried out before the culture could get going  properly.


Dried :/ well if you put parafilm they wont :smile:
Well i used also PDA and was very very slow! RR is cloning on agar with malt extract, i supose its batter ^^


--------------------
What is a mind, if not something to be messed with? What is consciousness, if not a state to be altered?

~I Feel
:mushroom2:
at Home~

Extras: Filter Print Post Top
Re: Failed cloning from button mushroom [Re: wisp]
    #8656543 -

How long have the chunks of mushroom been on agar?, sometimes bisporus takes 10 to 14 days before you see growth.

Extras: Filter Print Post Top
Re: Failed cloning from button mushroom [Re: falcon]
    #8657128 -

huh, Clones was failed, germination was from spores xD
took almost 1 month to germinate hahah xD lol!
then the agar was dry :frown:


--------------------
What is a mind, if not something to be messed with? What is consciousness, if not a state to be altered?

~I Feel
:mushroom2:
at Home~

Extras: Filter Print Post Top
Re: Failed cloning from button mushroom [Re: dstark]
    #8657342 -

Yeah, I posted a bit prematurely. It worked in the end, not all the pieces of tissue, but several. Still have it all on plates though,growth is so slow. I'll start some jars of spawn tomorrow though.

Extras: Filter Print Post Top
Re: Failed cloning from button mushroom [Re: wisp]
    #8667582 -

maybe if you did the stab the stem tek and made a lc clone it work better


--------------------
might show tits for prints ;o)

Extras: Filter Print Post Top
Re: Failed cloning from button mushroom [Re: C. duckie]
    #8670703 -

I don't see how it would make a difference how the tissue was taken from the mushroom. As I said, it did work out, it's just Agaricus mycelium is very slow.

I'm not keen on the idea on cloning straight to liquid culture. I prefer cloning to agar to ensure the culture is clean, then proceeding to LC later.

Extras: Filter Print Post Top
Re: Failed cloning from button mushroom [Re: wisp]
    #8670828 -

"I'm not keen on the idea on cloning straight to liquid culture. I prefer cloning to agar to ensure the culture is clean, then proceeding to LC later. "


Good thinking.

Extras: Filter Print Post Top
Re: Failed cloning from button mushroom [Re: wisp]
    #8706433 -

tripsis said:
Hi, I have been growing some button mushrooms. As I want to continue this I decided to clone off one. I did so yesterday and placed a small piece of tissue of several agar plates. Unexpectedly though, the tissue has turned brown and appears to have died.

My question is, is this somehow due to senescence of the strain because it is commercially grown? I tried cloning off shop bought button mushrooms with the same results, but I attributed the failure to the fact the mushrooms were days old. This one was most definitely alive when I cloned it.

Is it normal for it to go brown like that and can I still expect to see growth at some point?

Thanks for any feedback.


I have tried cloning some other mushrooms on agar before and quite often, the tissue would turn brown and soggy and look dead but after a while, mycelium will grow from it.  Sometimes however, it will not grow mycelium.  Just my personal experience.


--------------------
HAVE: Paneolus cyanescence, Paneolus cambodgiensis, Psilocybe cubensis, Flammulina velutipe (white, and brown Korean strain), Hericium erinaceous, Coprinus atramentarius, Coprinus comatus, Agrocybe aegerita, Pleurotus eryngii, Psilocybe subaeruginosa, Ganoderma lucidum, Pleurotus nebrodensis, Lepista nuda, Agaricus augustus, Pleurotus cystidiosus, Volvariella speciosa.

LOOKING FOR: Those that I don't have.

Extras: Filter Print Post Top
Re: Failed cloning from button mushroom [Re: cutemushie]
    #8711477 -

Same here :smile:


--------------------
What is a mind, if not something to be messed with? What is consciousness, if not a state to be altered?

~I Feel
:mushroom2:
at Home~

Extras: Filter Print Post Top
Re: Failed cloning from button mushroom [Re: dstark]
    #8713695 -

Yeah, it was quite strange actually. Many of the pieces of tissue that went brown, ended up showing growth, while the pieces that stayed firm and white did not.

Extras: Filter Print Post Top
Re: Failed cloning from button mushroom *DELETED* [Re: wisp]
    #8721898 -

Post deleted by lorbitherize

Reason for deletion: ...


Extras: Filter Print Post Top
Re: Failed cloning from button mushroom [Re: lorbitherize]
    #8721956 -

:thumbup:

I thought by myself the PDA arnt the best option for edibles, well i saw RR recomend malt extract agar.


--------------------
What is a mind, if not something to be messed with? What is consciousness, if not a state to be altered?

~I Feel
:mushroom2:
at Home~

Extras: Filter Print Post Top
Re: Failed cloning from button mushroom [Re: dstark]
    #8748138 -

My button mushroom clone didn't work either (on PDA), since it is a dung lover I'd suggest sterilizing some horse manure in a jar and throwing your tissue clone in there.Experiment with water content for best results.

:jester:


--------------------
Give a man a fire and he will be warm for the rest of the night.
Set a man on fire and he will be warm for the rest of his life.
NEWB NEWBIE NEWCOMER IGNORANT? QUESTIONS?
Click HERE HERE HERE HERE For detailed instructions with pictures on how to grow mushrooms. There is a lot of info on the Shroomery and this is what you need to know.

Extras: Filter Print Post Top
Re: Failed cloning from button mushroom [Re: Peterthinks]
    #8748318 -

PDA works fine for me with every mushroom i've cultured, Agaricus' included.

Extras: Filter Print Post Top
Jump to top Pages: 1 | 2 |  [ show all ]

Shop: Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Liquid Cultures   Sporeworks.EU Spores for European Microscopy   Myyco.com Golden Teacher Liquid Culture For Sale   MagicBag.co All-In-One Bags That Don't Suck   North Spore Bulk Substrate

Mushrooms, Mycology and Psychedelics > Gourmet and Medicinal Mushrooms
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Similar ThreadsPosterViewsRepliesLast post
* Washing machine tek poboy 1,240 5 10/11/05 12:07 AM
by Dragonaut
* Do you like edibles becuase of actives?
( 1 2 all )		 whole9 5,024 24 11/26/03 01:26 PM
by Werecat
* Medicinal Mushrooms Prometheus_Unbound 2,076 6 02/06/03 09:08 PM
by G a n j a
* g&mm special eric_the_redS 3,513 19 02/11/04 03:04 PM
by eric_the_red
* gourmets to grow with PF Tek *DELETED* adeeplust 1,566 5 01/27/07 04:22 PM
by Dety
* Post deleted by Administrator
( 1 2 3 all )		 i am e goldstein 10,123 45 03/01/03 08:05 AM
by MushMushi
* I got pins!! Shaggy Mane *DELETED*
( 1 2 all )		 Morelman 4,543 27 02/15/07 01:08 PM
by Workman
* Pink Oyster Puffing-Up! *DELETED* lorbitherize 2,251 17 01/05/07 03:57 PM
by Hotnuts

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: RogerRabbit, cronicr, Pastywhyte, Forrester, Stromrider, SHROOMSISAY01
4,148 topic views. 0 members, 3 guests and 6 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2026 Mind Media. Some rights reserved.

Generated in 0.026 seconds spending 0.004 seconds on 16 queries.