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Offlinemikearch
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Registered: 08/21/02
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spore cap
    #835153 - 08/22/02 06:44 AM (14 years, 3 months ago)

i have one very very very small cap and i was wondering if, while waiting for my next batch of spore syringes i could cut this cap in pieces with sterile scissors and put it in sterile water to make a syringe so that i can start another couple jars to experiment with, or would there be too many contaminants on the cap? and if there is, is there any way to sterilize it w/out kiling the spores? sorry for all o the questions.
thanks
mike


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InvisibleDreaMaTrix
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Re: spore cap [Re: mikearch]
    #835169 - 08/22/02 06:50 AM (14 years, 3 months ago)

Why don't you make a very very small spore print from the cap? Then proceed with your plan. There will be more than enough spores to make a syringe

Good luck



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"We are the one's we have been waiting for" - Hopi saying


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OfflineNecron99
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Re: spore cap [Re: mikearch]
    #835303 - 08/22/02 08:10 AM (14 years, 3 months ago)

Gotta agree with Dream'.
Just make a print. Then make your syringe.



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"Puny earthlings do not yet know the meaning of the word suffering!!"



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OfflineMacey Howard
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Post deleted by Moe Howard [Re: DreaMaTrix]
    #835319 - 08/22/02 08:21 AM (14 years, 3 months ago)



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Offlinemikearch
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Re: spore cap [Re: Macey Howard]
    #835374 - 08/22/02 08:48 AM (14 years, 3 months ago)

ok i forgot to mention the cap is dried, for who knows how longbecause it was from a bag of shrooms i purchased about 3 weeks ago and it is about 1/2 cm or 5 mm wide.
i am going to try to get a spore print in a sterile jar from it right now and if i don't have one by tomorrow would it be too un sterile to chop it up like i said above?


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OfflineNecron99
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Re: spore cap [Re: mikearch]
    #835379 - 08/22/02 08:52 AM (14 years, 3 months ago)

If you got it out of a bag you bought, chances are it's not the slightest bit sterile right now.

You're gonna have to wait for the mail....



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"Puny earthlings do not yet know the meaning of the word suffering!!"



Edited by Necron99 (08/22/02 08:54 AM)


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Invisiblemickey_rourke
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Re: spore cap [Re: mikearch]
    #835584 - 08/22/02 11:10 AM (14 years, 3 months ago)

If you want to do something with that cap (unfortunately, based on your experience level, I feel that you would fail if you follow my advice), you can powder it up and streak some agar plates with it. You will undoubtedly suffer from contamination, but with agar, you have the ability to transfer good growth to new plates to grow out.


--------------------
"I tried to put it all behind me, but my redneck past is nipping at my heels.." -- Ben Folds Five


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Offlinemikearch
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Re: spore cap [Re: mickey_rourke]
    #835651 - 08/22/02 11:40 AM (14 years, 3 months ago)

thanks for the idea mickey, any suggestions as to where to locally get the agar plates or any substitute to use for agar? this is just something i wanted to do to entertain myself and learn while waiting on syringes.


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Invisiblemickey_rourke
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Re: spore cap [Re: mikearch]
    #835664 - 08/22/02 11:48 AM (14 years, 3 months ago)

In reply to:

thanks for the idea mickey, any suggestions as to where to locally get the agar plates or any substitute to use for agar?


You can buy agar agar at Asian or health food stores. Ask for agar agar. They will know what it is. Look in the FAQs for a good agar recipe so that you know what to mix with the agar. Concerning the petri dishes, either buy them, or use something like a mason jar.

Good luck!


--------------------
"I tried to put it all behind me, but my redneck past is nipping at my heels.." -- Ben Folds Five


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Offlinemikearch
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Re: spore cap [Re: mickey_rourke]
    #835815 - 08/22/02 12:54 PM (14 years, 3 months ago)

thanks a bunch mickey


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Amazon Shop for: ½ Pint Jars, Agar, Petri Dish

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