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InvisibleTestertips
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4 year old spores on agar not germinating - over 2 weeks
    #25050691 - 03/09/18 12:32 AM (6 months, 8 days ago)

toss or wait?


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OfflineMooseShroom
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Re: 4 year old spores on agar not germinating - over 2 weeks [Re: Testertips]
    #25050693 - 03/09/18 12:34 AM (6 months, 8 days ago)

I'd try again with softer agar.


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InvisibleTestertips
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Re: 4 year old spores on agar not germinating - over 2 weeks [Re: MooseShroom]
    #25050697 - 03/09/18 12:38 AM (6 months, 8 days ago)

Quote:

MooseShroom said:
I'd try again with softer agar.




been successfully using Pasty's tek for years - did not deviate this round.  it has been a bit cold lately though.


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OfflineMooseShroom
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Re: 4 year old spores on agar not germinating - over 2 weeks [Re: Testertips]
    #25050728 - 03/09/18 01:08 AM (6 months, 8 days ago)

In that case I'd keep the plate to see if anything pops up eventually and give it another shot. Do what works well for you :thumbup:


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Re: 4 year old spores on agar not germinating - over 2 weeks [Re: MooseShroom] * 1
    #25050794 - 03/09/18 02:12 AM (6 months, 8 days ago)

Brf plates are good for stubborn spores


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OfflineBaby_Hitler
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Re: 4 year old spores on agar not germinating - over 2 weeks [Re: Testertips]
    #25050815 - 03/09/18 02:28 AM (6 months, 8 days ago)

I've got some 6 year old spores on especially stiff agar right now. (14g/500ml, Telephone brand) It's been 4 days. Not sure I want to wait 6 weeks to see if anything happens. Thinking about doing some more with, like, 7g/500ml.


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InvisibleTestertips
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Re: 4 year old spores on agar not germinating - over 2 weeks [Re: Baby_Hitler]
    #25052047 - 03/09/18 03:48 PM (6 months, 7 days ago)

Quote:

Baby_Hitler said:
I've got some 6 year old spores on especially stiff agar right now. (14g/500ml, Telephone brand) It's been 4 days. Not sure I want to wait 6 weeks to see if anything happens. Thinking about doing some more with, like, 7g/500ml.




Let me know if they germinate and how long it took... thanks.


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InvisibleHumble Newcomer
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Re: 4 year old spores on agar not germinating - over 2 weeks [Re: Testertips]
    #25052062 - 03/09/18 03:53 PM (6 months, 7 days ago)

I just put a five year old print to agar. my standard recipe, did a streak plate with a loop.

Nothing but small orange bacterial spots for the first 6 days or so then only two tiny spots of myc formed from the whole plate. I transferred them both after about 10 days.

Another avenue I've heard people having success with is making a syringe from the print, this helps hydrate the spores before knocking up a plate. There's even threads on forced hydration, leaving needle off and capping syringe and weighing down plunger to create positive pressure inside over night


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OfflineCanharo
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Re: 4 year old spores on agar not germinating - over 2 weeks [Re: Humble Newcomer]
    #25052200 - 03/09/18 04:55 PM (6 months, 7 days ago)

I don't know how to link it but look up Stro's spore salvage with forced hydration & Stro's Spore Salvage with Malt Yeast Carbon Agar (MYCA) :thumbup:  I've done/ been doing this with syringes and prints 5 years old or so and I'm experimenting with one as old as 10 years
(edited for highness)


Edited by Canharo (03/09/18 04:57 PM)


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InvisibleZiranS
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Re: 4 year old spores on agar not germinating - over 2 weeks [Re: Canharo] * 2
    #25052219 - 03/09/18 05:02 PM (6 months, 7 days ago)

Stro's spore salvage with forced hydration & Stro's Spore Salvage


You can try the rubber band trick too.

Same principle as this thread here Stro's Spore Salvage with Forced Hydration

Except you wrap a rubber band long ways to hold the plunger tight making pressure.


--------------------
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Pastywhyte said:
I'm probably just being picky but usually a good tek has photos illustrating the process from beginning to end :shrug:


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InvisibleHumble Newcomer
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Re: 4 year old spores on agar not germinating - over 2 weeks [Re: Ziran]
    #25052230 - 03/09/18 05:06 PM (6 months, 7 days ago)

Is there a place to ask stupid wuestions like how to make hyperlinks and how to remove certain threads from your "my threads" and why some people have two rows of shrooms on their name instead of just one row?

If there is such a place no one prob frequents it to answer such queries


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InvisibleZiranS
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Re: 4 year old spores on agar not germinating - over 2 weeks [Re: Humble Newcomer] * 1
    #25052235 - 03/09/18 05:09 PM (6 months, 7 days ago)

Quote:

Humble Newcomer said:
Is there a place to ask stupid questions like how to make hyperlinks and how to remove certain threads from your "my threads" and why some people have two rows of shrooms on their name instead of just one row?

If there is such a place no one prob frequents it to answer such queries




general rating and trade rating

the little box here





--------------------
Song Of Healing
Ziran's Teks
:super: Updated Pf Tek Guide :super:
AMU Q&A Thread


Pastywhyte said:
I'm probably just being picky but usually a good tek has photos illustrating the process from beginning to end :shrug:


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InvisibleHumble Newcomer
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Re: 4 year old spores on agar not germinating - over 2 weeks [Re: Ziran]
    #25052275 - 03/09/18 05:29 PM (6 months, 7 days ago)

Sweet! And how to remove a thread I don't want to see on my threads anymore please anyone?

Sorry for thread jack


--------------------
My name is not a proclamation but a reminder of how to carry myself.

How I made my mush GH                              How I make chocolates

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InvisibleTestertips
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Re: 4 year old spores on agar not germinating - over 2 weeks [Re: Ziran]
    #25053008 - 03/09/18 10:02 PM (6 months, 7 days ago)

Quote:

Ziran said:
Stro's spore salvage with forced hydration & Stro's Spore Salvage


You can try the rubber band trick too.

Same principle as this thread here Stro's Spore Salvage with Forced Hydration

Except you wrap a rubber band long ways to hold the plunger tight making pressure.




thanks dude!


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Invisibleelasticaltiger
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Re: 4 year old spores on agar not germinating - over 2 weeks [Re: Testertips]
    #25053075 - 03/09/18 10:26 PM (6 months, 7 days ago)

Toss or keep it somewhere else. Aint no one got time for that.


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Re: 4 year old spores on agar not germinating - over 2 weeks [Re: elasticaltiger]
    #25053100 - 03/09/18 10:33 PM (6 months, 7 days ago)

Quote:

elasticaltiger said:
Toss or keep it somewhere else. Aint no one got time for that.




:whippedcreamhead:


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