Psilocybe cubensis

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Olgualion
Shaman-In-Training

Registered: 11/14/01
Posts: 1,253
Loc: Currently Earth...

Psilocybe cubensis "B+" on horse manure

#750094 - 07/16/02 07:31 AM (24 years, 3 months ago)

This will be a grow log of a tray of grain spawned (microwave pasteurised) manure.

Here is a box of some of the manure. It has been sitting out in dude?s backyard for the past few weeks.

It was broken up finely into a caserole pan, and it then had some boiling water poured over it (well beyond saturation). Then microwaving began. 700W microwave for about 8 mins, stirred, and nuked again for another 4 or 5. This was removed, stirred, and covered with foil. No care was taken in making sure of proper water content(it was squeezed to the proper level after cooled).

Then on the morning of the 15th, the excess h2o was squeezed out and the manure laid into an aluminum pan.
this was spawned with a quart of fully colonized B+ on millet.

24 hours after spawning:

I will update this log as I find time. I have been busy lately, and it is a pain to have to go over to dude?s house for the pics.

I don?t even know if this will work, as all other attempts with bulk substrate have been pasteurized using the pillow-case method for 2 hours. It may totally succumb to mold in the next few days, we will see...

-----
7/17 update

Here is the tray 24 hours later:

------
7-18 update

24 hours or so later
Going nicely:

Dude may case tonight...

-----
7-19 update

Here is a pic from early this morning:

And this one from this evening:

And a close up, just before casing. Notice the one spot of uncolonized dung which keeps exuding water.

Dude cased tonight with 50-50 which was microwave pasteurized then sprayed to capacity with peroxidated H2O. Tray was covered with AL foil then placed back on the shelf.

-----------
update 7-22

Here?s what dude woke up to this morning.

I told him he should have patched the first areas in the upper right hand corner yesterday, but he is a lazy ass. Anyway, he sprinkled a little casing over the areas that are overgrown (like the upper right),and he sprayed the entire surface, well. Then he gave one good fanning, and covered the tray well (it is going into the dirty fridge afterall). Then he placed it into the fridge. He will take it out and put it into the fruiting chamber either tonight of tomorrow morning, depending upon how "tired he is".

----
updated 7-23

dude took the casing out of the fridge last night(monday 22nd), and put it under fruiting conditions (or as close as possible- it has been in the 90?s lately.. yuck...)

We will see if the temp being so high will have a negative effect on pinning/fruiting. This strain normally pins in 3 or 4 days after cold shock. or up to 10 days w/o cold shock. we will see though.

Already tonight there is a bunch more colonisation of the casing layer.... not a good sign... It will probably result in a less than perfect pinset(something dude is not used to with this strain), and a reduced yield. I can?t wait for Fall...

-------
update 7-24

Here is a pic of the casing in the fruiting chamber this morning.

can?t make out the rhizomorphs very well, but they are reaching up from the casing, all over the tray. Hopefully we will see a bunch of knots by friday evening...

------
update 7-25

Here?s a pic from this morning.

There are a few clusters of hyphal knots around the tray dude said. But he was in a rush, and did?nt have time to take pics of them. The casing is right on schedule, actually a few hours ahead of his (usual) isolate. Not bad, he took the casing out of c.s. on the night of the 22nd, and can already see knots on the morning of the 25th.

This should turn out to be a very nice pinset/flush afterall...

-----
update 7-26

Well, pins galore... near perfect pinset. They are very small now, but within the next ten or so hours he should be able to get a good pic of them. Within the next few hours, he will drop the humidity for fruiting.

more to come soon...

----
update 7-27

as of 10:00 AM

I don?t know what is wrong with the camera... The settings must?ve been changed, and haven?t had the time to tweak them...

about 12 hrs later...

wait till tomorrow morning....

----
update 7-28

odd, I thought I updated this this morning... oh well.. here is about 24 hours since the last pic:

------
update 7-29

here they are about 12 hours past the last pic..

and a slightly different angle..

some of the veils have broken, but they are not quite ready for printing. Dude has work today, and he doesn?t want to come home to a tray full of spores... decisions, decisions... maybe he will pick all but the smallest, and hope that they will be where he wants them, by the time he gets home.

---
a bunch were picked right after the previous pic (the right side of the tray), so they wouldnt drop all over the place.

about 12 hrs later. I thought the others still may sporulate, and dude would come home to a black tray, but they ended up maturing just right in those 12 hrs (maybe a little over done)...

he has 26 caps which are printing. 6 or which already have a home.

-----
conclusion:

nuked horse manure works very well, and is much easier than the pillowcase pasteurisation method. Altogether, from spawning to harvest it took 2 weeks. Spawned on the 14th or 15th, and harvested on the 29th. The only problem that I see there is no way to add straw, if you want a straw/manure combo.

I was thinking of having this be an ongoing grow log including following flushes, making clones, and whatever else comes to mind. But I don?t think so...

Anyway, hope you enjoyed the log, and if you have any Q?s? Ask here...

--------------------
Study the past...
See the future...

Edited by Annom (12/21/04 12:55 PM)

Extras:

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