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 Joshua
Holoman
Registered: 10/27/98
Posts: 5,398
Loc: The Matrix
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 Psilocybe cubensis "Un Id" isolate on straw/compost cased. 
#601017 - 04/07/02 12:53 AM (23 years, 2 months ago) |
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This is Un Id colonised rye. I isolated fruit bodies off of a multispore agar plate from what apeared to be a healthy strain from what I will call the Un Id strain. This jar was inoculated with an agar transfer on 3/28. This is my first rye jar that did not develope a bacterial contam. I am contemplating on what to do with it. I have considered spawning straw w/ it or possibly some more compost, maybe my tastey mint compost.
This strain feels good to me. I have noticed strong growth from the original multispore through all of my isolations. I hope to be able to distribute spore prints from it soon.
Original isolation from multispore:
Second isolation:
Agar plate pinning from second isolation:
Transfered fruits from previous plate to new plate. This is after 7 days:
I dont have a final pic from the previous plate once it grew out, but I can asure you it was a beautiful site.
Here is the colonised rye grain after 10 days:
All in all, three isolations were done from the original multispore. BTW..this is my first attempt at fruiting from an agar isolation.
Joshua
--------------------
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Edited by concretefeet (01/23/05 02:00 AM)
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 weatstraw
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 Re: Un Id strain from the wilds of Florida. [Re: Joshua]
#601027 - 04/07/02 01:13 AM (23 years, 2 months ago) |
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looks like a piece of tyvek under the lid there, is that it? I?d like to know since I have an everlasting supply of tyvek postal mailing bags. I?d like to know how well they work in actual use.
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Joshua
Holoman
Registered: 10/27/98
Posts: 5,398
Loc: The Matrix
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 Re: Un Id strain from the wilds of Florida. [Re: weatstraw]
#601035 - 04/07/02 01:30 AM (23 years, 2 months ago) |
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No, its not Tyvek per se. It is similar though. I got the filter from a trade. Anno has had great luck w/ Tyvek. Use it by placing over the mouth of the jar, place lid and then ring over the top of that. If you need further explaination let me know.
Joshua
--------------------
The Shroomery Bookstore
Great books for inquiring minds!
"Life After Death is Saprophytic!"
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Roadkill
Retired Shroomery Mod
Registered: 12/11/01
Posts: 22,674
Loc: Montana
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 Re: Un Id strain from the wilds of Florida. [Re: Joshua]
#601052 - 04/07/02 03:13 AM (23 years, 2 months ago) |
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looking very nice bro !!!
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Laterz, Road
Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!
Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.
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DazedSol
old hand
Registered: 08/01/01
Posts: 1,230
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Re: Un Id strain from the wilds of Florida. [Re: Joshua]
#601371 - 04/07/02 02:15 PM (23 years, 2 months ago) |
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Hey wow....is that from the print i sent you?
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Peace,
Adam
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Joshua
Holoman
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Re: Un Id strain from the wilds of Florida. [Re: DazedSol]
#601450 - 04/07/02 04:23 PM (23 years, 2 months ago) |
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I believe it is. I will send you a print when they fruit if you remind me.
Micron-
I don?t see the point of trimming the filters, suppose I want to use them w/ gallon jars in the future.
Joshua
--------------------
The Shroomery Bookstore
Great books for inquiring minds!
"Life After Death is Saprophytic!"
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Joshua
Holoman
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Re: Un Id strain from the wilds of Florida. (4/8) [Re: Joshua]
#601802 - 04/08/02 01:02 AM (23 years, 2 months ago) |
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I decided on spawning this w/ a modified Dr. Bluethumbs compost. I took his compost and mixed it with straw, vermiculite, and spawn mate from his site. I sterilised this in the oven at 350 F for 1 hr in an oven bag. container is a diaper wipes container. Substrate is a little over two inches deep.
Joshua
--------------------
The Shroomery Bookstore
Great books for inquiring minds!
"Life After Death is Saprophytic!"
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Joshua
Holoman
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Posts: 5,398
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Re: Un Id strain from the wilds of Florida. (4/11) [Re: Joshua]
#605458 - 04/11/02 02:02 PM (23 years, 2 months ago) |
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4/11 Mid-day
Joshua
--------------------
The Shroomery Bookstore
Great books for inquiring minds!
"Life After Death is Saprophytic!"
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Banshee
wannabepsilocybe
Registered: 06/23/01
Posts: 402
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Re: Un Id strain from the wilds of Florida. (4/11) [Re: Joshua]
#605619 - 04/11/02 05:16 PM (23 years, 2 months ago) |
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That mycelium is... I could lick that tray empty...
keep on posting us = D
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The Universal Spirit is within.
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Food
---Beast---
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Re: Un Id strain from the wilds of Florida. (4/11) [Re: Joshua]
#605628 - 04/11/02 05:28 PM (23 years, 2 months ago) |
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Very Very very nice indeed - I like pictures of Agar with mycelium in , nice one - keep it up boar .
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--------mushworld.com-----More info than you can throw a stick at-
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Joshua
Holoman
Registered: 10/27/98
Posts: 5,398
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Re: Un Id strain from the wilds of Florida. (4/14) [Re: Joshua]
#608551 - 04/14/02 11:18 PM (23 years, 2 months ago) |
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4/14 late evening
I have cased this tray. I hope to show some pins soon.
Joshua
--------------------
The Shroomery Bookstore
Great books for inquiring minds!
"Life After Death is Saprophytic!"
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Kindnug
member
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Re: Un Id strain from the wilds of Florida. (4/14) [Re: Joshua]
#609692 - 04/16/02 03:26 AM (23 years, 2 months ago) |
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Nice work mon, I hope you get a huge first flush!!
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Split
enthusiast
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Re: Un Id strain from the wilds of Florida. (4/14) [Re: Joshua]
#609716 - 04/16/02 04:46 AM (23 years, 2 months ago) |
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This one?s gonna blow up bro 
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[green]"You'll accomplish more if you start now"[/green]
[red]-Egghead[/red]
[green]TeRzMaStA[/green] is the man.
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tomldp
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Re: Un Id strain from the wilds of Florida. (4/14) [Re: Joshua]
#609736 - 04/16/02 05:52 AM (23 years, 2 months ago) |
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Very nice work Josh !
Can you give us more details on this strain (if you have some) ? Do you think it is a brand new strain ?
Looks very interesting and promising ! I?m really curious of the results...
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 Visit and support the Free Spore Ring Europe
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Joshua
Holoman
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Loc: The Matrix
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Re: Un Id strain from the wilds of Florida. (4/14) [Re: tomldp]
#609894 - 04/16/02 10:09 AM (23 years, 2 months ago) |
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I got the print from DazedSol. I think this is the first cultivation of this strain.
DazedSol, can you inform us of any details?
Joshua
--------------------
The Shroomery Bookstore
Great books for inquiring minds!
"Life After Death is Saprophytic!"
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babyshroom
addict
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Re: Un Id strain from the wilds of Florida. (4/14) [Re: Joshua]
#610656 - 04/17/02 08:14 AM (23 years, 2 months ago) |
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Yes, please do dazedsol. foaf considering some outdoor cultivation in the future. Do you know whereabouts in Fl these were found? I?m sure I?d have better luck growing outdoors if I started with a strain that already likes my area.
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I don't really know anyone who grows mushrooms. I just come here for fun.
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DazedSol
old hand
Registered: 08/01/01
Posts: 1,230
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Re: Un Id strain from the wilds of Florida. (4/14) [Re: Joshua]
#612028 - 04/18/02 02:57 PM (23 years, 2 months ago) |
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Hey yea......you guys know about as much as i do on these........they came from a friend down in Fort Myers.....thats all i know.....i have been trying to get ahold of my buddy down there for a while now......but to no avail......i?ll let you guys know if i find anything else out......they do seem to grow quiete large though....some of the prints i had of these were huge, the size of a minidisc.......
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Peace,
Adam
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Skankfeet
journeyman
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 Re: Un Id strain from the wilds of Florida. (4/14) [Re: Joshua]
#612316 - 04/18/02 08:31 PM (23 years, 2 months ago) |
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Hey Joshua ...ahmmm you do plan to print a few of those babys...I hope ...you might remember your buds...ahemmm....right....cause I have a feeling they are going to be fruitful and multiply hugely....when you do print some ..how bout tossing me a frisbee
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I'm never SURE of anything
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Joshua
Holoman
Registered: 10/27/98
Posts: 5,398
Loc: The Matrix
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Re: Un Id strain from the wilds of Florida. (4/14) [Re: Joshua]
#616305 - 04/23/02 11:23 AM (23 years, 1 month ago) |
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I had a set back. The casing material I added previously was contaminated. I have eliminated the contamination. This is what the compost looked like before I cased it a second time just now.
Here is a pic of UnId colonising maple chips.
Joshua
--------------------
The Shroomery Bookstore
Great books for inquiring minds!
"Life After Death is Saprophytic!"
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midnitesun
old-school,master-grower
Registered: 07/12/01
Posts: 216
Loc: East Coast(on the water-f...
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 Re: Un Id strain from the wilds of Florida. (4/14) [Re: Joshua]
#616357 - 04/23/02 12:29 PM (23 years, 1 month ago) |
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hey josh, nice werk so far,......i got some strong ass k.s. jars and bags colonizing....from syringes i bought from you.....a while back,(no contams either)...........keep it up.......peace
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"3 o'Clock Road Block,hey mister Cop, I ain't got no,Ber-surfer-ticket"(rfic). (Bob Marley)
"Here's an idea,lets make the Constitution legal". (Ron Paul)
"The war on drugs is a lie created by the State to enslave it's citizens".(midnitesun)
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angryshroom
Stranger
Registered: 12/18/01
Posts: 7,264
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Re: Un Id strain from the wilds of Florida. (4/14) [Re: Joshua]
#616435 - 04/23/02 01:46 PM (23 years, 1 month ago) |
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Dude, I dont understand, you?re unfortuently always getting contamination. Is it were you live, or what?
Sorry to hear about that, i hope we get to see the fruits of this Un ID strain
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SouthernGent
veteran
Registered: 01/12/02
Posts: 1,331
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 Re: Un Id strain from the wilds of Florida. (4/14) [Re: angryshroom]
#616453 - 04/23/02 02:09 PM (23 years, 1 month ago) |
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LOL angry I dont know if you been watching the mushroom pic forum much over the last several months or not but Josh dont always get contams... most of the time he gets beautiful shrooms
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Joshua
Holoman
Registered: 10/27/98
Posts: 5,398
Loc: The Matrix
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Re: Un Id strain from the wilds of Florida. (4/14) [Re: angryshroom]
#616777 - 04/23/02 08:31 PM (23 years, 1 month ago) |
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I am using the floor of a closet as my grow space. Unfortunately the floor is a wonderful place to find contams. I also beleive I have a high mold/spore count in my home. I am working on being more careful w/ my practices. This casing that contamed was from the mix itxelf. I pre-sterilise my casing mix. I had this one in a quart jar covered w/ foil. It turns out that I puctured a hole in the foil w/o noticing before cased my substrate w/ it. As soon as I can I will be moving my set-up to a better location. I have been keeping my eye open for cabinettes that would work. This summer I will have much more time on my hands since I will be on vacation from school. You should see some nice projects then.
SouthernGent-
Thank you for the support.
Joshua
--------------------
The Shroomery Bookstore
Great books for inquiring minds!
"Life After Death is Saprophytic!"
Edited by Joshua (04/23/02 08:32 PM)
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Roadkill
Retired Shroomery Mod
Registered: 12/11/01
Posts: 22,674
Loc: Montana
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 Re: Un Id strain from the wilds of Florida. (4/14) [Re: Joshua]
#616816 - 04/23/02 09:18 PM (23 years, 1 month ago) |
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Bro,
You could use a large rubbermaid to raise it off the floor.
Stick your growing chamber on top of the extra container.
Plus you could put some of your supplies in that unused container.
Like the ones that I use in my grow room. 
Just a thought!
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Laterz, Road
Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!
Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.
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Joshua
Holoman
Registered: 10/27/98
Posts: 5,398
Loc: The Matrix
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Re: Un Id strain from the wilds of Florida. (4/14) [Re: Joshua]
#620797 - 04/27/02 09:44 PM (23 years, 1 month ago) |
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I cased this tray 4 days ago.
This is the inside of my new terrarium, 33 gallons of pure fruiting chamber ready to grow some shrooms.
Joshua
--------------------
The Shroomery Bookstore
Great books for inquiring minds!
"Life After Death is Saprophytic!"
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Skankfeet
journeyman
Registered: 02/24/02
Posts: 74
Last seen: 21 years, 1 month
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Re: Un Id strain from the wilds of Florida. (4/14) [Re: Joshua]
#621605 - 04/28/02 06:40 PM (23 years, 1 month ago) |
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lookin good. Ready to see some frisbie size caps open up. Take pics and then prints of this lovely new strain...need to have a contest to name it ...I?ll kick something in if you do.
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I'm never SURE of anything
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Joshua
Holoman
Registered: 10/27/98
Posts: 5,398
Loc: The Matrix
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Re: Un Id strain from the wilds of Florida. (4/14) [Re: Joshua]
#621648 - 04/28/02 07:26 PM (23 years, 1 month ago) |
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They are not growing upwards. I thought my lid allowed enough diffuse light, but by the look at things I may need to improvise.
I also added additional casing to the tray. It may look like I took the second picture closer, but I scaled them both to be the same size.
Joshua
--------------------
The Shroomery Bookstore
Great books for inquiring minds!
"Life After Death is Saprophytic!"
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Joshua
Holoman
Registered: 10/27/98
Posts: 5,398
Loc: The Matrix
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Re: Un Id strain from the wilds of Florida. (4/14) [Re: Joshua]
#622690 - 04/29/02 11:07 PM (23 years, 1 month ago) |
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You are witnessing what I believe to be the first captive growing of this strain. The fruits are from a multispore isolated fruit off of agar...I hope that last part made sense.
A close-up of the larger fruit. This cap is just past prime pickin?s. If I would have caught it about 1/2 an hour ago it would have been perfect for a bioassay. I am choosing to print my fungi friend. Check back, I will post at which point to harvest the cap.
4 hrs later it is starting to drop its spores.
This is another small shroom developing a little behind the other two.
4 hrs later.
I know they?re all out of proportion. I am trying to leave them in the terrarium and not move them so much due to past contam problems. To get the angles right is a bit hard.
If anyone has software available that would allow me to post moving .gif? images I would appreciate it. Road, I?ll PM you about this, I think you said you might have the program I need.
I?m stoned...can you tell?
...and drinking some of the finest, Widmer Blonde Ale.
Joshua
--------------------
The Shroomery Bookstore
Great books for inquiring minds!
"Life After Death is Saprophytic!"
Edited by Joshua (04/30/02 02:43 AM)
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angryshroom
Stranger
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Posts: 7,264
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Re: Un Id strain from the wilds of Florida. (4/14) [Re: Joshua]
#622730 - 04/29/02 11:57 PM (23 years, 1 month ago) |
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Hey they are looking nice. I cant wait to see the fully matured fruits.
BTW--I didnt mean to sound harsh on my last posting, I wasnt trying to put you down at all or anything, I was just sad to see you getting contamination with this strain. 
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Joshua
Holoman
Registered: 10/27/98
Posts: 5,398
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Re: Un Id strain from the wilds of Florida. (4/14) [Re: angryshroom]
#622762 - 04/30/02 12:35 AM (23 years, 1 month ago) |
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You called it as you saw it...I see no problem in that.
I think I isolated part of my problem. I have been using a styrofoam cooler as my fruiting chamber. I think that the porous surface of the styrofoam harbored contaminants. I have since started using more H2O2 in my cultivation and have switched to a Rubbermaid. I will beat this and have wonderful fruits to show.
Joshua
--------------------
The Shroomery Bookstore
Great books for inquiring minds!
"Life After Death is Saprophytic!"
|
Roadkill
Retired Shroomery Mod
Registered: 12/11/01
Posts: 22,674
Loc: Montana
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Re: Un Id strain from the wilds of Florida. (4/14) [Re: Joshua]
#622826 - 04/30/02 01:51 AM (23 years, 1 month ago) |
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Very nice bro!
They look very nice!
Keep the pictures comin.
I am going to spawn the Un Id strain from Floriduh tomorrow in some horse poo.
Should be interesting to watch it on the poo.
Thanks again for the agar wedge!
--------------------
Laterz, Road
Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!
Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.
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pleezr
fuct
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Re: Un Id strain from the wilds of Florida. (4/14) [Re: Roadkill]
#622891 - 04/30/02 03:50 AM (23 years, 1 month ago) |
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YEP YEP!
Used the wedge in one quart jar, then spawnd 7 other quarts with it. 3 jars were spawned with poo just a few hours ago. I?ll be sure to post some pics of the progress for ya Joshua!
Those are very nice shrooms their. Cant wait
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pleezr
"Tell me about the fucking golf shoes!!"
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Joshua
Holoman
Registered: 10/27/98
Posts: 5,398
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Re: Un Id strain from the wilds of Florida. (4/14) [Re: Joshua]
#623044 - 04/30/02 09:43 AM (23 years, 1 month ago) |
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An aerial shot.
The slacker.
Joshua
--------------------
The Shroomery Bookstore
Great books for inquiring minds!
"Life After Death is Saprophytic!"
|
angryshroom
Stranger
Registered: 12/18/01
Posts: 7,264
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Re: Un Id strain from the wilds of Florida. (4/14) [Re: Joshua]
#623229 - 04/30/02 12:46 PM (23 years, 1 month ago) |
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Yeah thats a good point actually. The styrofoam could have really harboured some contams in the structure of the terr. actually. Willamette is pretty damp and probably a good area for contams to flourish.
Your fruits are looking cool. Cant really compare them to anything cause you?ve never seen ?em before, but thats a sic thought..
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Skankfeet
journeyman
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Re: Un Id strain from the wilds of Florida. (4/14) [Re: angryshroom]
#623600 - 04/30/02 09:40 PM (23 years, 1 month ago) |
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Lookin good Joshua...hey throw us a pic with some item for scale so we can get a size of those lovelies
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I'm never SURE of anything
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Joshua
Holoman
Registered: 10/27/98
Posts: 5,398
Loc: The Matrix
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Re: Un Id strain from the wilds of Florida. (4/30) [Re: Joshua]
#623663 - 04/30/02 11:21 PM (23 years, 1 month ago) |
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Here are the final pics. 71.4 grams.
I went a little crazy w/ the pics. I haven?t fruited anything since late February.
Enjoy!
I wanted to get these picked and printed fast and didn?t want to mess w/ them in fear of contaminating the caps. I guess I was lazy...
Joshua
--------------------
The Shroomery Bookstore
Great books for inquiring minds!
"Life After Death is Saprophytic!"
|
angryshroom
Stranger
Registered: 12/18/01
Posts: 7,264
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Re: Un Id strain from the wilds of Florida. (4/30) [Re: Joshua]
#624062 - 05/01/02 01:05 PM (23 years, 1 month ago) |
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Are you going to clone the big one? If not, why? Im trying to learn about cloning, and the right situations in doing so.
If you cloned the big one, theoretticly, couldn?t you get a dense flush next time? And then you wouldn?t have to deal with spores...?
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Joshua
Holoman
Registered: 10/27/98
Posts: 5,398
Loc: The Matrix
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Re: Un Id strain from the wilds of Florida. (4/30) [Re: angryshroom]
#624133 - 05/01/02 02:49 PM (23 years, 1 month ago) |
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I am majoring in Biochemistry/Biophysics, I hope I explain this correctly.
The fruits you see are from a fruit that grew from a multispore isolate on agar. Since it was from a fruit it is diploid, meaning it has two sets of genetic material. The fruit you see has the same set of genetic material. By cloning the fruit I would not accomplish anything since I already have an isolate of it growing on agar. What would be more beneficial would be to take the print from the clone which would be haploid (one set of genes) and grow it out either alone or with another print from the same strain. This would allow the different sets of haploid mycelia to mate creating a different dipliod fruit. I could then clone that fruit and work from there. By repeated selection I would eventually get a gene pool rich in desired traits.
Ultimately creating a good strain through cloning requires several sets of srong genetic material. You would want to take prints from the strongest fruits and mate them together. By cloning a fruit you are bottlenecking the gene pool. You are only allowing the more desireable genes to propogate. In my opinion one can not rely on clones to grow consistantly, maybe for a generation or two. Eventually the strain will start to senesce? sp. Senesce refers to the degradation of a strain due to being grown too long on the same substrate. I have no idea whether the last couple of sentences is true. I base my opinion on the fact that a healthy population usually has genetic diversity. What I intend to do is create a strain that has good diversity but also has been selected for desireable growing characteristics.
So all in all, cloning and genetics is too deep of a topic for me to cover. My advice to you is:
Select strong fruits and clone them if you want consistant and coordinated flushes. If you want continued strength and diversity in your fruits, mix genes by using multispore prints from different clones.
Again, this info is from my limited knowledge, please take it as so. It would be great if Anno could offer some of his advice and expertise on the subject also.
Joshua
--------------------
The Shroomery Bookstore
Great books for inquiring minds!
"Life After Death is Saprophytic!"
Edited by Joshua (05/01/02 02:56 PM)
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angryshroom
Stranger
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Re: Un Id strain from the wilds of Florida. (4/30) [Re: Joshua]
#624315 - 05/01/02 07:27 PM (23 years, 1 month ago) |
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Ok Joshua, thats making some sense. Thanks for the detailed reply!
I?ve purchased the TMC a while ago, read everything they said about cloning, and I have somewhat of a grasp on what to do. They dont go into the gene pool topic too much, if not any. But basically, I was thinking if I could clone the biggest fruit from my multispore flush, and create some spawn with the agar. Do this, get the fruits, and if I tried cloning the biggest fruit from that, and did it again, would that work. Hm I need to read more I guess. I can?t find too many good links on this however.
Im just trying to figure out the methods of preparing agar now.
Would you mind explaining your agar preperation?
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GGreatOne234
Stranger
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 Re: Un Id strain from the wilds of Florida. (4/14) [Re: Joshua]
#624405 - 05/01/02 09:27 PM (23 years, 1 month ago) |
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Amazing Psilocybe Cubensis Photos Joshua!!!!
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Joshua
Holoman
Registered: 10/27/98
Posts: 5,398
Loc: The Matrix
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Re: Un Id strain from the wilds of Florida. (4/30) [Re: angryshroom]
#624632 - 05/02/02 04:10 AM (23 years, 1 month ago) |
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If you clone a clone you get the same clone as you started with. This would not be advised as it would be a waste of time.
Buying a genetics text book might help you to understand. I find genetics to be very interesting as it is easy to see the effects of in our everyday lives.
Joshua
--------------------
The Shroomery Bookstore
Great books for inquiring minds!
"Life After Death is Saprophytic!"
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Joshua
Holoman
Registered: 10/27/98
Posts: 5,398
Loc: The Matrix
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 Re: Un Id strain from the wilds of Florida. (4/30) [Re: Joshua]
#633629 - 05/16/02 06:44 PM (23 years, 1 month ago) |
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"Life After Death is Saprophytic!"
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Joshua
Holoman
Registered: 10/27/98
Posts: 5,398
Loc: The Matrix
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Re: Un Id strain from the wilds of Florida. (4/30) [Re: Joshua]
#634392 - 05/17/02 11:46 AM (23 years, 1 month ago) |
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Weight of second flush was 89.9 grams wet.
I cased the tray again w/ contaminated casing. This is a pic. I got green mold as well as cob web mold. I pushed casing into the gaps in the sides of the tray. Needless to say I had contamination all over. I am currently treating the tray to rid the contamination. As soon as I am sure the contamination has been neutralised I will break the substrate up and recase. You all should see pictures w/i the next week of my healed casing.
The white spots you see would have matured to nastey green mold, the cob web mold is pretty aparent.
Joshua
--------------------
The Shroomery Bookstore
Great books for inquiring minds!
"Life After Death is Saprophytic!"
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