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Just a few quick questions from a new guy
    #7507573 -

First of all thanks to anyone who spends the time to read and respond to this thread!

I'm new at growing and have a few questions to make sure I'm doing everything correctly (i don't want to waste a bunch of my time). Below is a list of what has been done so far and what i am planning on doing, if anyone has any suggestions or comments please let me know.

Mix:
I baked brown rice flour (organic) and vermiculite in the oven for like 20 Min's at 400 degrees and boiled the water that i used in the mix. I then wiped all jars, lids, etc. with clorox disinfecting wipes (then dried them with paper towels and obviously air) after which i added the mix (ratio wise was 1 part water, 1 part flour, 2 2/3 part vermiculite). I placed a piece of tin foil in each one making a small "pool" out of the remaining 3/4Th's of an inch in each jar and filled it with dry vermiculite. I then put the lids on and places two layers of breathable medical tape over the inoculation holes. I steamed them in a bath of boiling water for 40 Min's. I then left them over night to cool down. This brings me to my first question, i followed a guide i thought sounded reasonable but i wanted to know if everyone thought this was enough to make sure the contents were sterile? will the possible clorox residue ruin the whole process? and even if it does cause a problem could the cake not touching the glass still colonize and fruit like normal? (i did try to get as much off after cleaning as possible, i only realized the possible mistake well after i was done)

Inoculation:
I then built a glove box out of a regular cardboard box, kitchen gloves, saran wrap, and duct tape. I wiped most of the inside of the box down with 91% rubbing alcohol as well as every jar and syringe before placing them inside the box (through a small door flap cut in the box). I proceeded to wipe the tops of the jar and the needle prior and post every injection (as well as making sure to cap the needle whenever i laid the syringe down) with the alcohol to insure sterility. Each jar received approx 1cc some a little more some a little less. I then wrapped each individual jar in tin foil to keep light out and to promote a sterile transfer (i made the jars in a different house then where they will be growing).

This is as far as i have gotten so far, as i said before i am curious to see if people think i did this correctly or totally messed up?

Birthing/Fruiting:
I have built myself a container to contain the jars during inoculation as well as the cakes during birthing and fruiting. My idea is basically a large plastic tub (with a relatively secure top, purchased at target) with a pool of water about 2 inch's deep with a submerged 300 watt fish tank heater and tube that are attached to a small air pump to disturb the water (for humidity and more uniform heating) and metal grates to hold the cakes almost an inch above the water line. The air pump runs from 9am to 4pm daily (i currently have it set up to test how well it works) and it seems to keep the humidity at 90%, and although the heater is relatively new i think the temp is staying right around 70 degrees.

My questions are basic, is it OK to allow the jars to colonize in such a humid environment? (i want to use the chamber cause of the temperature, room temperature is starting to get cold where i live and flux badly day and night). How sterile does the chamber have to be during birthing/fruiting? right now its filled with regular bath water and Ive had my hands in it setting it up to test. Is it OK that the heater is in the middle and although it is underwater and will hopefully distribute the heat pretty evenly it might make the center cakes marginally warmer then the rest? is the air pump worth having? it only raises the humidity a little bit but i thought it was a good addition to keep somewhat fresh air in the chamber. Is the birthing/fruiting process going to smell? I'm kind of hiding it at the moment so a strong oder (which i haven't seen mentioned anywhere but I'm still curious) would be an annoyance. My final question is if everything works out how much should i expect from 12 pint size mason jars with B+ strain in them? oh and what is the deal with dunking the cakes to get more flushes out of them, could someone please explain that to me or send me a link with a little more detailed description of the process please?

Again thanks to anyone who takes the time to read this long writing and respond to my questions. Any help is greatly appreciated!

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Re: Just a few quick questions from a new guy [Re: Grandmassab]
    #7507622 -

Why the hell would you put tin foil in the jars? You can throw dry verm right on top of your substrate without a problem.

If you've got a tub with water in it, buy another tub, and put it in the one with the water in it. Ziptie or find some other way to tie it down if you have to. this is your basic tub-in-tub (TiT as you'll see it called around here a lot). It's usually only used for incubating. As for incubating the jars in high humidity, you're not really hurting anything, but not really helping with anything either, since a jar does most of the work at keeping the adequate amounts of humidity in unbirthed cakes.

Having standing water in a fruiting chamber is a really bad idea. It invites all kinds of nasty contaminations. Your best bet is to use perlite for humidity and fish tank bubblers to help with FAE.

During fruiting contamination isn't much of an issue; the mycelial network already has a strong hold over most of the nutrients a substrate can offer.

The only time anything in the process should smell like anything is if you've had something contam, and even then, it's not likely to make a room smell unless you let it go crazy and grow legs and try to conquer Earth. It'll smell a little earthy, and like mushrooms, but that's it, and it's not an unpleasant smell.

Your yields will vary depending on how things go with spores germinating. From one multispore syringe you can get all kinds of different results in each of your cakes. It all depends on which two kinds of spores are compatible with each other to reproduce. The only way to get good stable yields is using an isolated strain from something you've grown that had favorable characteristics. As for what you can expect, I wouldn't know.


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This post approved by:


Premedman1 said:
:lol: I just shat my pants.

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Re: Just a few quick questions from a new guy [Re: Grandmassab]
    #7507624 -

Im still workin on my first grow but i will put in some input. I dont know if your rich or not or just trying this out but i would suggest just using perlite for humidification and manually fanning. It really does work pretty good, its cheap as hell, and its easy. I live in a very dry enviornment and the temp. has gone from 30'sF a few days ago to 70'sF today so the temp does fluctuate alot, and its easy to maintain the required humidity with perlite.

Dunking is what you do to rehidrate your cakes because the mushrooms loose alot of water through evaporation/transpiration w/e and so after the cakes are birthed you dunk it and after each flush you dunk it. A dunk is putting a cake in water and submerging the whole cake (it will float) then puttin it in the fridge, i didnt put mine in the fridge but i would go with what is recommendid.
Information on dunking http://www.shroomery.org/5185/What-is-the-dunk-tek

The cakes will have a odor but its easily containable and cant even be smelled outside of my terrarium, but i dont have 12 cakes in mine.

Your sterilization/inoculation process seems good to me except i would have boiled my jars for atleast 1 hour. And you had a lid on the pan right? to keep in the steam?.
B+ strain is supposed to be able to handle lower temperatures, my room is at a constant temp of 65-72 and they are pinning (thank god, budda, muhammad, aquaman, jesus)

The grow guide i used.. http://www.a1b2c3.com/drugs/pictures/mmgg01.htm , except for the humidity, i used perlite. And dunked my cakes and rolled in verm


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"Its only after we've lost everything, that we are free to do anything"  <-- Brad Pitt? Fight Club

"Nothing is real, not you or me, we just think we are real, its a fantastic illusion, we exist only because something wants us too, why, I have no idea."  <-- 1138, DF2 debugger and teacher.

Edited by The_Bomb (10/11/07 02:10 AM)

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Re: Just a few quick questions from a new guy [Re: The_Bomb]
    #7507882 -

It all looks good. Except the standing water. 3-4 inchs of damp perlite should give u plenty humidity.

Usually, when steam sterilising u want to sterilise for 90 minutes. Though baking ur BRF and Verm will have helped in the process. I usually just make the mix up, load it into the jars, leaving room for 1/2 inch layer of verm, wipe the edge of the glass clean then load with dry ver, and on with the lid, then tinfoil over the jar, into the steamer.

The tinfoil boil, isnt really necessary but i suppose it will give an extra contam barrier, this may restrict gas exchange though.

Theres no need for humidity during incubation, all the moisture is within ur jar. Just keep it at room temperature, or no higher than 80F.

Theres no smell, not like growing weed, u could have dozens of jars incubating and not smell anything. Even once they are birthed u cant smell it unless u put a cake infront of ur face.

U will want to dunk your cakes in cold clean water for 24 hours then roll them in dry verm before sticking in the FC, this rehydrates the cake giving it more moisture to fruit from. U can do this after each flush.

yeilds vary, just to give u an indication, my first pf cake to fruit from multispore (strain was EQ) i got 18 grams wet, roughly 1.8 grams dry. I had a pretty strong trip of that. Generaly future flushes will give less shrooms but bigger ones.

Currently waiting on the second flush so cant tell u how many flush's you wil get, i think 3-4 if u get it absolutely right.


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek

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Re: Just a few quick questions from a new guy [Re: veda_sticks]
    #7507972 -

Ok so it seems to be in general consensus that i need to lose the set up i have now and replace it with the simple perlite. My question is then how do i keep the tempature stable? ive seen alot about heating pads but im skeptical about putting one under a plastic container (fire hazard, etc.) What is FAE? which was mentioned earlier.

Sigh i guess im going back to petsmart one more time lol, oh and just so everyone knows this set up is solely being used for Golden Oyster mushrooms :wink:

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Re: Just a few quick questions from a new guy [Re: Grandmassab]
    #7507998 -

Quote:
thank god, budda, muhammad, aquaman, jesus




how the hell did Muhammad get in that list?


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Lazy Drywall Tek (no powdery mess)

This series will blow your mind and confirm what you already know to be true.
The Pharmacratic Inquisition
Best Thread Ever ! ! !

:pm: me if you have questions about lasers

Although i may advise others in a general way regarding all types of mushroom grows, and may even post question from other forums about growing "active" mushrooms, i only grow non-"active" mushrooms and edibles.

FeelFamily resident tech guru

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Re: Just a few quick questions from a new guy [Re: Slimz]
    #7508061 -

sounds like you have done some reading...it bothers me when ppl try and sterilize jars without a PC...steam baths work but your chances of getting them completely sterilized is less, that is the one place i would not take a cheap way out of. get 20$ and buy a 6qt PC from walmart or whatever is close..thrift shops usualy have one or 2 for realy cheap. doing BRF jars are extremely simple, sometimes ppl read them too much and make it complicated which it is far from. just read the PF Tek and follow it, and you should be just fine..

good luck.


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"Most people don't know how they're gonna feel from one moment to the next. But a dope fiend has a pretty good idea. All you gotta do is look at the labels on the bottles." ~drugstore cowboy~

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Re: Just a few quick questions from a new guy [Re: Slimz]
    #7509135 -

Slimz said:
Quote:
thank god, budda, muhammad, aquaman, jesus




how the hell did Muhammad get in that list?


lol, i was just thanking every possible profit or w/e i could think of at the time, isnt the profit of the mormans john smith ? or something.


--------------------
"Its only after we've lost everything, that we are free to do anything"  <-- Brad Pitt? Fight Club

"Nothing is real, not you or me, we just think we are real, its a fantastic illusion, we exist only because something wants us too, why, I have no idea."  <-- 1138, DF2 debugger and teacher.

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Re: Just a few quick questions from a new guy [Re: The_Bomb]
    #7509157 -

Quote:
My question is then how do i keep the tempature stable? ive seen alot about heating pads but im skeptical about putting one under a plastic container (fire hazard, etc.) What is FAE? which was mentioned earlier.



NEVER heat a fruiting chamber. Normal room temperature is best for both colonization and fruiting. FAE is Fresh Air Exchange and you need LOT's of it, especially with oysters.
RR


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Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

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