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Amazon Shop for: ½ Pint Jars, Agar, Laminar Flow Hood, Malt Extract, Wild Bird Seed

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Invisibleangryshroom
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A few quick Panaeolus Tropicalis Q's
    #726628 - 07/06/02 03:55 PM (14 years, 5 months ago)

So I aquired a Panaelous Tropicalis print, and I am extremely excited about starting some.

A few quick questions...

1) How long do the spores take to germinate on grain. (going to do a multispore via syringe directly onto birdseed).

2) What is the optimum temperature for incubation?

3) Is it ok to shake the jars after the mycelium has established itself in some areas?

Now tell me if Im on the right track with this: I can spawn my birdseed into say a 50/50 mix of pasturized manure and straw. Should I spawn run this until completely colonized, THEN add a casing, or add a casing at the beginning.

Ive heard too that you dont even incubate the bulk substrate...is this true or a myth?

Thanks a lot guys.


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Anonymous

Re: A few quick Panaeolus Tropicalis Q's [Re: angryshroom]
    #728858 - 07/07/02 11:26 AM (14 years, 5 months ago)

Isolations from multispore germinations on agar do better in my opinion.

Shaking spawn during colonization of multispore innoculations has variable succes.

Temps for incubation are in the low 80's F.

Always let your substrate colonize completely before casing. It is better IMO.

colonize substrate, case very thin, let it recover for 24-36 hours, and initiate in fruiting chamber. Pans are fast USUALLY. From multispore, they can be delayed alot.


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Anonymous

Re: A few quick Panaeolus Tropicalis Q's [Re: ]
    #728862 - 07/07/02 11:27 AM (14 years, 5 months ago)

PS there are NO Pan. tropicalis in circulation as of yet in the OMC. They are just a unique Pan. cyan strain from south east Florida.


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Invisibleangryshroom
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Re: A few quick Panaeolus Tropicalis Q's [Re: ]
    #729183 - 07/07/02 01:57 PM (14 years, 5 months ago)

Wow, no real Pan. Trop print? How could you be so sure...? I guess I wouldn't be too suprised if that was true, but how could vendors be selling them labled as a Panaeolus Tropicalis print?

So basically, the cultivation parameters for this species is somewhat similar to cubensis in ways. However, mycelium is less strong and casing should be added very lightly. Ive heard air exchanges are crucial for sucess as well.

Well If I had enough money to buy a flowhood or found out how to construct one or have a glovebox, I would be doing agar, no dout about it. Ive tried to ask fellow shroomerites on the construction for a flowhood but with minimal feedback, which has really bummed me out.

I guess I could construct a really low-budge glovebox, but I donno if it would be worth going through the hassels of getting mostly contaminated plates.

Thanks for the reply's Teonan.


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InvisibleChampion des Champignons
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Re: A few quick Panaeolus Tropicalis Q's [Re: angryshroom]
    #730604 - 07/08/02 06:49 AM (14 years, 5 months ago)

IME, unless your substrate is wetter than normal, shaking results in the mycelium stalling.


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hmmm........


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Invisibleangryshroom
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Re: A few quick Panaeolus Tropicalis Q's [Re: Champion des Champignons]
    #730899 - 07/08/02 09:42 AM (14 years, 5 months ago)

Yeah I dont think Im going to shake the substrate with this species. I like the idea that I read in the grow/find section, that says to use 1/2 pint jars of birdseed with a vermeculite ramsey seal. Kinda like PF in a way I suppose.

Im going to try that out with a few and the normal polyfill way in quart jars.


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OfflineHammerloaf
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Re: A few quick Panaeolus Tropicalis Q's [Re: angryshroom]
    #731132 - 07/08/02 11:20 AM (14 years, 5 months ago)

angryshroom... why not use the oven tek.. works great, no need for a flow hood or glovebox.


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Invisibleangryshroom
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Re: A few quick Panaeolus Tropicalis Q's [Re: Hammerloaf]
    #731336 - 07/08/02 12:52 PM (14 years, 5 months ago)

I do, but I dont think it would be sufficent for agar....
?


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InvisibleCLuB99
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Re: A few quick Panaeolus Tropicalis Q's [Re: ]
    #731542 - 07/08/02 02:31 PM (14 years, 5 months ago)

In reply to:

PS there are NO Pan. tropicalis in circulation as of yet in the OMC. They are just a unique Pan. cyan strain from south east Florida.





ehm...actually i'm growing some real pantrops
i got the print from mushroom john, it says on the envelope "panaeolus tropicalis, malaysia, 1996"


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Anonymous

Re: A few quick Panaeolus Tropicalis Q's [Re: CLuB99]
    #733221 - 07/09/02 09:48 AM (14 years, 5 months ago)

Well they aren't in circulation yet!!! I have learned quickly to call into question any and all information relating to taxonomy of the potent Pan. complex. Lots of conflicting information and POOR identification skills run rampant in the OMC.

I hope they are Pan. trops, but I am not gonna hold my breath. MJ actually refered MR.G to HAWK's Pan. trop for identification comparison. Not realizing that HAWK got his Pan. trop from Mr. G. This is the Pan. trop that is in circulation in the OMC right now, and it is 4-spored as opposed to 2- spored, which makes it impossible for it to be a Pan. tropicalis.

I hope it is a Pan. tropicalis you got there from MJ. I'm just a tad bit skeptical.



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Invisiblemickey_rourke
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Re: A few quick Panaeolus Tropicalis Q's [Re: angryshroom]
    #767881 - 07/22/02 03:58 PM (14 years, 4 months ago)

You don't need a flowhood. The oven tek works fine if you don't mind a loss every now and then (i.e. over 90% success rate.)


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"I tried to put it all behind me, but my redneck past is nipping at my heels.." -- Ben Folds Five


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InvisibleChampion des Champignons
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Re: A few quick Panaeolus Tropicalis Q's [Re: mickey_rourke]
    #768797 - 07/22/02 09:01 PM (14 years, 4 months ago)

oven tek shmoven tek
if you're using peroxidated agar, and are smooth and quick with your transfers no sort of flowhood/"tek" is necessary, though it can't hurt..


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hmmm........


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Invisibleangryshroom
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Re: A few quick Panaeolus Tropicalis Q's [Re: Champion des Champignons]
    #770960 - 07/23/02 05:07 PM (14 years, 4 months ago)

Peroxidized agar? Shit, whats the difference? Where do you guys buy your peroxidized agar anyways?


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OfflineAnnoA
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Re: A few quick Panaeolus Tropicalis Q's [Re: angryshroom]
    #770995 - 07/23/02 05:20 PM (14 years, 4 months ago)



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OfflineDante606
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Re: A few quick Panaeolus Tropicalis Q's [Re: angryshroom]
    #771204 - 07/23/02 06:56 PM (14 years, 4 months ago)

In reply to:

So basically, the cultivation parameters for this species is somewhat similar to cubensis in ways. However, mycelium is less strong and casing should be added very lightly. Ive heard air exchanges are crucial for sucess as well.





Very few ways from what I have read...


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Offlinestaindblue
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Re: A few quick Panaeolus Tropicalis Q's [Re: angryshroom]
    #771331 - 07/23/02 07:38 PM (14 years, 4 months ago)

Check out mycotopia's do it yourself projects section...they have a design for building your own flow hood. This is something i want to build too http://www.mycotopia.net/


Edited by staindblue (07/23/02 07:49 PM)


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Invisibleangryshroom
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Re: A few quick Panaeolus Tropicalis Q's [Re: Anno]
    #771689 - 07/23/02 09:27 PM (14 years, 4 months ago)

Great Anno, thanks for the link.

So if I was to buy Agar from spore works, which one would I need to buy?

The Malt Extract Agar - light or wheat? And would I have to also buy just the agar agar, or would just hte Malt Extract agar be what im looking for?


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Anonymous

Re: A few quick Panaeolus Tropicalis Q's [Re: angryshroom]
    #772290 - 07/24/02 06:28 AM (14 years, 4 months ago)

- Post History Deleted Upon User's Request -


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Amazon Shop for: ½ Pint Jars, Agar, Laminar Flow Hood, Malt Extract, Wild Bird Seed

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

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