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Culturing edibles from tissue
    #698325 -

I'd like to try and culture some edibles from tissue samples on agar. Please forgive my ignorance on the subject, but I have some questions.

(1) Can I use tissue from fresh, market mushrooms?
(2) I'm assuming I just put a wafer thin sliver of mushroom onto agar?

I'll go looking for some books on the subject. Any pointers to FAQs or Internet sites muchly appreciated.

-psyconaut


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It may look like a button mushroom right now, but wait until you see how it grows!

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Re: Culturing edibles from tissue [Re: psyconaut]
    #698438 -

dad go buy the mushroom cultivator -what kind of cultures are you looking for?

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Re: Culturing edibles from tissue [Re: psyconaut]
    #698581 -

Do buy TMC and GGMM.

But-yep,you can use a piece of a fresh mushroom.Unless you are fairly good at sterile technique,it is tricky to get a clean culture that way.Dipping some tissue in peroxide,then shredding a piece from inside that can help,as will peroxidated agar.

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Re: Culturing edibles from tissue [Re: shaggymane]
    #698633 -

Portabellos....maybe others. I have a hankering for the big wide caps.

Thanks for the advice guys.

-psyconaut


--------------------
It may look like a button mushroom right now, but wait until you see how it grows!

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Re: Culturing edibles from tissue [Re: psyconaut]
    #698946 -

I've heard a few people saying that they tried to clone different mushrooms bought from the store with no success. Someone was saying that big producers use strains that dont reproduce easily


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There is always a need for intoxication: China has
opium, Islam has hashish, the West has woman."
Andr? Malraux

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Re: Culturing edibles from tissue [Re: psyconaut]
    #699184 -

>(1) Can I use tissue from fresh, market mushrooms?
Yes.

(2) I'm assuming I just put a wafer thin sliver of mushroom onto agar?
No.
Read http://www.mykoweb.com/articles/cultivation.html .
Also http://shroomery.org/faq/faq.php?list=category&nr=11&prog=1&lang=en&onlynewfaq=1 .

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Re: Culturing edibles from tissue [Re: stan]
    #711484 -

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