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The Tissue Culture (Cloning):

Once you have living cultures you can keep living cultures on hand by doing live tissue cultures to agar plates. All you need is a living culture of fungus and MEA petri dishes. For me this works well because I can cut the cultured plates into 8 sections, sterilize 7 jars of grain and sue the last wedge of agar to culture about 3-5 plates of fresh MEA. I always have some cultures as a backup and I can cultivate as much as I want of a specific culture.

Here is how it is done:

- Carefully unwrap the Parafilm or plastic wrap off the petri dishes and stack off to the side.

- Choose a culture that you wish to use for the transfer.

- Sterilize a scalpel (a exacto knife will work for this) and cool the knife on the plate that you wish to inoculate.

- Carefully cut a sterile piece of the culture that you want to clone and remove the cultured piece with the sterile knife.

- Place the tissue on the agar plate with the knife, and replace the lid. Seal with Parafilm of plastic wrap to prevent contamination.

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