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Invisiblepsyconaut
NASA trainedpsychonaut
Registered: 05/22/02
Posts: 617
Loc: The Great White North
Culturing edibles from tissue
    #698325 - 06/24/02 12:23 PM (21 years, 8 months ago)

I'd like to try and culture some edibles from tissue samples on agar. Please forgive my ignorance on the subject, but I have some questions.

(1) Can I use tissue from fresh, market mushrooms?
(2) I'm assuming I just put a wafer thin sliver of mushroom onto agar?

I'll go looking for some books on the subject. Any pointers to FAQs or Internet sites muchly appreciated.

-psyconaut


--------------------
It may look like a button mushroom right now, but wait until you see how it grows!


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Invisibleshaggymane
PHARMER

Registered: 03/11/02
Posts: 514
Loc: great white north
Re: Culturing edibles from tissue [Re: psyconaut]
    #698438 - 06/24/02 01:36 PM (21 years, 8 months ago)

dad go buy the mushroom cultivator -what kind of cultures are you looking for?


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Offlineaural
Hola Amigos!
Registered: 05/03/00
Posts: 511
Loc: The Desert In The Spring
Last seen: 19 years, 10 months
Re: Culturing edibles from tissue [Re: psyconaut]
    #698581 - 06/24/02 03:22 PM (21 years, 8 months ago)

Do buy TMC and GGMM.

But-yep,you can use a piece of a fresh mushroom.Unless you are fairly good at sterile technique,it is tricky to get a clean culture that way.Dipping some tissue in peroxide,then shredding a piece from inside that can help,as will peroxidated agar.


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Invisiblepsyconaut
NASA trainedpsychonaut
Registered: 05/22/02
Posts: 617
Loc: The Great White North
Re: Culturing edibles from tissue [Re: shaggymane]
    #698633 - 06/24/02 03:42 PM (21 years, 8 months ago)

Portabellos....maybe others. I have a hankering for the big wide caps.

Thanks for the advice guys.

-psyconaut


--------------------
It may look like a button mushroom right now, but wait until you see how it grows!


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Offlinestan
member
Registered: 09/15/00
Posts: 99
Loc: Sydney Australia
Last seen: 21 years, 6 months
Re: Culturing edibles from tissue [Re: psyconaut]
    #698946 - 06/24/02 06:43 PM (21 years, 8 months ago)

I've heard a few people saying that they tried to clone different mushrooms bought from the store with no success. Someone was saying that big producers use strains that dont reproduce easily


--------------------
There is always a need for intoxication: China has
opium, Islam has hashish, the West has woman."
Andr? Malraux


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OfflineAnnoA
Experimenter
 User Gallery

Folding@home Statistics
Registered: 06/17/99
Posts: 24,166
Loc: my room
Last seen: 25 minutes, 44 seconds
Re: Culturing edibles from tissue [Re: psyconaut]
    #699184 - 06/24/02 08:20 PM (21 years, 8 months ago)

>(1) Can I use tissue from fresh, market mushrooms?
Yes.

(2) I'm assuming I just put a wafer thin sliver of mushroom onto agar?
No.
Read http://www.mykoweb.com/articles/cultivation.html .
Also http://shroomery.org/faq/faq.php?list=category&nr=11&prog=1&lang=en&onlynewfaq=1 .


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Anonymous

Re: Culturing edibles from tissue [Re: stan]
    #711484 - 06/29/02 04:22 PM (21 years, 7 months ago)

- Post History Deleted Upon User's Request -


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