Home | Community | Message Board


Azarius
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Gourmet and Medicinal Mushrooms

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop:   Original Seeds Store Buy CBD, Cannabis Seeds, Compare CBD   Amazon Agar, Paul Stamets

Jump to first unread post. Pages: 1
Invisiblepsyconaut
NASA trainedpsychonaut
Registered: 05/22/02
Posts: 617
Loc: The Great White North
Culturing edibles from tissue
    #698325 - 06/24/02 02:23 PM (14 years, 10 months ago)

I'd like to try and culture some edibles from tissue samples on agar. Please forgive my ignorance on the subject, but I have some questions.

(1) Can I use tissue from fresh, market mushrooms?
(2) I'm assuming I just put a wafer thin sliver of mushroom onto agar?

I'll go looking for some books on the subject. Any pointers to FAQs or Internet sites muchly appreciated.

-psyconaut


--------------------
It may look like a button mushroom right now, but wait until you see how it grows!


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibleshaggymane
PHARMER

Registered: 03/11/02
Posts: 514
Loc: great white north
Re: Culturing edibles from tissue [Re: psyconaut]
    #698438 - 06/24/02 03:36 PM (14 years, 10 months ago)

dad go buy the mushroom cultivator -what kind of cultures are you looking for?


Post Extras: Print Post  Remind Me! Notify Moderator
Offlineaural
Hola Amigos!
Registered: 05/04/00
Posts: 511
Loc: The Desert In The Spring
Last seen: 13 years, 1 month
Re: Culturing edibles from tissue [Re: psyconaut]
    #698581 - 06/24/02 05:22 PM (14 years, 10 months ago)

Do buy TMC and GGMM.

But-yep,you can use a piece of a fresh mushroom.Unless you are fairly good at sterile technique,it is tricky to get a clean culture that way.Dipping some tissue in peroxide,then shredding a piece from inside that can help,as will peroxidated agar.


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblepsyconaut
NASA trainedpsychonaut
Registered: 05/22/02
Posts: 617
Loc: The Great White North
Re: Culturing edibles from tissue [Re: shaggymane]
    #698633 - 06/24/02 05:42 PM (14 years, 10 months ago)

Portabellos....maybe others. I have a hankering for the big wide caps.

Thanks for the advice guys.

-psyconaut


--------------------
It may look like a button mushroom right now, but wait until you see how it grows!


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinestan
member
Registered: 09/16/00
Posts: 99
Loc: Sydney Australia
Last seen: 14 years, 9 months
Re: Culturing edibles from tissue [Re: psyconaut]
    #698946 - 06/24/02 08:43 PM (14 years, 10 months ago)

I've heard a few people saying that they tried to clone different mushrooms bought from the store with no success. Someone was saying that big producers use strains that dont reproduce easily


--------------------
There is always a need for intoxication: China has
opium, Islam has hashish, the West has woman."
Andr? Malraux


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineAnnoA
Experimenter
 User Gallery

Folding@home Statistics
Registered: 06/18/99
Posts: 24,122
Loc: my room
Last seen: 1 month, 3 days
Re: Culturing edibles from tissue [Re: psyconaut]
    #699184 - 06/24/02 10:20 PM (14 years, 10 months ago)

>(1) Can I use tissue from fresh, market mushrooms?
Yes.

(2) I'm assuming I just put a wafer thin sliver of mushroom onto agar?
No.
Read http://www.mykoweb.com/articles/cultivation.html .
Also http://shroomery.org/faq/faq.php?list=category&nr=11&prog=1&lang=en&onlynewfaq=1 .


Post Extras: Print Post  Remind Me! Notify Moderator
Anonymous

Re: Culturing edibles from tissue [Re: stan]
    #711484 - 06/29/02 06:22 PM (14 years, 10 months ago)

- Post History Deleted Upon User's Request -


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Shop:   Original Seeds Store Buy CBD, Cannabis Seeds, Compare CBD   Amazon Agar, Paul Stamets

Mushrooms, Mycology and Psychedelics >> Gourmet and Medicinal Mushrooms

Similar ThreadsPosterViewsRepliesLast post
* Guys need your help here. Trying to pick edible strain to grow. DayzeoftheNu 1,446 13 03/09/09 01:27 AM
by DayzeoftheNu
* R. Wayne's Peroxide Technique: Mold! Basidiocarp 2,269 11 02/24/05 02:29 PM
by debianlinux
* The best source for spores and cultures Fucknuckle 2,175 9 08/17/04 10:36 AM
by eric_the_red
* What's your favorite edible strain? MycoThrill 859 2 09/17/07 09:48 AM
by greys
* anyone wanna swap some edibles? mattymonkey 1,056 7 07/28/05 12:36 AM
by mushrx1
* oyster liquid culture/straw tea question PinheadX 1,325 1 06/25/08 06:02 PM
by waixingren
* edible syringe or print mikey 854 3 08/27/03 11:46 AM
by koraks
* My first edibles :)
( 1 2 all )
G a n j a 3,183 21 02/05/03 01:42 AM
by zeronio

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: RogerRabbit, cronicr, Pastywhyte
1,455 topic views. 2 members, 7 guests and 4 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Marijuana Demystified
Please support our sponsors.

Copyright 1997-2017 Mind Media. Some rights reserved.

Generated in 0.021 seconds spending 0.004 seconds on 16 queries.