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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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A bit unclear on gas exchange and incubation
    #7041339 -

My BRF jars were inoculated yesterday morning, and I've still got two layers of heavy duty foil on them (I took them off to inoculate and then replaced them).  Each jar has 4 3/32" holes.

I've been reading all kinds of old threads on gas exchange and there seem to be differing opinions (like anything else lol).  Some say there is no reason for the foil after sterilization, some disagree. Some say loosen the jar lids, some say no.  I've read that no gas exchange is necessary before there is mycellium, but that will change soon (hopefully).

My questions:

Is there a disadvantage to removing one layer of foil and loosening the other, to keep an additional contaminant barrier?  I do have a 1/2" dry verm barrier.  Or should I just get rid of the foil altogether?

I made a TiT incubator but the temps are reading 80-81 so I've not yet filled it with water or turned the heater on, so right now it's just jars in a rubbermaid inside another (larger) rubbermaid (both with lids on.)  I open it (or plan to) 1-2 times a day to check temps or progress.  Is there anything else I need to do to ensure adequate gas exchange?

And instead of starting another thread I'll ask this here:

I ended up with some extra substrate, so I filled an 11th jar with what I had left.  It ended up being about 5/8s to 2/3s full, and I filled it to the top with dry verm (about an inch and a half of verm).  Is this going to cause a problem or should I just see what happens?

Thank you for any knowledge you can provide me with. I'm really impressed by this community :thumbup:


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People seem not to see that their opinion of the world is also a confession of their character.

    ~Ralph Waldo Emerson

:amanitajar: :rose:

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Re: A bit unclear on gas exchange and incubation [Re: veilbreaker]
    #7041372 -

Taking off one layer and loosening the other layer will work fine for you. Now in the 11th jar you shot up, There's allot of Verm in there correct? when you inoculated that one i think the spore solution went straight into the verm and not the Substrate. I would wait it out.

And everything else with your incubator is fine.

Edited by DiabloSmurf (06/13/07 10:22 AM)

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Re: A bit unclear on gas exchange and incubation [Re: veilbreaker]
    #7041380 -

Is there a disadvantage to removing one layer of foil and loosening the other, to keep an additional contaminant barrier? I do have a 1/2" dry verm barrier. Or should I just get rid of the foil altogether?

If you have the dry verm layer, ditch the foil altogether, it's not necessary. If you are still bothered by it, then put band-aids over the holes.

I made a TiT incubator but the temps are reading 80-81 so I've not yet filled it with water or turned the heater on, so right now it's just jars in a rubbermaid inside another (larger) rubbermaid (both with lids on.) I open it (or plan to) 1-2 times a day to check temps or progress. Is there anything else I need to do to ensure adequate gas exchange?

No need to check temps or progress that many times a day. Stick the jars in there, and then don't check it for a week at a time. Trust me, waiting longer to check the progress will keep you from going insane.

I ended up with some extra substrate, so I filled an 11th jar with what I had left. It ended up being about 5/8s to 2/3s full, and I filled it to the top with dry verm (about an inch and a half of verm). Is this going to cause a problem or should I just see what happens?

It's fine. The reason people go within an inch of the top of the jar is to ensure that the innoc needle goes all the way through the dry layer to the substrate. As long as your needle will make it the whole way, you should be good.

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Re: A bit unclear on gas exchange and incubation [Re: DiabloSmurf]
    #7041477 -

DiabloSmurf said:
There's allot of Verm in there correct? when you inoculated that one i think the spore solution went straight into the verm and not the Substrate.



Yeah that's what I was thinking.  After I inoculated (and started reading a bit more about inoculation) I realized that a lot of the time I didn't push the needle all the way in.  I just pushed it until I could feel it touch the glass and injected.  So sometimes the needlepoint was probably still in the verm.  :frown: 

I was focused on being quick with my inoculations so maybe I didn't take enough care.  We'll see how they colonize.

Thanks.


--------------------
People seem not to see that their opinion of the world is also a confession of their character.

    ~Ralph Waldo Emerson

:amanitajar: :rose:

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Re: A bit unclear on gas exchange and incubation [Re: veilbreaker]
    #7041485 -

No problem. It will be alright. We'll just see how it goes. don't worry to much. And goodluck :smile:

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Re: A bit unclear on gas exchange and incubation [Re: GoodbyeOrb]
    #7041493 -

GoodbyeOrb said:
I open it (or plan to) 1-2 times a day to check temps or progress.  Is there anything else I need to do to ensure adequate gas exchange?

No need to check temps or progress that many times a day.  Stick the jars in there, and then don't check it for a week at a time.  Trust me, waiting longer to check the progress will keep you from going insane.



Yeah I gather that noob impatience is pretty common... I just want to make sure that my setup is giving the right temps and everything. Also, I want to catch any contams quickly, but yeah I'll take a chill pill and forget about it for a few days.

:rose:


--------------------
People seem not to see that their opinion of the world is also a confession of their character.

    ~Ralph Waldo Emerson

:amanitajar: :rose:

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Re: A bit unclear on gas exchange and incubation [Re: veilbreaker]
    #7041766 -

Work on implementing correct filtering techniques on your jar lids in the future, it will pay off in spades - tyvek, micropore tape, polyfill, whatever your choice.

Also just an addendum incubating over 75 degrees increases the likelihood of contamination taking over the colonization battle. You must also factor in a 5 degree temperature increase thrown off by the progression of the organism itself. Anything over 85 degrees and the mycelium will be weakened and damaged after repeated exposure, so watch the temps as you are probably getting up there with the Incubator set to 80.

I was in your situation early on and found that taking off the foil, taping the holes and dropping my FC temps greatly reduced failure rates. YMMV.

Edited by xeallos (06/13/07 12:41 PM)

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Re: A bit unclear on gas exchange and incubation [Re: xeallos]
    #7042010 -

like others have said, remove all the foil. I don't remember who did the experiment, but someone on here took 100 pf jars and left the foil on half of them during incubation. All 50 of the jars w/o the foil colonized faster..

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Re: A bit unclear on gas exchange and incubation [Re: xeallos]
    #7042921 -

xeallos said:
I was in your situation early on and found that taking off the foil, taping the holes and dropping my FC temps greatly reduced failure rates. YMMV.



I assume you're talking about micropore tape... I only have masking tape on hand (and my supplies budget is expended for now). Is that any kind of substitute? Nobody else said anything about taping the holes after removing the foil... is it necessary to prevent contams?


--------------------
People seem not to see that their opinion of the world is also a confession of their character.

    ~Ralph Waldo Emerson

:amanitajar: :rose:

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Re: A bit unclear on gas exchange and incubation [Re: veilbreaker]
    #7043024 -

lol why would you tape the holes,there is supposed to be a dry layer of verm on top to act as a barrier for contams putting tape over the holes would only hinder gas exchange no need for that,just remove the alum foil and let em do there thing,as for dropping fc temps 70-75 is fine it is true that contams thrive at high temps but it is also true that they thrive in stale air best way to keep a contam out of the fc is by having alot of fae.


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"Anything i say is fictional"
  what you should look for in manure

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Re: A bit unclear on gas exchange and incubation [Re: xaxphaanes]
    #7043133 -

Remove the foil. If you have micropore tape, you can put it over the holes. If not, leave them open. Don't use masking tape. You want gas exchange during colonization, but it doesn't have to be much. The four inoculation holes are your gas exchange holes and the dry vermiculite is your filter. Keep your jars at room temperature until one week past full colonization, and then birth, dunk and roll. You have three weeks to go until then.
RR


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Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
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Re: A bit unclear on gas exchange and incubation [Re: RogerRabbit]
    #7043143 -

Done. Thanks guys!

*stares at clock*


j/k


--------------------
People seem not to see that their opinion of the world is also a confession of their character.

    ~Ralph Waldo Emerson

:amanitajar: :rose:

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