The Lizard,

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My mind is corrosive...

Another quick question. When using a pressure cooker, if you stack the jars, do you still just fill up the water to about 1/3 of the way up the Bottem jar?

thanks guys!

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"A life without cause is a life without effect" - Dildano, 'Barbarella'

• Punched holes in jar lids
  
• Filled Jars w/ substrate
  
• Placed lids on jar, rubber side towards glass
  
• Covered holes with paper masking tape.(tip, fold 1/2" of tape back on itself to make pull tab)
  
• Covered top of jar/lid combo with foil (to keep water from accumulating on lid)
  
• Placed in pressure cooker and cooked for 1 hour
  
• Allowed to cool to room temp overnight
  
• Went to dining room, turned off A/C & fan
  
• Wiped table/chairs down with rubbnig alcohol
  
• placed shot glass upside down on table
  
• filled botton of shot glass with rubbing alchol
  
• Placed jars on table with foil still in place
  
• Grabed syringe from fridge and shaked up spores
  
• Covered face with hanky
  
• removed foil from one jar
  
• removed cover on needle
  
• lit alchol in shot glass
  
• sterilized needle in flame till glowed
  
• pushed plunger a little to cool needle
  
• peeled back tape on one hole
  
• injected 1/4 cc
  
• replaced tape
  
• repeated for remaining holes and jars
  
• Placed innoculated jars back in box the came in
  

Disclamer: I've only done 6 jars my entire life, all 6 jars grew w/o any contamination.

Theory:
When jars are placed in cooker and cooker sealed. The jars and inside of cooker are at room pressure. As you heat cooker, pressure inside cooker rises, since jars are kind of sealed, pressure in jars remains at room pressure. After cooker is turned off, pressure inside cooker falls back to room pressure. If jars were possibly pressurized, due to leak on tape seal over holes, pressure would leak back out same way it got in (thru taped holes)

Side note:
Substrate is to be moist, moist Should be easly confused with dry. After innocculating, I was concerned that perhaps my substarte was too dry, but I left it alone. Once colinization took place, I could see water droplettes start to form on inside of glass.

Peace
/Gadget